##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551280
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01232948773763	33.0	31.0	34.0	30.0	34.0
2	32.11464591496154	33.0	31.0	34.0	30.0	34.0
3	32.06323465389639	33.0	31.0	34.0	30.0	34.0
4	35.709470686402554	37.0	35.0	37.0	33.0	37.0
5	32.0839954288202	37.0	35.0	37.0	0.0	37.0
6	33.84367653461036	37.0	35.0	37.0	17.0	37.0
7	35.47947685386736	37.0	35.0	37.0	32.0	37.0
8	35.713363445073284	37.0	35.0	37.0	35.0	37.0
9	37.6644336816137	39.0	37.0	39.0	35.0	39.0
10	37.22933173704833	39.0	37.0	39.0	34.0	39.0
11	37.22653461036134	39.0	37.0	39.0	34.0	39.0
12	37.2349169206211	39.0	37.0	39.0	34.0	39.0
13	37.21769518212161	39.0	37.0	39.0	34.0	39.0
14	38.41995174865767	40.0	38.0	41.0	34.0	41.0
15	38.529277318241185	40.0	38.0	41.0	34.0	41.0
16	38.48955340298941	40.0	38.0	41.0	34.0	41.0
17	38.46639275867073	40.0	38.0	41.0	34.0	41.0
18	38.38949898418227	40.0	38.0	41.0	34.0	41.0
19	38.25966840806849	40.0	37.0	41.0	34.0	41.0
20	38.29074517486577	40.0	37.0	41.0	34.0	41.0
21	38.262169859236685	40.0	37.0	41.0	34.0	41.0
22	38.34203852851545	40.0	37.0	41.0	34.0	41.0
23	38.340893919605286	40.0	37.0	41.0	34.0	41.0
24	38.31805434624873	40.0	37.0	41.0	34.0	41.0
25	38.0817896531708	40.0	37.0	41.0	34.0	41.0
26	38.12136119576259	40.0	37.0	41.0	34.0	41.0
27	38.09371462777536	40.0	37.0	41.0	34.0	41.0
28	38.03057792773183	40.0	37.0	41.0	34.0	41.0
29	37.98997605572486	40.0	37.0	41.0	34.0	41.0
30	37.862403860107385	40.0	37.0	41.0	33.0	41.0
31	37.859155057321146	40.0	37.0	41.0	33.0	41.0
32	37.7597355245973	40.0	37.0	41.0	33.0	41.0
33	37.68780293135975	40.0	37.0	41.0	33.0	41.0
34	37.57299738789726	40.0	37.0	41.0	33.0	41.0
35	37.55974640835873	40.0	37.0	41.0	33.0	41.0
36	37.489716659410824	40.0	37.0	41.0	33.0	41.0
37	37.45929836017994	40.0	36.0	41.0	33.0	41.0
38	37.32643665650849	40.0	36.0	41.0	32.0	41.0
39	37.22876759541431	40.0	36.0	41.0	32.0	41.0
40	37.17337469162676	40.0	36.0	41.0	32.0	41.0
41	37.08147583804963	40.0	36.0	41.0	31.0	41.0
42	37.08624111159483	40.0	36.0	41.0	32.0	41.0
43	37.03476273400087	40.0	35.0	41.0	32.0	41.0
44	37.01265418662023	40.0	35.0	41.0	32.0	41.0
45	36.99264257727471	40.0	35.0	41.0	32.0	41.0
46	36.874258090262664	39.0	35.0	41.0	31.0	41.0
47	36.78150486141344	39.0	35.0	41.0	31.0	41.0
48	36.764410100130604	39.0	35.0	41.0	31.0	41.0
49	36.74329016107967	39.0	35.0	41.0	31.0	41.0
50	36.63190211870556	39.0	35.0	41.0	31.0	41.0
51	35.60195726309679	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	5.0
9	22.0
10	21.0
11	12.0
12	7.0
13	6.0
14	12.0
15	24.0
16	28.0
17	57.0
18	96.0
19	230.0
20	443.0
21	786.0
22	1288.0
23	1890.0
24	2635.0
25	3523.0
26	4301.0
27	4674.0
28	5228.0
29	5986.0
30	7224.0
31	9348.0
32	12604.0
33	18130.0
34	29057.0
35	38570.0
36	40138.0
37	62926.0
38	107979.0
39	193865.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.619612538093165	20.143847046872732	23.18730953417501	12.04923088085909
2	32.432883471194316	26.21335800319257	24.31450442606298	17.03925409955014
3	31.122115803221597	26.574880278624292	26.32509795385285	15.97790596430126
