##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1503800
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1325555260008	33.0	31.0	34.0	30.0	34.0
2	32.175215454182734	33.0	31.0	34.0	30.0	34.0
3	32.18728886820056	34.0	31.0	34.0	30.0	34.0
4	35.8375774704083	37.0	35.0	37.0	35.0	37.0
5	34.901298709934835	37.0	35.0	37.0	33.0	37.0
6	35.37689120893736	37.0	35.0	37.0	33.0	37.0
7	35.940282617369334	37.0	35.0	37.0	35.0	37.0
8	35.92539100944275	37.0	35.0	37.0	35.0	37.0
9	37.762070089107596	39.0	38.0	39.0	35.0	39.0
10	37.4126738928049	39.0	37.0	39.0	35.0	39.0
11	37.33515361085251	39.0	37.0	39.0	34.0	39.0
12	37.18589706077936	39.0	37.0	39.0	34.0	39.0
13	37.10110320521346	39.0	37.0	39.0	33.0	39.0
14	38.05490224763931	40.0	37.0	41.0	33.0	41.0
15	38.23205346455646	40.0	37.0	41.0	33.0	41.0
16	38.27930509376247	40.0	37.0	41.0	33.0	41.0
17	38.29414283814337	40.0	37.0	41.0	34.0	41.0
18	38.279297778959965	40.0	37.0	41.0	34.0	41.0
19	38.21385024604336	40.0	37.0	41.0	34.0	41.0
20	38.2075322516292	40.0	37.0	41.0	34.0	41.0
21	38.10959369597021	40.0	37.0	41.0	34.0	41.0
22	38.17783880835218	40.0	37.0	41.0	34.0	41.0
23	38.19804029791196	40.0	37.0	41.0	34.0	41.0
24	38.158610187524935	40.0	36.0	41.0	34.0	41.0
25	37.98133528394733	40.0	36.0	41.0	34.0	41.0
26	38.01717914616305	40.0	36.0	41.0	34.0	41.0
27	38.02264463359489	40.0	36.0	41.0	34.0	41.0
28	37.90220508046283	40.0	36.0	41.0	34.0	41.0
29	37.84495943609522	40.0	36.0	41.0	34.0	41.0
30	37.706949062375315	40.0	36.0	41.0	33.0	41.0
31	37.64266325309217	40.0	36.0	41.0	33.0	41.0
32	37.59869796515494	40.0	35.0	41.0	33.0	41.0
33	37.46934964755952	40.0	35.0	41.0	33.0	41.0
34	37.342475063173296	40.0	35.0	41.0	32.0	41.0
35	37.301579332358024	40.0	35.0	41.0	32.0	41.0
36	37.23293589573082	40.0	35.0	41.0	32.0	41.0
37	37.19334020481447	40.0	35.0	41.0	32.0	41.0
38	37.11807687192446	40.0	35.0	41.0	32.0	41.0
39	37.0386593961963	40.0	35.0	41.0	32.0	41.0
40	36.917010905705546	39.0	35.0	41.0	31.0	41.0
41	36.80101542758346	39.0	35.0	41.0	31.0	41.0
42	36.81390543955313	39.0	35.0	41.0	31.0	41.0
43	36.71178148689985	39.0	35.0	41.0	31.0	41.0
44	36.57879704747972	39.0	35.0	41.0	31.0	41.0
45	36.53186926452986	39.0	35.0	41.0	31.0	41.0
46	36.40360021279425	39.0	35.0	41.0	31.0	41.0
47	36.29365274637585	39.0	35.0	41.0	31.0	41.0
48	36.225309881633194	38.0	35.0	41.0	30.0	41.0
49	36.16620228753823	38.0	35.0	40.0	30.0	41.0
50	36.008310280622425	38.0	35.0	40.0	30.0	41.0
51	34.815861151748905	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	37.0
10	54.0
11	49.0
12	32.0
13	35.0
14	61.0
15	88.0
16	153.0
17	213.0
18	425.0
19	674.0
20	1141.0
21	1787.0
22	2827.0
23	4122.0
24	6013.0
25	8452.0
26	11171.0
27	13446.0
28	15321.0
29	17951.0
30	21684.0
31	28044.0
32	36927.0
33	53616.0
34	101664.0
35	111115.0
36	104267.0
37	158394.0
38	284084.0
39	519527.0
40	411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50332491022743	20.853903444606996	23.789333687990425	11.853437957175156
