##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561718
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.008870999327065	33.0	31.0	34.0	30.0	34.0
2	32.132329745530676	33.0	31.0	34.0	30.0	34.0
3	32.063953798881286	33.0	31.0	34.0	30.0	34.0
4	35.72084925175978	37.0	35.0	37.0	33.0	37.0
5	32.048380148045815	37.0	35.0	37.0	0.0	37.0
6	33.81113298843904	37.0	35.0	37.0	17.0	37.0
7	35.441799977924866	37.0	35.0	37.0	32.0	37.0
8	35.65010200848113	37.0	35.0	37.0	33.0	37.0
9	37.582285061187285	39.0	37.0	39.0	35.0	39.0
10	37.258068995474595	39.0	37.0	39.0	34.0	39.0
11	37.21817709242004	39.0	37.0	39.0	34.0	39.0
12	37.10518267173208	39.0	37.0	39.0	34.0	39.0
13	37.02317889047529	39.0	37.0	39.0	33.0	39.0
14	38.163863362042875	40.0	37.0	41.0	33.0	41.0
15	38.26783368166945	40.0	37.0	41.0	33.0	41.0
16	38.28351948842658	40.0	37.0	41.0	34.0	41.0
17	38.24283893341499	40.0	37.0	41.0	34.0	41.0
18	38.13802477399692	40.0	37.0	41.0	34.0	41.0
19	38.00126041892907	40.0	37.0	41.0	34.0	41.0
20	37.94015680465999	40.0	36.0	41.0	33.0	41.0
21	37.94056270228121	40.0	36.0	41.0	33.0	41.0
22	37.99820728550625	40.0	36.0	41.0	34.0	41.0
23	37.992216735087716	40.0	36.0	41.0	34.0	41.0
24	37.966652305961354	40.0	36.0	41.0	34.0	41.0
25	37.73913244724221	40.0	35.0	41.0	33.0	41.0
26	37.75815800811083	40.0	35.0	41.0	33.0	41.0
27	37.72360508297758	40.0	35.0	41.0	33.0	41.0
28	37.634848803136094	40.0	35.0	41.0	33.0	41.0
29	37.59627784760325	40.0	35.0	41.0	33.0	41.0
30	37.43008057423832	40.0	35.0	41.0	33.0	41.0
31	37.36061155241598	40.0	35.0	41.0	33.0	41.0
32	37.22484947963213	40.0	35.0	41.0	32.0	41.0
33	37.07572661014958	40.0	35.0	41.0	32.0	41.0
34	36.87941280144129	40.0	35.0	41.0	31.0	41.0
35	36.79151638366582	40.0	35.0	41.0	31.0	41.0
36	36.71333124450347	40.0	35.0	41.0	31.0	41.0
37	36.65247152485767	40.0	35.0	41.0	31.0	41.0
38	36.52070789969344	39.0	35.0	41.0	30.0	41.0
39	36.41434135989945	39.0	35.0	41.0	30.0	41.0
40	36.356422617754816	39.0	35.0	41.0	30.0	41.0
41	36.21328139742718	39.0	35.0	41.0	30.0	41.0
42	36.18720247526339	39.0	35.0	41.0	30.0	41.0
43	36.12440762090586	39.0	35.0	41.0	30.0	41.0
44	36.10994306751787	39.0	35.0	41.0	30.0	41.0
45	36.08824712756223	39.0	35.0	41.0	30.0	41.0
46	35.94742023577667	39.0	35.0	41.0	29.0	41.0
47	35.81128965067881	38.0	35.0	41.0	29.0	41.0
48	35.80367194926992	38.0	35.0	41.0	29.0	41.0
49	35.78586230101225	38.0	35.0	40.0	29.0	41.0
50	35.64755802733756	38.0	35.0	40.0	29.0	41.0
51	34.65219736593807	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	12.0
10	24.0
11	21.0
12	10.0
13	13.0
14	25.0
15	25.0
16	43.0
17	83.0
18	196.0
19	356.0
20	703.0
21	1338.0
22	2082.0
23	2755.0
24	3859.0
25	4722.0
26	5966.0
27	6774.0
28	6849.0
29	7389.0
30	8795.0
31	10937.0
32	14634.0
33	21080.0
34	35648.0
35	42933.0
36	43338.0
37	65342.0
38	104292.0
39	171348.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.752495024193635	18.70707365617623	22.03596822604937	13.504463093580766
2	34.53743693454723	26.881994167892074	22.265264776987742	16.315304120572957
3	30.686038189981453	26.503512438625787	27.00447555534984	15.805973816042926
4	27.471791895577496	23.466401290327173	30.978711737918314	18.083095076177013