4	27.80619648817298	25.06911188506748	28.962777535916413	18.16191409084313
5	24.421709476128285	35.99894790306197	22.328943549557394	17.25039907125236
6	24.660789435495573	35.49648091713829	25.231461326367725	14.611268320998402
7	78.72152082426354	5.284428965317081	11.562726745029748	4.431323465389639
8	79.33046727615731	6.861123204179364	9.0084893339138	4.799920185749528
9	73.69177187636046	7.028733130169787	10.742635321433754	8.53685967203599
10	36.48889856334349	31.166739225076185	18.210165433173707	14.134196778406618
11	27.400413582934263	25.05804672761573	28.056523001015815	19.48501668843419
12	23.878065592802205	22.345994775794516	32.44068350021767	21.335256131185602
13	23.76523726599913	24.130206065883037	33.78591641271223	18.3186402554056
14	20.1939123494413	28.941010013060513	30.167609925990423	20.697467711507763
15	18.878972572921203	26.586126832099836	34.81624582789145	19.718654767087507
16	21.835002176752287	26.305507183282543	30.85491946016543	21.00457117979974
17	22.01730518067044	25.907887099114784	29.85343201277028	22.221375707444494
18	22.132854447830503	24.907488027862428	31.194855608765053	21.764801915542012
19	21.927695544913657	27.03580757509795	29.0090335219852	22.027463358003192
20	23.420766216804527	27.066826295167612	29.945037004788855	19.567370483239007
21	23.15103032941518	27.603214337541722	30.212414743868816	19.033340589174287
22	21.095631983746916	24.257183282542446	30.733202728196197	23.913982005514438
23	20.284610361340878	27.87911768974024	30.13876795820636	21.697503990712523
24	21.24292555507183	25.95269191699318	30.25594978958061	22.548432738354375
25	20.410317805833696	29.617617181831374	28.05688579306342	21.915179219271515
26	19.84091568712814	27.315701639820055	29.651538238281816	23.19184443476999
27	21.610615295312726	27.016398200551446	29.313597445943984	22.059389058191844
28	18.729683645334493	26.833732404585692	31.406544768538673	23.03003918154114
29	20.451676099259906	26.289544333188214	29.730264112610648	23.52851545494123
30	21.363191118850676	25.996952546800173	29.40121172543898	23.23864460891017
31	21.149869394862865	25.994957190538383	28.07339283122914	24.78178058336961
32	21.752648381947466	27.200152372659993	27.606479465970107	23.440719779422434
33	22.52630242345088	24.863227398055436	28.259867943694672	24.350602234799013
34	19.9134740966478	25.754063270933102	29.62704977506893	24.70541285735017
35	19.520388913075028	25.230735742272532	29.998548831809607	25.25032651284284
36	21.078399361485996	26.69587142649833	30.05405601509215	22.171673196923525
37	19.342258017704253	25.746807429981132	30.313815121172542	24.59711943114207
38	20.835147293571325	25.763133072123058	28.978740386010738	24.422979248294876
39	21.597373385575388	24.524198229574807	29.039689450007256	24.838738934842546
40	22.572377013495863	24.022819619793935	30.32125235814831	23.08355100856189
41	18.66184153243361	25.604048759251196	30.014693077927735	25.71941663038746
42	20.09904222899434	24.98984182266725	31.940030474532	22.97108547380641
