##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444120
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.940171575249934	33.0	31.0	34.0	30.0	34.0
2	31.966538322975772	33.0	31.0	34.0	30.0	34.0
3	32.03658921012339	33.0	31.0	34.0	30.0	34.0
4	35.71964784292533	37.0	35.0	37.0	35.0	37.0
5	34.79489777537602	37.0	35.0	37.0	33.0	37.0
6	35.22869044402414	37.0	35.0	37.0	33.0	37.0
7	35.73051652706476	37.0	35.0	37.0	33.0	37.0
8	35.616441502296674	37.0	35.0	37.0	33.0	37.0
9	37.48581914797803	39.0	37.0	39.0	35.0	39.0
10	37.22750607943799	39.0	37.0	39.0	34.0	39.0
11	37.16638070791678	39.0	37.0	39.0	34.0	39.0
12	37.08325002251644	39.0	37.0	39.0	33.0	39.0
13	36.93010447626767	39.0	37.0	39.0	33.0	39.0
14	38.05787174637485	40.0	37.0	41.0	33.0	41.0
15	38.03389849590201	40.0	37.0	41.0	33.0	41.0
16	38.20860353057732	40.0	37.0	41.0	34.0	41.0
17	38.032637575430066	40.0	37.0	41.0	33.0	41.0
18	38.02661893182023	40.0	37.0	41.0	33.0	41.0
19	37.94335990272899	40.0	37.0	41.0	33.0	41.0
20	37.748504908583264	40.0	36.0	41.0	33.0	41.0
21	37.893526524362784	40.0	36.0	41.0	33.0	41.0
22	37.88976402774025	40.0	36.0	41.0	33.0	41.0
23	37.819620823200935	40.0	36.0	41.0	33.0	41.0
24	37.796007835720076	40.0	36.0	41.0	33.0	41.0
25	37.74901378005944	40.0	36.0	41.0	33.0	41.0
26	37.656887778077994	40.0	36.0	41.0	33.0	41.0
27	37.596780149509144	40.0	36.0	41.0	33.0	41.0
28	37.45638791317662	40.0	36.0	41.0	33.0	41.0
29	37.38360353057732	40.0	36.0	41.0	32.0	41.0
30	37.333889489327206	40.0	36.0	41.0	32.0	41.0
31	37.30018463478339	40.0	35.0	41.0	32.0	41.0
32	37.0614158335585	40.0	35.0	41.0	31.0	41.0
33	36.92706926056021	39.0	35.0	41.0	31.0	41.0
34	36.721424389804554	39.0	35.0	41.0	31.0	41.0
35	36.475355759704584	39.0	35.0	41.0	30.0	41.0
36	36.512237683508964	39.0	35.0	41.0	30.0	41.0
37	36.490892101233904	39.0	35.0	41.0	30.0	41.0
38	36.373937224173645	39.0	35.0	41.0	30.0	41.0
39	36.371667567324145	39.0	35.0	41.0	30.0	41.0
40	36.099912185895704	39.0	35.0	41.0	30.0	41.0
41	36.018008646311806	39.0	35.0	41.0	29.0	41.0
42	35.94579843285599	39.0	35.0	41.0	30.0	41.0
43	35.92998739079528	39.0	35.0	41.0	29.0	41.0
44	35.96336800864631	39.0	35.0	40.0	29.0	41.0
45	35.927767270107175	38.0	35.0	40.0	29.0	41.0
46	35.79974106097451	38.0	35.0	40.0	28.0	41.0
47	35.545627307934794	38.0	35.0	40.0	28.0	41.0
48	35.5409168693146	38.0	35.0	40.0	28.0	41.0
49	35.534452400252185	38.0	35.0	40.0	28.0	41.0
50	35.40379627127803	38.0	35.0	40.0	27.0	41.0
51	34.28932720886247	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	7.0
11	11.0
12	6.0
13	11.0
14	14.0
15	14.0
16	24.0
17	70.0
18	154.0
19	305.0
20	747.0
21	1523.0
22	2085.0
23	2622.0
24	3091.0
25	3479.0
26	4038.0
27	4622.0
28	4879.0
29	5814.0
30	7348.0
31	9465.0
32	12515.0
33	17011.0
34	27248.0
35	36892.0
36	34032.0
37	49672.0
38	83223.0
39	133068.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.99918940826803	16.016166801765287	19.474241196073134	16.51040259389354
2	25.914392506529765	34.14415022966766	23.704179050707015	16.23727821309556
3	28.122804647392595	32.900117085472395	24.327434026839594	14.649644240295414
4	25.787174637485364	22.265378726470324	34.92344411420337	17.024002521840945
