##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434106
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13700340469839	33.0	31.0	34.0	30.0	34.0
2	32.25452999958536	33.0	31.0	34.0	30.0	34.0
3	32.30508447245604	34.0	31.0	34.0	30.0	34.0
4	35.84785973932634	37.0	35.0	37.0	35.0	37.0
5	35.89298466273215	37.0	35.0	37.0	35.0	37.0
6	35.916034332628435	37.0	35.0	37.0	35.0	37.0
7	36.08381132718737	37.0	35.0	37.0	35.0	37.0
8	35.7891643976356	37.0	35.0	37.0	35.0	37.0
9	37.52575407849696	39.0	37.0	39.0	35.0	39.0
10	37.28125619088426	39.0	37.0	39.0	34.0	39.0
11	37.21271302400796	39.0	37.0	39.0	34.0	39.0
12	37.086951113322556	39.0	37.0	39.0	34.0	39.0
13	37.08021082408444	39.0	37.0	39.0	33.0	39.0
14	38.2279074696042	40.0	37.0	41.0	34.0	41.0
15	38.373551621032654	40.0	37.0	41.0	34.0	41.0
16	38.46865051392978	40.0	38.0	41.0	34.0	41.0
17	38.40491953578158	40.0	37.0	41.0	34.0	41.0
18	38.38702989592404	40.0	37.0	41.0	34.0	41.0
19	38.40354890280254	40.0	37.0	41.0	34.0	41.0
20	38.28290555762878	40.0	37.0	41.0	34.0	41.0
21	38.280281774497475	40.0	37.0	41.0	34.0	41.0
22	38.25886073908216	40.0	37.0	41.0	34.0	41.0
23	38.294580125591445	40.0	37.0	41.0	34.0	41.0
24	38.19873947837625	40.0	37.0	41.0	34.0	41.0
25	38.122239729467	40.0	36.0	41.0	34.0	41.0
26	37.97909036041889	40.0	36.0	41.0	34.0	41.0
27	37.937694940866976	40.0	36.0	41.0	34.0	41.0
28	37.957752254057766	40.0	36.0	41.0	34.0	41.0
29	37.95643690711485	40.0	36.0	41.0	34.0	41.0
30	37.815268621028046	40.0	36.0	41.0	33.0	41.0
31	37.84123923649984	40.0	36.0	41.0	34.0	41.0
32	37.77021510875224	40.0	36.0	41.0	33.0	41.0
33	37.75078206705275	40.0	36.0	41.0	33.0	41.0
34	37.66000930648275	40.0	36.0	41.0	33.0	41.0
35	37.62556840955896	40.0	36.0	41.0	33.0	41.0
36	37.587342722745134	40.0	35.0	41.0	33.0	41.0
37	37.56301225967851	40.0	35.0	41.0	33.0	41.0
38	37.47877246571114	40.0	35.0	41.0	33.0	41.0
39	37.428114331522714	40.0	35.0	41.0	33.0	41.0
40	37.341117146503386	40.0	35.0	41.0	33.0	41.0
41	37.25793700156183	39.0	35.0	41.0	33.0	41.0
42	37.24701800942627	39.0	35.0	41.0	33.0	41.0
43	37.20908257430213	39.0	35.0	41.0	33.0	41.0
44	37.132815487461585	39.0	35.0	41.0	33.0	41.0
45	37.01169760381105	39.0	35.0	41.0	32.0	41.0
46	36.947819196233176	39.0	35.0	41.0	32.0	41.0
47	36.90760551570354	39.0	35.0	41.0	32.0	41.0
48	36.86943281134101	39.0	35.0	41.0	32.0	41.0
49	36.8549893344022	39.0	35.0	41.0	32.0	41.0
50	36.73377930735811	39.0	35.0	41.0	32.0	41.0
51	35.80917794271445	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	6.0
10	8.0
11	6.0
12	5.0
13	5.0
14	9.0
15	16.0
16	31.0
17	49.0
18	74.0
19	141.0
20	270.0
21	399.0
22	601.0
23	921.0
24	1220.0
25	1718.0
26	2293.0
27	2883.0
28	3399.0
29	4206.0
30	5267.0
31	6824.0
32	9267.0
33	13494.0
34	28067.0
35	36266.0
36	29848.0
37	45738.0
38	82549.0
39	158385.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.804983114723136	21.00754193676199	25.048260102371312	11.139214846143568
2	31.97606114635596	24.277019898365836	25.617245557536638	18.129673397741566
