##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323449
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85821566923997	33.0	31.0	34.0	30.0	34.0
2	31.96411799078062	33.0	31.0	34.0	30.0	34.0
3	31.957922269043962	33.0	31.0	34.0	30.0	34.0
4	35.62978398449214	37.0	35.0	37.0	33.0	37.0
5	31.798666868656266	37.0	35.0	37.0	0.0	37.0
6	33.64471987855891	37.0	35.0	37.0	17.0	37.0
7	35.311066041323365	37.0	35.0	37.0	32.0	37.0
8	35.48763792746306	37.0	35.0	37.0	33.0	37.0
9	37.42162442919904	39.0	37.0	39.0	34.0	39.0
10	37.084350237595416	39.0	37.0	39.0	33.0	39.0
11	37.09109936960696	39.0	37.0	39.0	34.0	39.0
12	36.998794245769815	39.0	37.0	39.0	33.0	39.0
13	36.855362050895195	39.0	37.0	39.0	33.0	39.0
14	38.02300517237648	40.0	37.0	41.0	33.0	41.0
15	38.095854369622415	40.0	37.0	41.0	33.0	41.0
16	38.17221880420097	40.0	37.0	41.0	34.0	41.0
17	38.037254714035285	40.0	37.0	41.0	33.0	41.0
18	38.019029275094375	40.0	37.0	41.0	33.0	41.0
19	37.940562499806774	40.0	37.0	41.0	33.0	41.0
20	37.85027005803079	40.0	36.0	41.0	33.0	41.0
21	37.94470534767459	40.0	36.0	41.0	33.0	41.0
22	37.99646002924727	40.0	37.0	41.0	33.0	41.0
23	37.95122569555015	40.0	36.0	41.0	33.0	41.0
24	37.90745063363931	40.0	36.0	41.0	33.0	41.0
25	37.782398461581266	40.0	36.0	41.0	33.0	41.0
26	37.765718861397005	40.0	36.0	41.0	33.0	41.0
27	37.70271974870845	40.0	36.0	41.0	33.0	41.0
28	37.63316318801419	40.0	36.0	41.0	33.0	41.0
29	37.62602450463597	40.0	36.0	41.0	33.0	41.0
30	37.52525745944492	40.0	36.0	41.0	33.0	41.0
31	37.52165874681943	40.0	36.0	41.0	33.0	41.0
32	37.37853571969615	40.0	36.0	41.0	33.0	41.0
33	37.346901675380046	40.0	36.0	41.0	33.0	41.0
34	37.2650093214077	40.0	36.0	41.0	32.0	41.0
35	37.21658746819437	40.0	36.0	41.0	32.0	41.0
36	37.113390364477866	40.0	35.0	41.0	32.0	41.0
37	37.01105583878757	40.0	35.0	41.0	32.0	41.0
38	36.954867691660816	40.0	35.0	41.0	31.0	41.0
39	36.863613738178195	39.0	35.0	41.0	31.0	41.0
40	36.87900720051693	39.0	35.0	41.0	31.0	41.0
41	36.80553348441331	39.0	35.0	41.0	31.0	41.0
42	36.772038250234196	39.0	35.0	41.0	31.0	41.0
43	36.688210506138525	39.0	35.0	41.0	31.0	41.0
44	36.73239057780361	39.0	35.0	41.0	31.0	41.0
45	36.71223902377191	39.0	35.0	41.0	31.0	41.0
46	36.60601207609237	39.0	35.0	41.0	31.0	41.0
47	36.43416118151548	39.0	35.0	41.0	31.0	41.0
48	36.42778614248305	39.0	35.0	41.0	31.0	41.0
49	36.46063212438437	39.0	35.0	41.0	31.0	41.0
50	36.34610711425913	39.0	35.0	41.0	31.0	41.0
51	35.42180683817232	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	10.0
11	5.0
12	8.0
13	5.0
14	4.0
15	11.0
16	16.0
17	45.0
18	87.0
19	166.0
20	322.0
21	703.0
22	1183.0
23	1678.0
24	2004.0
25	2310.0
26	2514.0
27	2694.0
28	3033.0
29	3626.0
30	4680.0
31	6286.0
32	8775.0
33	12502.0
34	20128.0
35	26013.0
36	25172.0
37	37362.0
38	61262.0
39	100772.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.17745919758602	18.78286839656329	21.42099681866384	10.618675587186852
2	30.54546466367186	30.71859860441677	22.830492596978193	15.905444134933171
3	29.787076169658892	31.084962389743048	23.788912626101798	15.339048814496259
4	28.03069417435206	22.365813466728913	31.85077090978794	17.752721449131084