43	21.578145407052677	24.222899434044407	30.25468001741402	23.944275141488898
44	20.912240603685966	24.333188216514294	29.276955449136555	25.47761573066318
45	20.963938470468726	23.69539979683645	28.646060078363085	26.694601654331734
46	23.013894935423014	25.14493542301553	28.141234944130023	23.69993469743143
47	19.38543027136845	24.527463358003192	32.22282687563489	23.864279494993472
48	20.29531272674503	24.21546219706864	30.520787984327384	24.96843709185895
49	21.092004063270934	22.910680597881296	32.03798432738355	23.95933101146423
50	20.192642577274704	23.129806994630677	31.32945145842403	25.348098969670584
51	20.369322304455086	22.850275721956177	28.757437236975765	28.02296473661297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	376.0
2	581.0
3	2288.5
4	3996.0
5	2685.0
6	1374.0
7	1308.0
8	1242.0
9	1232.5
10	1223.0
11	1222.0
12	1221.0
13	1234.5
14	1248.0
15	1265.0
16	1282.0
17	1231.0
18	1180.0
19	1423.5
20	1667.0
21	1755.0
22	1843.0
23	2184.5
24	2526.0
25	2979.5
26	4113.0
27	4793.0
28	5677.5
29	6562.0
30	7851.0
31	9140.0
32	10920.5
33	12701.0
34	14179.5
35	15658.0
36	17250.5
37	18843.0
38	20359.0
39	21875.0
40	24216.5
41	26558.0
42	29520.0
43	32482.0
44	36076.5
45	39671.0
46	50703.0
47	61735.0
48	59161.5
49	56588.0
50	55787.0
51	54986.0
52	48423.0
53	41860.0
54	36729.0
55	31598.0
56	28076.0
57	24554.0
58	22120.5
59	19687.0
60	17847.5
61	16008.0
62	13501.0
63	10994.0
64	9475.0
65	7956.0
66	6561.5
67	5167.0
68	4207.5
69	3248.0
70	2724.0
71	2200.0
72	1772.5
73	1345.0
74	1126.0
75	773.0
76	639.0
77	454.5
78	270.0
79	218.5
80	167.0
81	112.0
82	57.0
83	46.5
84	36.0
85	20.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.174640584022086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33548910571965	26.890458089745728
2	10.288193485626115	7.649197901740565
3	4.038874874166717	4.5043116543295545
4	2.187392484093264	3.252621176494334
5	1.4178256408878736	2.635357930540873
6	1.1333400418830508	2.5278905229889763
7	0.8845336841922948	2.3017555256016617
8	0.8018963647718353	2.384816731682148
9	0.7077956690705598	2.3680844644162953
>10	6.124159420393151	38.66510807275632
>50	0.049879890483167245	1.2935807217439552
>100	0.02469301509059532	1.801025671342138
>500	0.0029631618108714383	0.8111041198766122
>1k	0.0029631618108714383	2.9146874167408545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	4315	0.7827238426933681	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCG	3146	0.5706718908721521	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGC	3116	0.5652300101581773	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2169	0.3934479756203744	No Hit
GCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	2046	0.3711362646930779	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGC	1084	0.19663328979828762	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	971	0.17613553910898272	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	933	0.1692424902046147	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	700	0.12697721665941084	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	682	0.12371208823102597	No Hit
CGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	597	0.10829342620809752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6279204759831664E-4	0.0	0.0	0.1757727470613844	0.0
2	3.6279204759831664E-4	0.0	0.0	0.9289290378754897	0.0
3	3.6279204759831664E-4	0.0	0.0	1.2813815121172545	0.0
4	3.6279204759831664E-4	0.0	0.0	1.8567696996081846	0.0
5	3.6279204759831664E-4	0.0	0.0	3.7010230735742273	0.0
6	3.6279204759831664E-4	0.0	0.0	4.623784646640545	0.0
7	3.6279204759831664E-4	0.0	0.0	5.4723552459730085	0.0
8	3.6279204759831664E-4	0.0	0.0	6.704397039616891	0.0
9	3.6279204759831664E-4	0.0	0.0	7.152445218400812	0.0
10	3.6279204759831664E-4	0.0	0.0	8.740567406762445	0.0
11	3.6279204759831664E-4	0.0	0.0	10.057321143520534	0.0
12	3.6279204759831664E-4	0.0	0.0	11.757908866637644	0.0
13	3.6279204759831664E-4	0.0	0.0	12.272892178203454	0.0
14	5.44188071397475E-4	0.0	0.0	12.5001813960238	0.0
15	5.44188071397475E-4	0.0	0.0	12.93770860542737	0.0
16	5.44188071397475E-4	0.0	0.0	13.598352924103903	0.0
17	5.44188071397475E-4	0.0	0.0	14.475221303149034	0.0
18	5.44188071397475E-4	0.0	0.0	15.378392105645045	0.0
19	5.44188071397475E-4	0.0	0.0	16.04066898853577	0.0
20	9.069801189957916E-4	0.0	0.0	16.595378029313597	0.0
21	9.069801189957916E-4	0.0	0.0	17.244594398490786	0.0
22	9.069801189957916E-4	0.0	0.0	17.96165288056886	0.0
23	9.069801189957916E-4	0.0	0.0	18.647329850529676	0.0
24	9.069801189957916E-4	0.0	0.0	19.20022493106951	0.0
25	9.069801189957916E-4	0.0	0.0	19.69634305616021	0.0
26	9.069801189957916E-4	0.0	0.0	20.1940937454651	0.0
27	9.069801189957916E-4	0.0	0.0	20.717058482078073	0.0
28	9.069801189957916E-4	0.0	0.0	21.195218400812653	0.0
29	9.069801189957916E-4	0.0	0.0	21.697503990712523	0.0
30	9.069801189957916E-4	0.0	0.0	22.298650413582934	0.0
31	0.00108837614279495	0.0	0.0	22.8230663183863	0.0
32	0.00108837614279495	0.0	0.0	23.356914816427224	0.0
33	0.00108837614279495	0.0	0.0	23.865186475112466	0.0
34	0.00108837614279495	0.0	0.0	24.3527789870846	0.0
35	0.0012697721665941083	0.0	0.0	24.901864751124656	0.0
36	0.0012697721665941083	0.0	0.0	25.408866637643303	0.0
37	0.0012697721665941083	0.0	0.0	25.92094761282833	0.0
38	0.0012697721665941083	0.0	0.0	26.438470468727324	0.0
39	0.0012697721665941083	0.0	0.0	27.00823537948048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAGA	20	7.030088E-4	45.000004	34
CGTTATT	110	0.0	45.000004	1
CCGACGG	40	6.8048394E-9	45.000004	2
CGTAAGC	20	7.030088E-4	45.000004	43
AATAGCG	20	7.030088E-4	45.000004	1
CGATAAC	20	7.030088E-4	45.000004	10
TCGGTAT	20	7.030088E-4	45.000004	38
TACGTTC	20	7.030088E-4	45.000004	19
CGAATGC	45	3.8380676E-10	45.0	45
CGTTTTT	1160	0.0	41.702587	1
CGTAAGG	50	1.0786607E-9	40.5	2
TACGGGA	305	0.0	39.098362	4
GTTGATC	70	0.0	38.571426	16
TAATACG	140	0.0	38.571426	4
CGTTAGG	70	0.0	38.571426	2
TCAACGG	35	6.242375E-6	38.571426	2
CGATGAA	335	0.0	38.283585	19
CCCTCGA	30	1.1391514E-4	37.499996	41
TACGCGG	30	1.1391514E-4	37.499996	2
GGAACGT	30	1.1391514E-4	37.499996	8
>>END_MODULE