FastQCFastQC Report
Sat 18 Jun 2016
SRR3550994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529777
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC86591.6344612922040782No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG84161.5885929362731865No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC82171.551029961663115TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC29970.565709723147664TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC19540.3688344341109561TruSeq Adapter, Index 23 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT16730.3157932488575382Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT15360.28993331156316715No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT13940.26312958093688477No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC12040.2272654343242534No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.2210363983336385No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA11150.21046591301623138No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT10850.20480315302476323Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT7250.13685003312714594No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6050.11419899316127352No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG351.2102646E-745.00000427
CCAACGG700.045.0000042
CGAAATA253.8879483E-545.044
CGGTTGC207.029841E-445.016
TACGGGT207.029841E-445.04
CGCGAGT207.029841E-445.029
CCGTCGA207.029841E-445.036
TCCGTCG207.029841E-445.035
ATAACCG253.8879483E-545.034
TGTCGCG207.029841E-445.027
GCGTATG207.029841E-445.01
CCGTTGA207.029841E-445.041
AACTTAG302.1632786E-644.99999610
TACGAAT1300.041.5384612
CGAATAT1300.041.5384614
GCTACGA1300.041.5384610
CCGACGG501.0786607E-940.52
CGCAGTA451.9248546E-840.00000437
TACGGCT10050.039.8507467
TTCGCGG403.453515E-739.3752
Produced by FastQC (version 0.11.5)