5	26.683341114055075	35.336018970533225	20.30613790736716	17.674502008044545
6	22.717028032240012	38.99780181728804	23.85940287340508	14.425767277066864
7	72.15851648946202	6.08071133316226	16.58437651685428	5.176395660521442
8	71.04674925567865	11.943150233885405	10.593632999329106	6.416467511106852
9	66.00731490899648	6.944526030378824	11.751466228060684	15.296692832564021
10	40.213140247767036	22.828946758221544	20.510497790996418	16.447415203015005
11	32.64718703721452	22.610365158031094	24.215564122937465	20.52688368181692
12	26.049856391579507	20.396105723004247	29.644859004047007	23.909178881369243
13	24.94179917081209	23.452538112654544	33.40279302146551	18.202869695067847
14	20.287587842287348	30.367074871154337	27.252828112005297	22.092509174553022
15	17.99912814694125	24.69910248601789	36.16026019557952	21.141509171461344
16	20.298099545832574	24.215254955186136	28.404168817958936	27.082476681022357
17	19.898963978865293	26.113235780602196	29.591991318569544	24.39580892196297
18	20.093121326700654	24.62613889670396	30.17879171059425	25.101948066001135
19	21.171498443340372	26.456102816827382	27.27910737086836	25.09329136896389
20	23.75088499268818	26.139205871713934	30.916156797516763	19.19375233808112
21	21.798490643038008	30.1086106310423	28.13890288731763	19.95399583860207
22	19.51126761869723	23.996364187244357	29.90455991516437	26.587808278894045
23	21.2178736060399	27.755225707916857	28.24927577454251	22.777624911500734
24	22.314491620008102	25.214794295236654	27.352380127933618	25.11833395682163
25	18.7624633249755	31.404023509115813	26.21649780954648	23.61701535636221
26	18.603241933040447	24.662929859112257	30.46415354507202	26.269674662775277
27	23.644222118479266	25.835603139907683	27.364746837986825	23.155427903626226
28	18.00098315344923	26.75383136135836	30.98633787706872	24.258847608123695
29	20.511116126499076	23.66833720308302	28.85215288963639	26.968393780781515
30	19.900509817621945	26.76217889064427	30.3543989933498	22.98291229838398
31	24.05170521473246	24.72754591914027	24.055106059997094	27.16564280613018
32	23.47788986826362	28.620277076138745	25.578066403049633	22.323766652548006
33	21.584546559117513	23.182634665743286	26.4112734928845	28.821545282254696
34	20.77236287637309	23.27383915238569	26.49814963100829	29.455648340232926
35	19.744998438702858	22.452998772604026	31.10072994506089	26.70127284363223
36	22.386218538316704	23.50355079162403	28.798666868656262	25.311563801403004
37	19.358847917291445	26.13487752319531	32.24186811522064	22.264406444292607
38	21.0079487028867	25.22932517954917	26.51453552182879	27.248190595735338
39	21.23456866461173	25.156979925737904	27.845193523553945	25.76325788609642
40	19.70418829552727	21.54868309996321	30.890805041907687	27.85632356260183
41	18.07703842027646	23.591972768504462	29.1563739569453	29.174614854273777
42	19.742525096692216	22.22112295910638	33.68289900417067	24.35345294003073
43	22.067466586695275	22.63200690062421	30.118194831333533	25.18233168134698
44	21.145219184477305	22.29439571617164	29.22253585572996	27.337849243621097
45	20.437534201682492	21.58392822361485	27.414213678199655	30.564323896503005
