##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551008_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 553177 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965549905364828 33.0 31.0 34.0 30.0 34.0 2 32.01175573098664 33.0 31.0 34.0 30.0 34.0 3 32.04007758818606 33.0 31.0 34.0 30.0 34.0 4 35.73177662845708 37.0 35.0 37.0 33.0 37.0 5 33.659195881245964 37.0 35.0 37.0 32.0 37.0 6 34.63064263337051 37.0 35.0 37.0 31.0 37.0 7 35.734772053068006 37.0 35.0 37.0 33.0 37.0 8 35.75078862642518 37.0 35.0 37.0 35.0 37.0 9 37.63070771199815 39.0 37.0 39.0 35.0 39.0 10 37.195931862676865 39.0 37.0 39.0 34.0 39.0 11 37.16117445230008 39.0 37.0 39.0 34.0 39.0 12 37.05948548113895 39.0 37.0 39.0 34.0 39.0 13 36.982784895250525 39.0 37.0 39.0 33.0 39.0 14 38.05709745705263 40.0 37.0 41.0 33.0 41.0 15 38.15013097073812 40.0 37.0 41.0 33.0 41.0 16 38.16388606178493 40.0 37.0 41.0 34.0 41.0 17 38.129304002154825 40.0 37.0 41.0 34.0 41.0 18 38.132679052093636 40.0 37.0 41.0 34.0 41.0 19 38.042642770758725 40.0 37.0 41.0 34.0 41.0 20 38.0983771921103 40.0 36.0 41.0 34.0 41.0 21 38.0176507700067 40.0 36.0 41.0 34.0 41.0 22 38.068666990854645 40.0 36.0 41.0 34.0 41.0 23 38.066607975385814 40.0 36.0 41.0 34.0 41.0 24 38.01336100380168 40.0 36.0 41.0 34.0 41.0 25 37.83031290165715 40.0 36.0 41.0 33.0 41.0 26 37.92309694727004 40.0 36.0 41.0 34.0 41.0 27 37.92355430540315 40.0 36.0 41.0 34.0 41.0 28 37.83605247506675 40.0 36.0 41.0 34.0 41.0 29 37.83432246821542 40.0 36.0 41.0 34.0 41.0 30 37.69503070445807 40.0 36.0 41.0 33.0 41.0 31 37.61373845984197 40.0 36.0 41.0 33.0 41.0 32 37.53687698512411 40.0 35.0 41.0 33.0 41.0 33 37.48840425397296 40.0 35.0 41.0 33.0 41.0 34 37.36253857264492 40.0 35.0 41.0 33.0 41.0 35 37.29429640060957 40.0 35.0 41.0 33.0 41.0 36 37.20332913335153 39.0 35.0 41.0 33.0 41.0 37 37.12052923386186 39.0 35.0 41.0 32.0 41.0 38 37.042295684744666 39.0 35.0 41.0 32.0 41.0 39 36.971504599793555 39.0 35.0 41.0 32.0 41.0 40 36.86289921670641 39.0 35.0 41.0 31.0 41.0 41 36.74732138176388 39.0 35.0 41.0 31.0 41.0 42 36.80940820026863 39.0 35.0 41.0 32.0 41.0 43 36.74215486182542 39.0 35.0 41.0 32.0 41.0 44 36.61625844892322 39.0 35.0 41.0 31.0 41.0 45 36.5439072846485 39.0 35.0 41.0 31.0 41.0 46 36.41791325380484 39.0 35.0 40.0 31.0 41.0 47 36.30300247479559 38.0 35.0 40.0 31.0 41.0 48 36.25891712779092 38.0 35.0 40.0 31.0 41.0 49 36.187048630004504 38.0 35.0 40.0 31.0 41.0 50 36.02374646812864 38.0 35.0 40.0 30.0 41.0 51 34.74956478667768 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 9.0 10 8.0 11 12.0 12 10.0 13 10.0 14 16.0 15 23.0 16 31.0 17 61.0 18 136.0 19 207.0 20 409.0 21 643.0 22 1042.0 23 1378.0 24 1897.0 25 2690.0 26 3496.0 27 4323.0 28 5114.0 29 6321.0 30 8058.0 31 10559.0 32 14051.0 33 21117.0 34 38366.0 35 50217.0 36 43795.0 37 66459.0 38 111539.0 39 161090.0 40 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18115720646376 19.256765917599612 23.316949186245996 13.245127689690642 2 31.1478966045226 25.245084303938885 25.6932229648015 