FastQCFastQC Report
Sat 18 Jun 2016
SRR3550995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444120
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC101092.276186616229848No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC100012.2518688642709175No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG86531.948347293524273No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC25550.575294965324687TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT21730.4892821759884716No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT17740.3994415923624246No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC14040.31613077546609025No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT13800.31072683058632805No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA13020.29316400972710077No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC11550.2600648473385571TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10320.23236962982977571No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT8010.1803566603620643No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT7010.15784022336305503TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATG4570.10290011708547239No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC4540.10222462397550212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG406.7993824E-945.04
GTACCGC253.8869264E-545.031
CGAACGG207.028606E-445.02
CGACGAA253.8869264E-545.019
GACGTAG207.028606E-445.01
TATGCGT207.028606E-445.011
TCTAGCG207.028606E-445.01
CGCTCGT207.028606E-445.041
CGGCTAT253.8869264E-545.031
CGGTCTA453.8380676E-1045.031
CGTTTGA207.028606E-445.034
TTCGAAT253.8869264E-545.043
TAAGACG207.028606E-445.01
TACCGAT207.028606E-445.01
TCGACAC850.042.3529434
ATCGACA850.042.3529433
CGATGAA6750.041.33333219
ACGTAGG603.6379788E-1241.2500042
AGTACGG603.6379788E-1241.2500042
TACGGCT13300.040.4323277