3	33.22253090259061	23.763320479329934	25.808212740667024	17.20593587741243
4	30.136879011117102	25.33943322598628	25.319622396373237	19.204065366523384
5	25.732194440989065	29.320027827304852	24.456469157302593	20.491308574403487
6	26.5962691139952	33.656987003174336	23.966266303621698	15.780477579208766
7	78.99130627081865	6.092981898430337	9.089715415129024	5.825996415621991
8	78.41932615536298	5.651384684846558	9.402081519260273	6.527207640530193
9	72.19918637383496	7.873883337249428	12.064795234343686	7.862135054571925
10	41.838168557909825	25.35878333863158	18.23817224364556	14.564875859813043
11	31.101389983091686	24.530874947593446	24.739579733982023	19.628155335332846
12	29.811612831888983	20.90664492082579	29.021713590689828	20.260028656595395
13	26.290583405896257	22.707357189257923	29.894772244566997	21.107287160278826
14	20.307943221240894	26.704307242931453	29.774985832953245	23.212763702874415
15	19.760150746591844	24.543775022690312	34.97348573850626	20.722588492211578
16	23.380695037617542	25.152842854049474	30.29974245921503	21.166719649117958
17	22.15564862038304	24.684063339368727	28.55224300055747	24.60804503969077
18	23.214145853777648	25.524871805503725	29.713940834727005	21.547041505991622
19	24.09687956397746	27.203033360515633	26.99548036654642	21.704606708960483
20	25.361547640438047	26.687491073608747	27.276517716870995	20.674443569082207
21	24.485494326270544	26.76281829783509	28.308063007652507	20.44362436824186
22	22.171773714254122	25.116446213597598	27.77869921171327	24.93308086043501
23	22.95338005003386	25.472810788148514	28.288482536523336	23.285326625294285
24	24.424679686528176	23.412714866875834	28.596471829461006	23.566133617134984
25	22.41595370715908	25.262954209340577	27.36681824254906	24.954273840951288
26	20.749540434824674	26.13094497657254	28.88534136823725	24.23417322036553
27	21.644252786185863	26.698778639318512	28.511930265879766	23.145038308615867
28	18.17873975480643	27.690011195422315	29.811612831888983	24.31963621788227
29	21.566621977120796	25.80959489157026	29.561904235371085	23.06187889593786
30	23.762859762362186	25.96209220789392	27.945939471004777	22.32910855873911
31	23.533883429392823	26.801748881609562	27.11872215541826	22.545645533579357
32	23.822522609685194	28.445817381008325	25.902659719054792	21.82900029025169
33	25.11183904392015	26.843674125674376	24.985832953241836	23.058653877163643
34	22.850409807742807	25.73242479947294	29.41355337175713	22.003612021027124
35	21.379801246700115	28.10281359852202	27.539356746969634	22.97802840780823
36	22.72486443403224	28.91367546175358	26.61930496238246	21.742155141831716
37	22.71081256651601	28.827521388785225	28.152340672554633	20.30932537214413
38	22.625119210515404	29.011347458915566	25.291979378308522	23.07155395226051
39	24.87410908856362	25.380437036115605	26.06529280866885	23.680161066651923
40	25.198223475372373	23.626257181425736	28.10880291910271	23.06671642409918