5	29.737233180221562	27.26627938395028	23.67085472394848	19.325632711879674
6	22.566648653517067	39.096865711969734	24.406466720706117	13.93001891380708
7	71.49666756732415	5.4863550391786005	17.95753399981987	5.059443393677385
8	70.47847428622894	12.668648113122579	10.803836800864632	6.049040799783842
9	65.61785103125281	6.342880302620914	12.097406106457715	15.941862559668557
10	40.325812843375665	23.276591912095828	19.246374853643157	17.151220390885346
11	31.339728001441053	21.469422678555343	26.203278393227052	20.98757092677655
12	25.718499504638388	18.870575520129695	31.04273619742412	24.368188777807802
13	25.285283256777447	21.691659911735567	35.49603710708817	17.527019724398812
14	20.26907142213816	29.563181122219216	28.349995496712598	21.817751958930018
15	16.804917589840585	23.306763937674503	38.69922543456724	21.18909303791768
16	18.914707736647753	23.164460055840763	29.942583085652526	27.978249121858955
17	19.95023867423219	24.534134918490498	30.502116545077907	25.013509862199406
18	20.46271278032964	23.804602359722598	29.912636224443844	25.820048635503916
19	20.983517968116725	26.620057642078716	26.452085022066107	25.94433936773845
20	24.037422318292354	24.916689183103667	30.99410069350626	20.05178780509772
21	22.46712600198145	30.269296586508148	27.37075565162569	19.892821759884715
22	19.823471133927768	22.951904890570116	30.16324416824282	27.0613798072593
23	21.21746374853643	28.341214086282985	27.887057552012966	22.554264613167614
24	22.528821039358732	24.620823200936684	27.24196163199135	25.60839412771323
25	19.482121949022787	31.900612447086374	25.344951814824825	23.27231378906602
26	18.956137980725927	24.882013870125192	29.297262001260922	26.86458614788796
27	23.289201116815274	27.046518958839954	25.375349004773483	24.288930919571285
28	18.9977933891741	26.906016391966137	30.711969737908674	23.384220480951097
29	21.62973970998829	26.022021075385034	26.87314239394758	25.475096820679095
30	21.349410069350625	30.043681887778078	27.579032693866523	21.027875349004773
31	26.9886517157525	25.14230388183374	22.35274250202648	25.516301900387283
32	26.253264883364857	27.743627848329282	23.06246059623525	22.94064667207061
33	26.224669008376118	24.258083400882644	23.841304152030983	25.67594343871026
34	27.048545438169864	24.881338377015222	24.955192290371972	23.114923894442942
35	22.24128613888138	25.639466810771864	27.163829595604795	24.955417454741962
36	25.041430244078178	28.009096640547597	27.218094208772403	19.73137890660182
37	21.860082860488156	27.150995226515356	29.044852742502027	21.94406917049446
38	26.24110600738539	23.71678825542646	25.9182203008196	24.12388543636855
39	25.885346302801043	23.331081689633432	26.16747725839863	24.61609474916689
40	25.18553544087184	22.508781410429613	31.048590471043862	21.257092677654686
41	23.05300369269567	24.721021345582276	28.43060434116905	23.795370620553
42	20.484553724218678	23.918535530937586	32.88908403134288	22.707826713500857
43	23.56390164820319	23.215347203458524	28.70868233810682	24.51206881023147
44	22.870620553003693	23.026209132666846	28.662973970998827	25.44019634333063