46	24.79185281141695	24.564305346437923	26.940568683161796	23.703273158983333
47	17.88813692421371	22.639117758904803	35.65075174138736	23.821993575494126
48	20.553162940679982	22.91984207711262	29.05960445077895	27.46739053142845
49	20.010264369344164	20.742064436742734	35.01448450914982	24.233186684763286
50	21.21261775426729	20.45577509901097	31.144322597998446	27.18728454872329
51	19.47138497877563	20.748866127272	27.522422391165225	32.25732650278715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	230.0
2	376.0
3	803.5
4	1231.0
5	865.0
6	499.0
7	469.5
8	440.0
9	454.0
10	468.0
11	461.0
12	454.0
13	469.0
14	484.0
15	533.5
16	583.0
17	559.0
18	535.0
19	634.0
20	733.0
21	774.5
22	816.0
23	942.0
24	1068.0
25	1409.0
26	1902.0
27	2054.0
28	2466.0
29	2878.0
30	3606.0
31	4334.0
32	4792.0
33	5250.0
34	5969.5
35	6689.0
36	7009.0
37	7329.0
38	8379.5
39	9430.0
40	11465.0
41	13500.0
42	15088.5
43	16677.0
44	20432.0
45	24187.0
46	35633.5
47	47080.0
48	41218.0
49	35356.0
50	34903.5
51	34451.0
52	29658.0
53	24865.0
54	21678.0
55	18491.0
56	17028.0
57	15565.0
58	14120.0
59	12675.0
60	11192.5
61	9710.0
62	8731.5
63	7753.0
64	6595.5
65	5438.0
66	4581.0
67	3724.0
68	3032.5
69	2341.0
70	2045.5
71	1750.0
72	1363.0
73	976.0
74	844.0
75	545.5
76	379.0
77	312.5
78	246.0
79	140.0
80	34.0
81	30.0
82	26.0
83	22.5
84	19.0
85	10.5
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	323449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.81749529682869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.28920933633465	22.410808062193123
2	12.502564585174591	8.206057089445281
3	4.799124023464839	4.724856902068647
4	2.4094472704507846	3.1628809786390133
5	1.5429388917357127	2.5317694911415405
6	1.1564838055505804	2.2771741109708827
7	0.9713315609990992	2.2313668254324437
8	0.7892015042214149	2.0719693322429125
9	0.707593376805501	2.089929808384349
>10	6.691781262208477	37.49624954800185
>50	0.09255438031244168	2.1398993620258437
>100	0.032841876885059955	1.9364260252520187
>500	0.009952083904563622	2.469767853424821
>1k	0.0029856251713690865	2.776774172152583
>5k	0.0019904167809127243	3.474070438624679
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	5436	1.680635896230936	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	5201	1.6079814746683403	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	4936	1.5260520205658388	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2440	0.754369313245674	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	1126	0.3481228879977987	No Hit
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	965	0.29834688003363746	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	896	0.27701430519185405	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGACCTTT	852	0.2634109241333255	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	830	0.25660923360406124	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTA	809	0.25011671082612713	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	728	0.22507412296838142	TruSeq Adapter, Index 16 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	675	0.2086882321478811	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	634	0.1960123543433432	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.1932298445813714	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	548	0.16942392772894646	No Hit