17.91379612673701 3 32.67453274449227 24.996339327195454 26.000538706417657 16.328589221894617 4 28.891656739163054 26.039224335068162 26.304600516651995 18.764518409116793 5 24.514215160789405 34.326806790593245 22.31907689582177 18.83990115279558 6 25.25412300222171 35.740459202027566 24.113800826860118 14.89161696889061 7 79.42448800293576 5.740658053389783 9.812772403769499 5.022081539904949 8 79.91565086762465 6.06840125312513 8.327352727969528 5.688595151280693 9 74.27152611189547 6.730757063290774 11.126637586161392 7.871079238652366 10 42.032658624635516 23.358346424381345 18.221654190250135 16.387340760733 11 32.70128729140944 23.83775898130255 24.371222953955062 19.089730773332946 12 28.163318431532762 21.246183409650076 29.379023350573142 21.211474808244017 13 25.489309931540898 24.509876585613647 30.759413352326654 19.241400130518805 14 21.044981985874323 27.22564387167218 29.221750000451934 22.507624142001564 15 19.122812409048098 23.89181039703386 36.50874855606795 20.47662863785009 16 22.662005108672272 23.772680353666185 31.517940912221587 22.04737362543996 17 21.824117777854106 23.297425597955083 29.922249117371113 24.9562075068197 18 23.087004701930848 23.55755933453488 30.786529447175138 22.568906516359142 19 23.84860541924194 26.29230788698735 27.610511644554997 22.248575049215713 20 25.234960961862118 25.194467593555046 28.403567031890336 21.167004412692503 21 23.89343736272477 26.736288746639865 28.632246098445886 20.73802779218948 22 21.547533610399565 24.272881916637893 28.75318388147012 25.426400591492417 23 21.40833765684401 27.32470800485197 27.954343727233777 23.312610611070237 24 22.886887921949032 23.90139141721366 29.86892079750243 23.342799863334882 25 20.584189147415746 26.216202047445936 28.200377094492357 24.999231710645958 26 20.155212526912724 26.281822996979265 29.8461432778297 23.71682119827831 27 21.486432010007647 26.677537207801482 28.37337777962569 23.462653002565183 28 20.034274743888485 25.47231717876918 30.908371100027658 23.585036977314676 29 21.66865216738946 24.36561896101971 29.517676982231727 24.4480518893591 30 23.622457188205583 26.142627043423715 28.129513699954988 22.105402068415714 31 24.999412484611618 26.577026882896433 25.03394031205202 23.38962032043993 32 24.26944721129042 27.33446979899743 25.942691037407556 22.453391952304596 33 24.273966560431834 26.396976013102496 24.71577813249647 24.6132792939692 34 23.130932775585393 25.722869895169175 27.773750535542874 23.372446793702558 35 20.807264221035943 27.91168107133883 26.726346178528754 24.554708529096473 36 23.944596395005576 27.090605719326728 26.998591770807533 21.966206114860164 37 21.827190935270266 28.421825202421648 26.79576338134087 22.955220480967213 38 23.60275282594902 27.65606668390045 23.867767459601538 24.873413030548992 39 22.82813638311065 25.167170724740906 26.14750794049644 25.857184951652002 40 23.263801640342965 25.746189736738874 27.547059982609547 23.442948640308618 41 19.64958774497132 27.179908058361068 26.840053002926727 26.330451193740885 42 