FastQCFastQC Report
Sat 18 Jun 2016
SRR3551003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406110
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.48509024648494253No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC14390.35433749476742754No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC10700.2634754130654256No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG10600.261013026027431No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC9570.23565043953608628No Hit
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC5730.1410947772770924No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG5570.137154958016301No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT5380.1324764226441112Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4140.10194282337297776No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT4070.10021915244638152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC207.027892E-445.0000042
TGCGACT207.027892E-445.00000434
TCCGTCG207.027892E-445.0000041
CGTAAGG453.8380676E-1045.02
CGTTATT1450.041.8965531
ACGGGCG650.041.538465
TACGGGA1200.041.2500044
CGTTTTT9950.041.155781
TAATACG556.002665E-1140.9090924
CGTTAGG556.002665E-1140.9090922
CGGGTCA501.0786607E-940.56
CGCACGG451.9230356E-840.02
ATGGGCG1250.039.6000025
CGGGTCT800.039.3750046
TACGAGG403.4508594E-739.3750042
ACATACG403.4508594E-739.37500417
TAAACGG356.2383133E-638.571432
ACGGGTA356.2383133E-638.571435
TGCGAAG356.2383133E-638.571431
TTAACGG356.2383133E-638.571432