FastQCFastQC Report
Sat 18 Jun 2016
SRR3551006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323449
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC54361.680635896230936No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC52011.6079814746683403No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG49361.5260520205658388No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC24400.754369313245674TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT11260.3481228879977987No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC9650.29834688003363746TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT8960.27701430519185405No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT8520.2634109241333255No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC8300.25660923360406124No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA8090.25011671082612713No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT7280.22507412296838142TruSeq Adapter, Index 16 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC6750.2086882321478811No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG6340.1960123543433432No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.1932298445813714No Hit
GAATATGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC5480.16942392772894646No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC4230.13077795881267215TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT3390.10480786770093585No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG207.02576E-445.0000042
TTGGACG207.02576E-445.0000041
GCGAAAG207.02576E-445.0000041
GCGTTAG207.02576E-445.0000041
GCGATCT207.02576E-445.0000049
TGTAGCG207.02576E-445.0000041
CGGTAGC207.02576E-445.00000433
CCTATCG207.02576E-445.00000440
TCGACAG207.02576E-445.0000041
TAATACG1350.045.04
CGGCGAT502.1827873E-1145.031
CGAAAGG253.884569E-545.02
GCGAACG253.884569E-545.01
AGGGTCA453.8380676E-1045.06
CCTCGTG351.2083183E-745.015
GTAGCAT351.2083183E-745.029
TCGTCCC253.884569E-545.038
AATCTCG253.884569E-545.025
CCGACGG253.884569E-545.02
TAAGGGT700.045.04
Produced by FastQC (version 0.11.5)