45	22.076690984418626	22.33337836620733	27.257948302260647	28.331982347113392
46	27.455642619111952	24.99977483563001	25.762181392416466	21.782401152841572
47	20.249482121949022	23.870575520129695	34.0702963163109	21.809646041610375
48	22.470053138791318	24.475141853553094	27.884130415203096	25.170674592452492
49	21.275330991623886	21.04116004683419	33.72714581644601	23.95636314509592
50	22.648158155453483	21.169503737728544	30.964153832297576	25.2181842745204
51	21.105106727911373	21.423489147077365	27.850580924074574	29.620823200936684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	533.5
2	920.0
3	1465.0
4	2010.0
5	1471.5
6	933.0
7	952.0
8	971.0
9	1006.0
10	1041.0
11	1026.0
12	1011.0
13	1092.0
14	1173.0
15	1072.0
16	971.0
17	1032.0
18	1093.0
19	958.0
20	823.0
21	983.5
22	1144.0
23	1286.5
24	1429.0
25	1719.0
26	2393.5
27	2778.0
28	3246.5
29	3715.0
30	4514.0
31	5313.0
32	6484.5
33	7656.0
34	8613.0
35	9570.0
36	10343.0
37	11116.0
38	12334.0
39	13552.0
40	15326.5
41	17101.0
42	18660.5
43	20220.0
44	24383.5
45	28547.0
46	49559.0
47	70571.0
48	57317.0
49	44063.0
50	42854.5
51	41646.0
52	35912.5
53	30179.0
54	27202.5
55	24226.0
56	22940.5
57	21655.0
58	19957.5
59	18260.0
60	17092.5
61	15925.0
62	14499.5
63	13074.0
64	11360.5
65	9647.0
66	8248.5
67	6850.0
68	5718.5
69	4587.0
70	3911.5
71	3236.0
72	2660.5
73	2085.0
74	1741.5
75	1059.0
76	720.0
77	547.5
78	375.0
79	297.5
80	220.0
81	155.5
82	91.0
83	65.0
84	39.0
85	27.5
86	16.0
87	12.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.38299945930471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15806022530015	26.185425435481914
2	8.601466511154772	5.914884368045243
3	3.143194096372335	3.2421732274817927
4	1.7430586566194908	2.397263393923374
5	1.1902600706876871	2.046235568344337
6	0.9373226568628823	1.9336778642466321
7	0.77910042317303	1.8751466600091655
8	0.6534000558656577	1.7972683014030864
9	0.5815875294238538	1.7997051338746857
>10	6.117629502908946	39.858532208987235
>50	0.057606785541685884	1.3385440534951603
>100	0.028803392770842942	1.9237491214432714
>500	0.0013092451259474065	0.33806846617617803
>1k	0.005236980503789626	2.8753825934758153
>5k	6.546225629737032E-4	1.9476074819057714
>10k+	0.0013092451259474065	4.526336121706351
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	10109	2.276186616229848	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	10001	2.2518688642709175	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	8653	1.948347293524273	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	2555	0.575294965324687	TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	2173	0.4892821759884716	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	1774	0.3994415923624246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	1404	0.31613077546609025	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT	1380	0.31072683058632805	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	1302	0.29316400972710077	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	1155	0.2600648473385571	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1032	0.23236962982977571	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	801	0.1803566603620643	No Hit