2	33.71891208937359	23.808352174491286	24.58990557254954	17.882830163585584
3	32.81959037105998	23.00132996409097	26.818193908764464	17.360885756084585
4	29.293190583854233	25.5241388482511	25.46030057188456	19.72236999601011
5	24.89692778294986	31.28700625083123	24.553331560047877	19.262734406171035
6	26.85091102540231	34.1945072483043	24.59702088043623	14.357560845857162
7	79.89360287272244	6.490756749567763	8.799840404309084	4.815799973400718
8	80.23560313871525	6.092831493549674	8.683601542758346	4.987963824976726
9	73.51157068759143	8.239593030988162	11.494879638249769	6.753956643170635
10	41.91441681074611	24.641774172097353	18.56583322250299	14.877975794653544
11	31.527131267455776	25.476060646362548	24.00252693177284	18.99428115440883
12	29.233874185396996	22.660925655007315	28.829498603537708	19.275701556057985
13	26.636587312142574	23.338276366538103	30.282816863944674	19.742319457374652
14	21.195371724963426	26.004588376113848	31.22502992419205	21.575009974730683
15	20.846389147493017	25.842199760606466	33.524537837478384	19.78687325442213
16	25.116238861550737	25.50698231147759	29.278361484239923	20.098417342731747
17	24.74624285144301	25.518818991887223	27.605000664982043	22.129937491687723
18	25.83967282883362	25.76492884692113	28.47113977922596	19.924258545019285
19	25.625349115573883	27.17907966484905	26.553863545684266	20.641707673892807
20	25.467016890543952	27.080795318526402	27.981447000930977	19.47074078999867
21	24.68473201223567	27.132264928846922	29.174890277962497	19.008112780954914
22	23.0524005851842	24.833820986833356	29.67395930309882	22.439819124883627
23	22.703816996941082	25.647360021279425	29.326040696901185	22.322782284878308
24	23.326772177151216	25.60533315600479	28.500664982045485	22.56722968479851
25	23.661191647825508	26.54621625216119	26.46315999468014	23.329432105333154
26	21.903910094427452	26.401117169836414	27.41967016890544	24.275302566830696
27	22.642572150551935	25.868865540630402	28.72004255885091	22.76851974996675
28	20.68420002659928	27.35456842665248	28.13638781752893	23.82484372921931
29	23.38914749301769	26.878441282085387	27.145431573347516	22.586979651549406
30	24.904375581859288	24.98596887884027	27.473866205612445	22.635789333687992
31	24.237997074078997	27.208405373054926	26.798842931240856	21.754754621625217
32	25.34758611517489	26.242984439420137	26.517688522409895	21.89174092299508
33	24.66810746109855	26.345857161856628	25.979252560180875	23.006782816863943
34	21.515627078068892	26.439220641042695	29.687524936826705	22.357627344061708
35	22.780954914217315	26.708538369464023	27.694507248304294	22.815999468014365
36	24.079797845458174	28.79172762335417	25.586979651549406	21.54149487963825
37	22.34579066365208	29.22090703551004	26.741055991488228	21.692246309349645
38	21.826905173560313	28.105333156004786	26.561909828434633	23.505851842000265
39	22.00226093895465	25.799042425854502	27.304628275036574	24.894068360154275