22.125467978603595 25.856461855789377 27.226366967534805 24.791703198072227 43 23.08212380485812 24.086865505977293 27.01721148927016 25.813799199894426 44 21.98066803211269 24.866001297957073 27.54362527726207 25.609705392668168 45 22.34492757291066 24.41967037675102 26.91778580815905 26.317616242179266 46 24.05541083595305 25.629952076821706 26.527314042340876 23.787323044884367 47 20.334540300844033 25.324986396759087 29.66356157251657 24.67691172988031 48 21.33440110489048 24.716139680427784 28.045453805924687 25.90400540875705 49 22.801020288262166 22.405667625371265 29.804565265728687 24.98874682063788 50 21.213101773934927 22.997160040999535 29.597217527120613 26.19252065794492 51 20.47572476802181 23.236504771528825 27.361947441777225 28.92582301867214 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 204.0 1 524.5 2 845.0 3 1686.0 4 2527.0 5 1756.5 6 986.0 7 941.5 8 897.0 9 939.5 10 982.0 11 1071.5 12 1161.0 13 1103.5 14 1046.0 15 1036.5 16 1027.0 17 1085.0 18 1143.0 19 1185.5 20 1228.0 21 1316.5 22 1405.0 23 1551.5 24 1698.0 25 1971.5 26 3086.5 27 3928.0 28 4466.0 29 5004.0 30 6051.5 31 7099.0 32 8007.0 33 8915.0 34 10156.5 35 11398.0 36 12919.5 37 14441.0 38 15845.0 39 17249.0 40 19648.5 41 22048.0 42 25633.5 43 29219.0 44 33486.5 45 37754.0 46 46553.0 47 55352.0 48 57616.0 49 59880.0 50 59159.0 51 58438.0 52 51261.5 53 44085.0 54 38527.5 55 32970.0 56 29716.5 57 26463.0 58 25157.0 59 23851.0 60 22615.5 61 21380.0 62 19344.5 63 17309.0 64 15059.5 65 12810.0 66 10613.0 67 8416.0 68 7251.5 69 6087.0 70 5405.0 71 4723.0 72 3730.0 73 2737.0 74 2357.5 75 1451.0 76 924.0 77 794.0 78 664.0 79 536.5 80 409.0 81 245.5 82 82.0 83 79.5 84 77.0 85 56.0 86 35.0 87 29.0 88 23.0 89 23.0 90 23.0 91 15.5 92 8.0 93 5.5 94 3.0 95 1.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 553177.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.756592721535032 #Duplication Level Percentage of deduplicated Percentage of total 1 71.30002343885214 22.642458053835288 2 11.757389202596611 7.467492407508683 3 4.415821070919875 4.206942938411254 4 2.136851687708683 2.7143651501155768 5 1.253810072649106 1.9908367913637952 6 0.9019123004756937 1.7184996958049599 7 0.6537990563300398 1.4533701247517912 8 0.5350772751259858 1.359378488057974 9 0.43735336843068834 1.2499967516980344 >10 6.358912401304911 46.621139797685274 >50 0.206682977802023 4.091398933059169 >100 0.03778691560923397 2.1868793657217047 >500 5.72529024382333E-4 0.09818048366226477 >1k 0.004007703170676331 2.199061018324245 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC 2350 0.42481881929292076 No Hit GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC 2073 0.374744430806053 No Hit GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC 2046 0.36986353373332587 RNA PCR Primer, Index 33 (95% over 24bp) GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG 1791 0.32376617249090256 No Hit CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC 1502 0.2715224964161561 Illumina Paired End PCR Primer 2 