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	423	0.13077795881267215	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	339	0.10480786770093585	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.183355026603885E-4	0.0	0.0	0.23342165225429665	0.0
2	6.183355026603885E-4	0.0	0.0	1.556659627947528	0.0
3	6.183355026603885E-4	0.0	0.0	1.9406459750996292	0.0
4	6.183355026603885E-4	0.0	0.0	2.7132561856737847	0.0
5	6.183355026603885E-4	0.0	0.0	5.709091696063367	0.0
6	6.183355026603885E-4	0.0	0.0	6.632575769286658	0.0
7	6.183355026603885E-4	0.0	0.0	7.879140142650001	0.0
8	6.183355026603885E-4	0.0	0.0	9.45496817118	0.0
9	9.275032539905828E-4	0.0	0.0	9.928922333969188	0.0
10	0.001236671005320777	0.0	0.0	12.728436322264097	0.0
11	0.001236671005320777	0.0	0.0	14.284477614708965	0.0
12	0.001236671005320777	0.0	0.0	17.512188938596193	0.0
13	0.001236671005320777	0.0	0.0	18.129596938002592	0.0
14	0.001236671005320777	0.0	0.0	18.424233805020265	0.0
15	0.001236671005320777	0.0	0.0	19.109040374216647	0.0
16	0.001236671005320777	0.0	0.0	20.010573537095492	0.0
17	0.001236671005320777	0.0	0.0	21.019078741934585	0.0
18	0.001236671005320777	0.0	0.0	22.05788238640404	0.0
19	0.001236671005320777	0.0	0.0	23.028669125580848	0.0
20	0.001236671005320777	0.0	0.0	23.71656737229053	0.0
21	0.001236671005320777	0.0	0.0	24.443111587916487	0.0
22	0.001236671005320777	0.0	0.0	25.27662784550269	0.0
23	0.001236671005320777	0.0	0.0	26.02635964247841	0.0
24	0.001236671005320777	0.0	0.0	26.662317706964622	0.0
25	0.001236671005320777	0.0	0.0	27.221911336872274	0.0
26	0.001236671005320777	0.0	0.0	27.719362248762557	0.0
27	0.001236671005320777	0.0	0.0	28.288849246712775	0.0
28	0.001236671005320777	0.0	0.0	28.794647687888972	0.0
29	0.001236671005320777	0.0	0.0	29.30044612906517	0.0
30	0.001236671005320777	0.0	0.0	29.883845675825246	0.0
31	0.001236671005320777	0.0	0.0	30.439110957214275	0.0
32	0.001236671005320777	0.0	0.0	30.958512779449002	0.0
33	0.001236671005320777	0.0	0.0	31.471422078905793	0.0
34	0.001236671005320777	0.0	0.0	31.981548868600616	0.0
35	0.0015458387566509712	0.0	0.0	32.52970329170905	0.0
36	0.0018550065079811656	0.0	0.0	33.06147182399698	0.0
37	0.0018550065079811656	0.0	0.0	33.57809113646974	0.0
38	0.0018550065079811656	0.0	0.0	34.09625628769914	0.0
39	0.0018550065079811656	0.0	0.0	34.60452807088598	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.02576E-4	45.000004	2
TTGGACG	20	7.02576E-4	45.000004	1
GCGAAAG	20	7.02576E-4	45.000004	1
GCGTTAG	20	7.02576E-4	45.000004	1
GCGATCT	20	7.02576E-4	45.000004	9
TGTAGCG	20	7.02576E-4	45.000004	1
CGGTAGC	20	7.02576E-4	45.000004	33
CCTATCG	20	7.02576E-4	45.000004	40
TCGACAG	20	7.02576E-4	45.000004	1
TAATACG	135	0.0	45.0	4
CGGCGAT	50	2.1827873E-11	45.0	31
CGAAAGG	25	3.884569E-5	45.0	2
GCGAACG	25	3.884569E-5	45.0	1
AGGGTCA	45	3.8380676E-10	45.0	6
CCTCGTG	35	1.2083183E-7	45.0	15
GTAGCAT	35	1.2083183E-7	45.0	29
TCGTCCC	25	3.884569E-5	45.0	38
AATCTCG	25	3.884569E-5	45.0	25
CCGACGG	25	3.884569E-5	45.0	2
TAAGGGT	70	0.0	45.0	4
>>END_MODULE