41	20.961930956955214	26.534533040317342	26.99985717774	25.503678824987446
42	20.883148355470784	25.748089176376276	28.232735783426165	25.13602668472677
43	21.89718640147798	24.76123343146605	28.581498528009284	24.760081639046685
44	21.738239047605884	24.316871916075797	27.861628265907406	26.083260770410917
45	23.583871220393178	22.944626427646703	27.4900600314209	25.981442320539223
46	24.514058778270744	25.394028186664087	27.56193187838915	22.52998115667602
47	20.549819629307127	25.38642635669629	29.839946925405314	24.223807088591265
48	20.582991250984783	24.007039755267144	29.682381722436457	25.727587271311613
49	22.758496772677642	22.45580572486904	30.4034037769577	24.382293725495614
50	21.211639553473116	22.732235905516166	29.51260751982235	26.54351702118837
51	20.22063735585318	22.626731719902512	27.889040925488246	29.26358999875606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	413.0
2	587.0
3	1054.0
4	1521.0
5	1064.5
6	608.0
7	598.5
8	589.0
9	615.5
10	642.0
11	638.0
12	634.0
13	632.5
14	631.0
15	693.0
16	755.0
17	791.5
18	828.0
19	833.5
20	839.0
21	1083.0
22	1327.0
23	1333.0
24	1339.0
25	1751.5
26	2632.0
27	3100.0
28	3505.0
29	3910.0
30	4699.5
31	5489.0
32	6509.5
33	7530.0
34	8654.5
35	9779.0
36	10606.5
37	11434.0
38	12465.5
39	13497.0
40	16296.5
41	19096.0
42	22912.0
43	26728.0
44	32430.5
45	38133.0
46	42674.0
47	47215.0
48	47068.0
49	46921.0
50	40872.0
51	34823.0
52	30640.5
53	26458.0
54	24022.0
55	21586.0
56	20874.5
57	20163.0
58	19900.0
59	19637.0
60	19297.0
61	18957.0
62	17553.5
63	16150.0
64	13312.0
65	10474.0
66	8868.0
67	7262.0
68	6009.0
69	4756.0
70	4037.0
71	3318.0
72	2784.0
73	2250.0
74	1729.0
75	975.0
76	742.0
77	598.0
78	454.0
79	298.5
80	143.0
81	109.0
82	75.0
83	53.5
84	32.0
85	33.0
86	34.0
87	24.0
88	14.0
89	23.5
90	33.0
91	16.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.05804934777196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.98060321708485	23.1342223453724
2	11.864845569928816	7.844573007088005
3	4.425759591894765	4.389209369706967
4	2.223306755292781	2.9399273772681407
5	1.4029373289237008	2.3189185725695545
6	0.9999766622042461	1.9834366707460953
7	0.7474460714749107	1.7296376380931118
8	0.6418254606229968	1.6973998199945197
9	0.55709338189333	1.6574778458952184
>10	7.0069290524496255	45.95047410864724
>50	0.09787495398101813	2.213678317081476
>100	0.047881272451145554	2.8053891774412145
>500	0.00140827271915134	0.29888148886778293
>1k	0.00211240907872701	1.0367742612282749
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1701	0.39183978106729694	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	1649	0.37986113990592163	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	1104	0.25431576619535323	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	712	0.16401524051729302	No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	572	0.13176505277512865	TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	491	0.11310601558144784	TruSeq Adapter, Index 21 (95% over 22bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	471	0.1084988459039958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.910754516178076E-4	0.0	0.0	0.2626086716147669	0.0