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.22289429965454094 No Hit CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT 1100 0.19885136222221822 TruSeq Adapter, Index 1 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.42321896969686E-4 0.0 0.0 0.21765185465050066 0.0 2 5.42321896969686E-4 0.0 0.0 1.0924170744626043 0.0 3 5.42321896969686E-4 0.0 0.0 1.4552304235353242 0.0 4 5.42321896969686E-4 0.0 0.0 2.038949558640363 0.0 5 5.42321896969686E-4 0.0 0.0 3.6348221274564922 0.0 6 5.42321896969686E-4 0.0 0.0 4.6601720606605115 0.0 7 5.42321896969686E-4 0.0 0.0 5.693295274387764 0.0 8 5.42321896969686E-4 0.0 0.0 7.466687877478637 0.0 9 5.42321896969686E-4 0.0 0.0 8.175502596818017 0.0 10 5.42321896969686E-4 0.0 0.0 9.850915710523033 0.0 11 5.42321896969686E-4 0.0 0.0 11.448957566927042 0.0 12 5.42321896969686E-4 0.0 0.0 13.248200847106803 0.0 13 5.42321896969686E-4 0.0 0.0 13.826677537207802 0.0 14 5.42321896969686E-4 0.0 0.0 14.089703657238099 0.0 15 5.42321896969686E-4 0.0 0.0 14.542542441207788 0.0 16 5.42321896969686E-4 0.0 0.0 15.33740556819969 0.0 17 5.42321896969686E-4 0.0 0.0 16.28176876478957 0.0 18 5.42321896969686E-4 0.0 0.0 17.337488724223892 0.0 19 5.42321896969686E-4 0.0 0.0 17.993517445591557 0.0 20 5.42321896969686E-4 0.0 0.0 18.639242050916796 0.0 21 5.42321896969686E-4 0.0 0.0 19.376619056829913 0.0 22 5.42321896969686E-4 0.0 0.0 20.136773582415756 0.0 23 5.42321896969686E-4 0.0 0.0 20.87686219781372 0.0 24 5.42321896969686E-4 0.0 0.0 21.476670215862192 0.0 25 5.42321896969686E-4 0.0 0.0 22.015195859553092 0.0 26 5.42321896969686E-4 0.0 0.0 22.50726259407025 0.0 27 5.42321896969686E-4 0.0 0.0 23.002944807900544 0.0 28 5.42321896969686E-4 0.0 0.0 23.520681445540937 0.0 29 7.23095862626248E-4 0.0 0.0 24.075295972175272 0.0 30 7.23095862626248E-4 0.0 0.0 24.685408106266166 0.0 31 7.23095862626248E-4 0.0 0.0 25.23062238668636 0.0 32 7.23095862626248E-4 0.0 0.0 25.747816702429784 0.0 33 7.23095862626248E-4 0.0 0.0 26.289415503536844 0.0 34 7.23095862626248E-4 0.0 0.0 26.803536661864104 0.0 35 7.23095862626248E-4 0.0 0.0 27.346220106765106 0.0 36 7.23095862626248E-4 0.0 0.0 27.85871429940146 0.0 37 9.0386982828281E-4 0.0 0.0 28.359819732201448 0.0 38 9.0386982828281E-4 0.0 0.0 28.904130142793356 0.0 39 9.0386982828281E-4 0.0 0.0 29.426205355609508 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATAT 55 1.8189894E-12 45.000004 14 CGTAAGG 55 1.8189894E-12 45.000004 2 TCACGGA 20 7.0301106E-4 45.0 25 AACGTTC 20 7.0301106E-4 45.0 21 TTAGGCG 20 7.0301106E-4 45.0 1 GACGTAG 20 7.0301106E-4 45.0 1 TTCGTAT 20 7.0301106E-4 45.0 40 CGAATAA 20 7.0301106E-4 45.0 36 TTTGCGT 20 7.0301106E-4 45.0 12 CCAACGA 20 7.0301106E-4 45.0 24 GCCCGTC 25 3.888172E-5 45.0 33 ATCGCAA 20 7.0301106E-4 45.0 19 GTACGAG 45 3.8380676E-10 45.0 1 ACGATAG 30 2.1634532E-6 44.999996 1 TCGTGCG 30 2.1634532E-6 44.999996 1 TCGTAAG 30 2.1634532E-6 44.999996 1 AATCGTT 30 2.1634532E-6 44.999996 22 TAATCGT 30 2.1634532E-6 44.999996 21 GTTACGG 70 0.0 41.785713 2 TAGCGCG 40 3.453879E-7 39.375 1 >>END_MODULE