5	24.447498566896556	35.04249463253804	23.02578874096967	17.48421805959574
6	24.70830559106171	34.89633588384207	26.35130083066592	14.0440576944303
7	75.29703516711233	5.5668502700643385	14.465799564906234	4.670314997917104
8	75.2470100655489	8.020038524668962	11.634663656852727	5.098287752929406
9	69.26945549190164	6.917171961731687	13.310771597135929	10.502600949230754
10	40.30314143395797	24.095008527410553	20.478781167774578	15.123068870856907
11	31.809555684524977	23.029883322236426	25.74708305591062	19.413477937327983
12	27.558668228541723	20.540377912048395	30.508725018603638	21.39222884080624
13	26.269231180058323	21.543016246586365	33.064633855422116	19.1231187179332
14	20.729618776681537	26.75559622444002	31.230261447915147	21.284523550963293
15	19.590435058160857	24.034123884226606	35.872804503327295	20.502636554285246
16	23.39376697916036	24.127587152272138	30.457275714860483	22.02137015370702
17	23.133850081357547	24.827226473070116	29.77134434004251	22.267579105529823
18	23.27805055205637	24.43432469673395	30.41989040764227	21.867734343567413
19	23.36457083447566	25.642582220972088	28.25866359988464	22.734183344667606
20	25.232055942661617	26.09494443831246	29.383605296607907	19.289394322418012
21	23.407652950412842	27.496715433722972	30.33977903503181	18.755852580832375
22	22.12871939300503	22.943006989272195	31.307168365621184	23.621105252101586
23	22.828536739075478	25.339405181959627	30.717548663208227	21.11450941575666
24	23.064954300912557	24.82704844779765	29.43238422126405	22.675613030025744
25	22.63876179862493	27.730106565928097	27.593561181945386	22.03757045350158
26	21.44082974018992	24.521557080243113	29.636757234056947	24.400855945510024
27	23.514468113893447	24.671632384933364	28.379542759890192	23.43435674128299
28	20.58007754780869	25.961069433416768	29.851989788470373	23.606863230304175
29	24.68071167382922	24.139336820255004	28.284299239119985	22.895652266795793
30	22.870194652832918	25.095510558678907	28.46677514339936	23.567519645088815
31	24.058335321282208	25.83324728778497	26.593771251766903	23.514646139165915
32	27.306762467999956	25.699728333434212	26.1597456374907	20.83376356107513
33	24.470641852317353	23.833311376883064	26.820041373073323	24.87600539772626
34	22.83690392688146	24.528678091141817	29.972156847386056	22.662261134590665
35	22.628080282276873	26.153158702409396	27.52680170477001	23.691959310543727
36	23.121210287012346	27.701088446515865	27.715686518858217	21.462014747613573
37	22.090087908879543	26.371061635909832	29.049095809641134	22.489754645569484
38	21.982382619036596	27.211518947229752	26.765209589153276	24.040888844580376
39	23.635169248626536	24.20093356452882	29.048561733823735	23.11533545302091
40	22.950306025443375	23.88120017517687	29.916790987648607	23.251702811731153
41	20.370897852659166	25.595583549040622	29.664351151289438	24.369167447010778
42	21.590192943790303	25.61641250591934	30.070426797788212	22.722967752502147
43	22.761421211355167	23.936031959096912	29.27661210785483	24.02593472169309
44	22.61348220993452	23.5351190454997	28.854158136289026	24.99724060827675
