FastQCFastQC Report
Sat 18 Jun 2016
SRR3550999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503800
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42920.28541029392206413No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC32160.21385822582790265TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30960.2058784412820854No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC30060.19989360287272243TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT20840.13858225827902648TruSeq Adapter, Index 20 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCGA207.033982E-445.033
CGACGGT1050.042.85714728
TACGAAT4600.041.57608812
GCTACGA4650.040.6451610
CGTTTTT23150.040.0431981
CTACGAA4850.039.4329911
ATAGCGG2050.038.4146352
CGAATAT5000.038.25000414
GCGATGA3050.037.6229489
CGCATCG301.14008944E-437.49999621
GCGATTC1150.037.1739169
CGGTCTA1150.037.17391631
TATAGCG552.750312E-936.818181
TACGGGT1300.036.3461534
TTCACGC250.00210731536.040
GATTACG250.00210731536.01
AATAGCG900.035.01
AAGGGCG4650.034.838715
ACGGGAG6400.034.8046885
ATATGCG1100.034.7727281
Produced by FastQC (version 0.11.5)