FastQCFastQC Report
Sat 18 Jun 2016
SRR3551000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561718
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC63321.1272560252653467No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48200.8580818132942153No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC37190.6620759883073002No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG36970.6581594323130112No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC19090.3398502451408002No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT15280.2720226163306143No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG8700.15488198704688116No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT8520.15167753214246293No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT7720.13743551034504858No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC7090.12621991817958478No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT6770.12052310946061903No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC6640.11820878091853919No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT6170.10984159311255826No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT6130.10912949202268754No Hit
GAATATGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC5710.10165243057904501No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTC207.0302014E-445.00000416
ATAACCG207.0302014E-445.0000041
TACGTCA207.0302014E-445.00000417
CGTATGG351.2104465E-745.02
CGGGTCA253.8882506E-544.9999966
TACACGG502.1827873E-1144.9999962
TAATACG1950.043.846154
CCGATGA5950.042.7310918
CGATGAA6100.042.41803419
ACGGGTC750.042.05
CGAATAT750.042.014
CGTTTTT20850.041.9784131
CGTTATT2550.041.470591
GATGAAT6550.040.8778620
TATGGGT501.0786607E-940.4999964
ATCGAAT950.040.26315343
TACGGCT6600.040.2272767
CACGGGT451.9252184E-840.0000044
GCGATGA451.9252184E-840.0000049
AATACGG2150.039.767445
Produced by FastQC (version 0.11.5)