45	22.267045029712417	23.42225102275519	27.547274611103795	26.7634293364286
46	23.895620222246748	25.028929106775998	27.991269640638183	23.084181030339067
47	20.39279496117269	24.943298950719043	31.832342919400837	22.83156316870743
48	20.909424301873894	25.28243709476997	29.387699877874663	24.420438725481468
49	22.10557610758423	23.237104739388805	31.62636767915573	23.03095147387123
50	21.199783521268678	23.547046738755036	30.78039158438932	24.472778155586965
51	20.857974998130736	22.94496526726934	29.139888698599652	27.05717103600027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	266.0
1	532.5
2	799.0
3	4551.5
4	8304.0
5	5274.0
6	2244.0
7	2092.0
8	1940.0
9	1995.5
10	2051.0
11	2051.5
12	2052.0
13	2003.5
14	1955.0
15	1783.0
16	1611.0
17	1646.5
18	1682.0
19	1697.0
20	1712.0
21	1833.5
22	1955.0
23	2217.0
24	2479.0
25	2816.5
26	3464.0
27	3774.0
28	4935.5
29	6097.0
30	7021.0
31	7945.0
32	8989.0
33	10033.0
34	11577.5
35	13122.0
36	14304.0
37	15486.0
38	16639.5
39	17793.0
40	19615.0
41	21437.0
42	23585.0
43	25733.0
44	29678.0
45	33623.0
46	45976.0
47	58329.0
48	53863.0
49	49397.0
50	49564.5
51	49732.0
52	45238.0
53	40744.0
54	37009.0
55	33274.0
56	31744.0
57	30214.0
58	28633.0
59	27052.0
60	25520.0
61	23988.0
62	21846.5
63	19705.0
64	16914.5
65	14124.0
66	11818.0
67	9512.0
68	8087.5
69	6663.0
70	5720.5
71	4778.0
72	3872.5
73	2967.0
74	2379.5
75	1522.0
76	1252.0
77	898.5
78	545.0
79	421.0
80	297.0
81	175.0
82	53.0
83	42.0
84	31.0
85	20.5
86	10.0
87	7.5
88	5.0
89	5.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.35517173884452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.76711501713898	26.091057916494954
2	9.611883720650685	6.98883366796119
3	3.899321061729202	4.252814605921761
4	2.2811590167505122	3.317277112703143
5	1.6525830478578571	3.0039970258787756
6	1.276521601543732	2.784489723148034
7	1.187250071225426	3.0213876165449025
8	1.0043488098193698	2.921061877334983
9	0.8688843333296854	2.8429595243452925
>10	6.371042890007332	36.624774150071204
>50	0.04912517120982317	1.2519718191983993
>100	0.021833409426537474	1.495511005927417
>500	0.005954566207237493	1.4341752107506591
>1k	0.002481069253015622	2.8273997582509534
>5k	4.962138506031244E-4	1.1422889854683238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	6332	1.1272560252653467	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4820	0.8580818132942153	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC	3719	0.6620759883073002	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG	3697	0.6581594323130112	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	1909	0.3398502451408002	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	1528	0.2720226163306143	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	870	0.15488198704688116	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	852	0.15167753214246293	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	772	0.13743551034504858	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC	709	0.12621991817958478	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	677	0.12052310946061903	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC	664	0.11820878091853919	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	617	0.10984159311255826	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT	613	0.10912949202268754	No Hit
GAATATGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC	571	0.10165243057904501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.56050544935359E-4	0.0	0.0	0.12479571599984333	0.0
2	3.56050544935359E-4	0.0	0.0	0.6759619595597791	0.0
3	3.56050544935359E-4	0.0	0.0	0.9796730743896404	0.0
4	3.56050544935359E-4	0.0	0.0	1.552558401190633	0.0
5	3.56050544935359E-4	0.0	0.0	3.4903634920013245	0.0
6	3.56050544935359E-4	0.0	0.0	4.454192317141342	0.0
7	3.56050544935359E-4	0.0	0.0	5.289842946104629	0.0
8	3.56050544935359E-4	0.0	0.0	6.379001563061892	0.0
9	3.56050544935359E-4	0.0	0.0	6.888331867591924	0.0
10	3.56050544935359E-4	0.0	0.0	8.406709416468761	0.0
11	3.56050544935359E-4	0.0	0.0	9.40418501810517	0.0
12	3.56050544935359E-4	0.0	0.0	10.920604288984864	0.0
13	3.56050544935359E-4	0.0	0.0	11.32294140476182	0.0
14	3.56050544935359E-4	0.0	0.0	11.529094670279393	0.0
15	5.340758174030386E-4	0.0	0.0	11.891910175568524	0.0
16	5.340758174030386E-4	0.0	0.0	12.397323924104265	0.0
17	5.340758174030386E-4	0.0	0.0	12.92801726133042	0.0
18	5.340758174030386E-4	0.0	0.0	13.491467248690624	0.0
19	5.340758174030386E-4	0.0	0.0	13.976942166709986	0.0
20	5.340758174030386E-4	0.0	0.0	14.385866217568246	0.0
21	5.340758174030386E-4	0.0	0.0	14.834311878914331	0.0
22	5.340758174030386E-4	0.0	0.0	15.367141519410096	0.0
23	5.340758174030386E-4	0.0	0.0	15.83249958164061	0.0
24	5.340758174030386E-4	0.0	0.0	16.217924296533138	0.0
25	5.340758174030386E-4	0.0	0.0	16.575577068920705	0.0
26	5.340758174030386E-4	0.0	0.0	16.909018404252667	0.0
27	5.340758174030386E-4	0.0	0.0	17.291060638968307	0.0
28	5.340758174030386E-4	0.0	0.0	17.633403237923655	0.0
29	5.340758174030386E-4	0.0	0.0	18.011884967189943	0.0
30	5.340758174030386E-4	0.0	0.0	18.485254166681504	0.0
31	7.12101089870718E-4	0.0	0.0	18.846289419245956	0.0
32	8.901263623383975E-4	0.0	0.0	19.235452664860304	0.0
33	8.901263623383975E-4	0.0	0.0	19.60859363595256	0.0
34	8.901263623383975E-4	0.0	0.0	20.0022075133786	0.0
35	8.901263623383975E-4	0.0	0.0	20.415404170776082	0.0
36	8.901263623383975E-4	0.0	0.0	20.80296518893822	0.0
37	0.001068151634806077	0.0	0.0	21.21277936615882	0.0
38	0.001068151634806077	0.0	0.0	21.658910698962824	0.0
39	0.001068151634806077	0.0	0.0	22.248530401375778	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTC	20	7.0302014E-4	45.000004	16
ATAACCG	20	7.0302014E-4	45.000004	1
TACGTCA	20	7.0302014E-4	45.000004	17
CGTATGG	35	1.2104465E-7	45.0	2
CGGGTCA	25	3.8882506E-5	44.999996	6
TACACGG	50	2.1827873E-11	44.999996	2
TAATACG	195	0.0	43.84615	4
CCGATGA	595	0.0	42.73109	18
CGATGAA	610	0.0	42.418034	19
ACGGGTC	75	0.0	42.0	5
CGAATAT	75	0.0	42.0	14
CGTTTTT	2085	0.0	41.978413	1
CGTTATT	255	0.0	41.47059	1
GATGAAT	655	0.0	40.87786	20
TATGGGT	50	1.0786607E-9	40.499996	4
ATCGAAT	95	0.0	40.263153	43
TACGGCT	660	0.0	40.227276	7
CACGGGT	45	1.9252184E-8	40.000004	4
GCGATGA	45	1.9252184E-8	40.000004	9
AATACGG	215	0.0	39.76744	5
>>END_MODULE