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	701	0.15784022336305503	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATG	457	0.10290011708547239	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	454	0.10222462397550212	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5032873998018553E-4	0.0	0.0	0.17157524993245069	0.0
2	4.5032873998018553E-4	0.0	0.0	1.2089075024768081	0.0
3	4.5032873998018553E-4	0.0	0.0	1.5430514275421057	0.0
4	4.5032873998018553E-4	0.0	0.0	2.430199045303071	0.0
5	4.5032873998018553E-4	0.0	0.0	5.666261370800685	0.0
6	4.5032873998018553E-4	0.0	0.0	6.84342069710889	0.0
7	4.5032873998018553E-4	0.0	0.0	8.022606502747005	0.0
8	4.5032873998018553E-4	0.0	0.0	9.592227325947942	0.0
9	4.5032873998018553E-4	0.0	0.0	10.176754030442224	0.0
10	4.5032873998018553E-4	0.0	0.0	13.27636674772584	0.0
11	4.5032873998018553E-4	0.0	0.0	14.4159236242457	0.0
12	4.5032873998018553E-4	0.0	0.0	17.93929568585067	0.0
13	4.5032873998018553E-4	0.0	0.0	18.549716292893812	0.0
14	4.5032873998018553E-4	0.0	0.0	18.844906781950826	0.0
15	4.5032873998018553E-4	0.0	0.0	19.455777717733945	0.0
16	4.5032873998018553E-4	0.0	0.0	20.083761145636313	0.0
17	4.5032873998018553E-4	0.0	0.0	20.80518778708457	0.0
18	4.5032873998018553E-4	0.0	0.0	21.67927587138611	0.0
19	4.5032873998018553E-4	0.0	0.0	22.514635684049356	0.0
20	6.754931099702783E-4	0.0	0.0	23.044222282266055	0.0
21	6.754931099702783E-4	0.0	0.0	23.56322615509322	0.0
22	6.754931099702783E-4	0.0	0.0	24.161037557416915	0.0
23	6.754931099702783E-4	0.0	0.0	24.691524813113574	0.0
24	6.754931099702783E-4	0.0	0.0	25.131721156444204	0.0
25	6.754931099702783E-4	0.0	0.0	25.501891380707917	0.0
26	6.754931099702783E-4	0.0	0.0	25.890975412050796	0.0
27	6.754931099702783E-4	0.0	0.0	26.344231288840852	0.0
28	6.754931099702783E-4	0.0	0.0	26.743447716833288	0.0
29	6.754931099702783E-4	0.0	0.0	27.145366117265603	0.0
30	6.754931099702783E-4	0.0	0.0	27.69791948122129	0.0
31	6.754931099702783E-4	0.0	0.0	28.10344051157345	0.0
32	6.754931099702783E-4	0.0	0.0	28.503782761415835	0.0
33	9.006574799603711E-4	0.0	0.0	28.892641628388724	0.0
34	9.006574799603711E-4	0.0	0.0	29.25042781230298	0.0
35	0.001125821849950464	0.0	0.0	29.66698189678465	0.0
36	0.001125821849950464	0.0	0.0	30.04075475096821	0.0
37	0.001125821849950464	0.0	0.0	30.41722957759164	0.0
38	0.001125821849950464	0.0	0.0	30.790101774295234	0.0
39	0.001125821849950464	0.0	0.0	31.18774205169774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	6.7993824E-9	45.0	4
GTACCGC	25	3.8869264E-5	45.0	31
CGAACGG	20	7.028606E-4	45.0	2
CGACGAA	25	3.8869264E-5	45.0	19
GACGTAG	20	7.028606E-4	45.0	1
TATGCGT	20	7.028606E-4	45.0	11
TCTAGCG	20	7.028606E-4	45.0	1
CGCTCGT	20	7.028606E-4	45.0	41
CGGCTAT	25	3.8869264E-5	45.0	31
CGGTCTA	45	3.8380676E-10	45.0	31
CGTTTGA	20	7.028606E-4	45.0	34
TTCGAAT	25	3.8869264E-5	45.0	43
TAAGACG	20	7.028606E-4	45.0	1
TACCGAT	20	7.028606E-4	45.0	1
TCGACAC	85	0.0	42.35294	34
ATCGACA	85	0.0	42.35294	33
CGATGAA	675	0.0	41.333332	19
ACGTAGG	60	3.6379788E-12	41.250004	2
AGTACGG	60	3.6379788E-12	41.250004	2
TACGGCT	1330	0.0	40.432327	7
>>END_MODULE