40	23.844793190583854	24.08638116770847	29.420202154541826	22.648623487165846
41	21.113379438755153	25.73187923926054	28.9171432371326	24.237598084851708
42	21.82890011969677	26.427184466019416	28.82138582258279	22.922529591701025
43	22.028594227955843	25.511836680409626	28.367402580130335	24.09216651150419
44	22.375182870062506	24.920135656337276	27.674690783348847	25.029990690251363
45	22.098483840936296	24.0611783481846	27.15201489559782	26.688322915281287
46	22.050804628275035	25.84532517622024	27.692778294986038	24.411091900518684
47	20.734273174624285	25.77649953451257	30.16238861550738	23.326838675355766
48	20.53092166511504	26.300904375581858	29.39393536374518	23.77423859555792
49	22.546548743183934	25.04741321984306	28.9913552334087	23.414682803564304
50	20.39593030988163	25.02054794520548	29.280755419603672	25.302766325309218
51	20.228753823646763	24.77949195371725	28.309349647559518	26.682404575076475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	570.0
1	1268.5
2	1967.0
3	4985.0
4	8003.0
5	5481.5
6	2960.0
7	2848.5
8	2737.0
9	2781.5
10	2826.0
11	2783.0
12	2740.0
13	2647.5
14	2555.0
15	2859.0
16	3163.0
17	3113.0
18	3063.0
19	3210.5
20	3358.0
21	4049.0
22	4740.0
23	6216.5
24	7693.0
25	8998.5
26	11179.5
27	12055.0
28	15707.0
29	19359.0
30	21871.0
31	24383.0
32	28350.5
33	32318.0
34	36581.0
35	40844.0
36	43516.5
37	46189.0
38	51135.0
39	56081.0
40	60547.0
41	65013.0
42	70854.5
43	76696.0
44	85785.5
45	94875.0
46	106198.5
47	117522.0
48	129146.0
49	140770.0
50	139826.5
51	138883.0
52	125222.5
53	111562.0
54	102210.5
55	92859.0
56	88375.5
57	83892.0
58	80253.0
59	76614.0
60	71482.5
61	66351.0
62	58547.5
63	50744.0
64	42944.0
65	35144.0
66	30080.5
67	25017.0
68	20054.0
69	15091.0
70	12500.5
71	9910.0
72	8354.0
73	6798.0
74	5336.5
75	2905.0
76	1935.0
77	1543.5
78	1152.0
79	871.0
80	590.0
81	462.0
82	334.0
83	262.5
84	191.0
85	109.0
86	27.0
87	25.0
88	23.0
89	18.0
90	13.0
91	8.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1503800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.919197473633332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83471886965187	18.61898263845441
2	10.660333204824395	5.52614562941148
3	4.04796125808455	3.1475972164173203
4	2.0801058389843656	2.1565869602677408
5	1.2964173558520653	1.6801048727287624
6	0.8178268008732689	1.2718448610638435
7	0.6362309424905195	1.1543416806073339
8	0.5201864808413963	1.0786252896034025
9	0.44403399215209316	1.035810425483627
>10	6.629011131024526	43.903091567398604
>50	0.9645638605456437	15.31981138896095
>100	0.06177552569965007	2.9246259105571064
>500	0.004731742367752304	0.8426253033222262
>1k	0.0021029966078899127	1.3398062557231547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4292	0.28541029392206413	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3216	0.21385822582790265	TruSeq Adapter, Index 23 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3096	0.2058784412820854	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3006	0.19989360287272243	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2084	0.13858225827902648	TruSeq Adapter, Index 20 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.319856363878176E-4	0.0	0.0	0.14882298177949196	0.0