2	6.910754516178076E-4	0.0	0.0	1.3206451880416303	0.0
3	6.910754516178076E-4	0.0	0.0	1.7986390420772806	0.0
4	6.910754516178076E-4	0.0	0.0	2.475432267694987	0.0
5	6.910754516178076E-4	0.0	0.0	4.393397004418276	0.0
6	6.910754516178076E-4	0.0	0.0	5.399833220457676	0.0
7	6.910754516178076E-4	0.0	0.0	6.4705394534975325	0.0
8	6.910754516178076E-4	0.0	0.0	8.235546156929413	0.0
9	6.910754516178076E-4	0.0	0.0	8.916485835256827	0.0
10	6.910754516178076E-4	0.0	0.0	10.365901415783242	0.0
11	6.910754516178076E-4	0.0	0.0	12.311509170571243	0.0
12	6.910754516178076E-4	0.0	0.0	13.898448765969603	0.0
13	6.910754516178076E-4	0.0	0.0	14.548981124425831	0.0
14	6.910754516178076E-4	0.0	0.0	14.810438003621236	0.0
15	6.910754516178076E-4	0.0	0.0	15.325519573560374	0.0
16	6.910754516178076E-4	0.0	0.0	16.33587188382561	0.0
17	6.910754516178076E-4	0.0	0.0	17.463246303898128	0.0
18	9.214339354904102E-4	0.0	0.0	18.617572666583737	0.0
19	9.214339354904102E-4	0.0	0.0	19.38697000271823	0.0
20	9.214339354904102E-4	0.0	0.0	20.12941539623963	0.0
21	9.214339354904102E-4	0.0	0.0	21.057529727762343	0.0
22	9.214339354904102E-4	0.0	0.0	22.036553284220904	0.0
23	9.214339354904102E-4	0.0	0.0	22.916292334130375	0.0
24	9.214339354904102E-4	0.0	0.0	23.588939107038374	0.0
25	9.214339354904102E-4	0.0	0.0	24.216665975591216	0.0
26	0.0011517924193630126	0.0	0.0	24.808917637627676	0.0
27	0.0011517924193630126	0.0	0.0	25.38711743214791	0.0
28	0.0011517924193630126	0.0	0.0	25.994803112603833	0.0
29	0.0011517924193630126	0.0	0.0	26.598342340350055	0.0
30	0.0011517924193630126	0.0	0.0	27.25647652877408	0.0
31	0.0013821509032356152	0.0	0.0	27.866696152552603	0.0
32	0.0013821509032356152	0.0	0.0	28.480140795105342	0.0
33	0.0013821509032356152	0.0	0.0	29.065251344141753	0.0
34	0.0013821509032356152	0.0	0.0	29.63515823324257	0.0
35	0.0013821509032356152	0.0	0.0	30.224645593472562	0.0
36	0.0013821509032356152	0.0	0.0	30.818049047928387	0.0
37	0.0013821509032356152	0.0	0.0	31.40523282331965	0.0
38	0.0013821509032356152	0.0	0.0	31.987118353581845	0.0
39	0.0013821509032356152	0.0	0.0	32.62774529723155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGTA	35	1.2095916E-7	45.000004	10
ATTGTCG	30	2.1623691E-6	45.000004	1
TCGTTTG	20	7.028431E-4	45.0	30
TTGGCTA	25	3.8867805E-5	45.0	40
GTCGACG	25	3.8867805E-5	45.0	1
CACGAAG	20	7.028431E-4	45.0	1
TCGATAG	20	7.028431E-4	45.0	1
GTATGCG	25	3.8867805E-5	45.0	1
ATTGGCC	20	7.028431E-4	45.0	12
TTACGGA	20	7.028431E-4	45.0	26
TCGAGAG	25	3.8867805E-5	45.0	1
CCGCATC	20	7.028431E-4	45.0	20
CCCATAT	20	7.028431E-4	45.0	14
CGAATAT	65	0.0	44.999996	14
CGCTAGG	60	3.6379788E-12	41.250004	2
TAGGGCG	110	0.0	40.909092	5
TACTCAC	55	6.002665E-11	40.909092	30
GTGCACG	50	1.0786607E-9	40.5	1
CATAAGG	45	1.9235813E-8	40.0	2
CGTAAGG	125	0.0	39.6	2
>>END_MODULE