FastQCFastQC Report
Sat 18 Jun 2016
SRR3551005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551280
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC43150.7827238426933681No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG31460.5706718908721521No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC31160.5652300101581773No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.3934479756203744No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC20460.3711362646930779TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC10840.19663328979828762TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT9710.17613553910898272No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT9330.1692424902046147TruSeq Adapter, Index 16 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT7000.12697721665941084No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT6820.12371208823102597No Hit
CGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG5970.10829342620809752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA207.030088E-445.00000434
CGTTATT1100.045.0000041
CCGACGG406.8048394E-945.0000042
CGTAAGC207.030088E-445.00000443
AATAGCG207.030088E-445.0000041
CGATAAC207.030088E-445.00000410
TCGGTAT207.030088E-445.00000438
TACGTTC207.030088E-445.00000419
CGAATGC453.8380676E-1045.045
CGTTTTT11600.041.7025871
CGTAAGG501.0786607E-940.52
TACGGGA3050.039.0983624
GTTGATC700.038.57142616
TAATACG1400.038.5714264
CGTTAGG700.038.5714262
TCAACGG356.242375E-638.5714262
CGATGAA3350.038.28358519
CCCTCGA301.1391514E-437.49999641
TACGCGG301.1391514E-437.4999962
GGAACGT301.1391514E-437.4999968