2	5.319856363878176E-4	0.0	0.0	0.7135922330097088	0.0
3	5.319856363878176E-4	0.0	0.0	1.0434233275701557	0.0
4	5.319856363878176E-4	0.0	0.0	1.425588509110254	0.0
5	5.319856363878176E-4	0.0	0.0	2.4408165979518555	0.0
6	5.984838409362947E-4	0.0	0.0	3.176818725894401	0.0
7	5.984838409362947E-4	0.0	0.0	3.8122755685596488	0.0
8	5.984838409362947E-4	0.0	0.0	4.743582923261072	0.0
9	6.649820454847719E-4	0.0	0.0	5.161391142439154	0.0
10	6.649820454847719E-4	0.0	0.0	5.970940284612316	0.0
11	7.31480250033249E-4	0.0	0.0	7.111118499800505	0.0
12	7.31480250033249E-4	0.0	0.0	7.938821651815401	0.0
13	7.31480250033249E-4	0.0	0.0	8.29651549408166	0.0
14	7.979784545817263E-4	0.0	0.0	8.471272775635057	0.0
15	7.979784545817263E-4	0.0	0.0	8.761670434898258	0.0
16	7.979784545817263E-4	0.0	0.0	9.328235137651284	0.0
17	7.979784545817263E-4	0.0	0.0	9.935363745178881	0.0
18	7.979784545817263E-4	0.0	0.0	10.64017821518819	0.0
19	8.644766591302035E-4	0.0	0.0	11.063040297911956	0.0
20	9.309748636786807E-4	0.0	0.0	11.522609389546481	0.0
21	9.309748636786807E-4	0.0	0.0	12.056589972070753	0.0
22	9.309748636786807E-4	0.0	0.0	12.632530921665115	0.0
23	9.309748636786807E-4	0.0	0.0	13.187258944008512	0.0
24	9.309748636786807E-4	0.0	0.0	13.641242186460966	0.0
25	9.309748636786807E-4	0.0	0.0	14.053398058252426	0.0
26	9.309748636786807E-4	0.0	0.0	14.44493948663386	0.0
27	9.309748636786807E-4	0.0	0.0	14.920734140178215	0.0
28	9.309748636786807E-4	0.0	0.0	15.33455246708339	0.0
29	9.309748636786807E-4	0.0	0.0	15.786607261603937	0.0
30	9.309748636786807E-4	0.0	0.0	16.32491022742386	0.0
31	9.309748636786807E-4	0.0	0.0	16.761736933102807	0.0
32	9.309748636786807E-4	0.0	0.0	17.201356563372787	0.0
33	9.309748636786807E-4	0.0	0.0	17.645099082324776	0.0
34	9.309748636786807E-4	0.0	0.0	18.10227423859556	0.0
35	9.309748636786807E-4	0.0	0.0	18.585051203617503	0.0
36	9.309748636786807E-4	0.0	0.0	19.01915148290996	0.0
37	9.974730682271579E-4	0.0	0.0	19.488362814204017	0.0
38	9.974730682271579E-4	0.0	0.0	20.015959569091635	0.0
39	9.974730682271579E-4	0.0	0.0	20.801835350445536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGCGA	20	7.033982E-4	45.0	33
CGACGGT	105	0.0	42.857147	28
TACGAAT	460	0.0	41.576088	12
GCTACGA	465	0.0	40.64516	10
CGTTTTT	2315	0.0	40.043198	1
CTACGAA	485	0.0	39.43299	11
ATAGCGG	205	0.0	38.414635	2
CGAATAT	500	0.0	38.250004	14
GCGATGA	305	0.0	37.622948	9
CGCATCG	30	1.14008944E-4	37.499996	21
GCGATTC	115	0.0	37.173916	9
CGGTCTA	115	0.0	37.173916	31
TATAGCG	55	2.750312E-9	36.81818	1
TACGGGT	130	0.0	36.346153	4
TTCACGC	25	0.002107315	36.0	40
GATTACG	25	0.002107315	36.0	1
AATAGCG	90	0.0	35.0	1
AAGGGCG	465	0.0	34.83871	5
ACGGGAG	640	0.0	34.804688	5
ATATGCG	110	0.0	34.772728	1
>>END_MODULE