##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529777
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00525692885875	33.0	31.0	34.0	30.0	34.0
2	32.043306900828085	33.0	31.0	34.0	30.0	34.0
3	32.09616876534844	33.0	31.0	34.0	30.0	34.0
4	35.76424986362186	37.0	35.0	37.0	35.0	37.0
5	34.859095430718966	37.0	35.0	37.0	33.0	37.0
6	35.29661914352643	37.0	35.0	37.0	33.0	37.0
7	35.79490238345568	37.0	35.0	37.0	35.0	37.0
8	35.701042136597096	37.0	35.0	37.0	35.0	37.0
9	37.526646117139855	39.0	37.0	39.0	35.0	39.0
10	37.28773616068648	39.0	37.0	39.0	34.0	39.0
11	37.247230438467504	39.0	37.0	39.0	34.0	39.0
12	37.14951385205473	39.0	37.0	39.0	34.0	39.0
13	37.03240797543117	39.0	37.0	39.0	33.0	39.0
14	38.18923433822155	40.0	37.0	41.0	33.0	41.0
15	38.19555964113202	40.0	37.0	41.0	33.0	41.0
16	38.31457386787271	40.0	37.0	41.0	34.0	41.0
17	38.200977014857195	40.0	37.0	41.0	33.0	41.0
18	38.189221125114905	40.0	37.0	41.0	34.0	41.0
19	38.117489056716316	40.0	37.0	41.0	34.0	41.0
20	37.983632736038	40.0	37.0	41.0	33.0	41.0
21	38.039594017860345	40.0	37.0	41.0	34.0	41.0
22	38.06991621002799	40.0	37.0	41.0	34.0	41.0
23	38.041338147937715	40.0	37.0	41.0	34.0	41.0
24	37.99343686116989	40.0	36.0	41.0	33.0	41.0
25	37.912163042185675	40.0	36.0	41.0	33.0	41.0
26	37.87633664730632	40.0	36.0	41.0	33.0	41.0
27	37.845061223873444	40.0	36.0	41.0	33.0	41.0
28	37.70548929077706	40.0	36.0	41.0	33.0	41.0
29	37.68489572027476	40.0	36.0	41.0	33.0	41.0
30	37.59752877153972	40.0	36.0	41.0	33.0	41.0
31	37.61701810384369	40.0	36.0	41.0	33.0	41.0
32	37.470805640863986	40.0	36.0	41.0	33.0	41.0
33	37.354418934759344	40.0	36.0	41.0	33.0	41.0
34	37.222937198104106	40.0	36.0	41.0	32.0	41.0
35	37.203412001653525	40.0	36.0	41.0	32.0	41.0
36	37.12811428204697	40.0	35.0	41.0	32.0	41.0
37	37.088103107533925	40.0	35.0	41.0	32.0	41.0
38	37.05117247445623	40.0	35.0	41.0	32.0	41.0
39	36.9280753977617	40.0	35.0	41.0	31.0	41.0
40	36.806082559265505	39.0	35.0	41.0	31.0	41.0
41	36.68417088699585	39.0	35.0	41.0	31.0	41.0
42	36.755748173288005	39.0	35.0	41.0	31.0	41.0
43	36.7011251904103	39.0	35.0	41.0	31.0	41.0
44	36.616008244978545	39.0	35.0	41.0	31.0	41.0
45	36.613962101034964	39.0	35.0	41.0	31.0	41.0
46	36.4811515033684	39.0	35.0	41.0	31.0	41.0
47	36.31813951908067	39.0	35.0	41.0	30.0	41.0
48	36.29351217587777	39.0	35.0	41.0	30.0	41.0
49	36.2979196907378	39.0	35.0	41.0	30.0	41.0
50	36.14874560428256	39.0	35.0	40.0	30.0	41.0
51	34.961296923044976	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	13.0
11	11.0
12	4.0
13	14.0
14	14.0
15	19.0
16	35.0
17	72.0
18	156.0
19	304.0
20	572.0
21	955.0
22	1820.0
23	2372.0
24	2850.0
25	3371.0
26	3963.0
27	4478.0
28	5218.0
29	6067.0
30	7458.0
31	9626.0
32	13080.0
33	18252.0
34	31672.0
35	42469.0
36	38548.0
37	56066.0
38	98179.0
39	181975.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.3259069382023	17.157030222150073	20.490885787793733	16.026177051853892
2	26.932275278088706	32.20373855414637	24.349112928647337	16.51487323911759
3	29.463151476942183	30.65988142180578	24.794205863976728	15.082761237275305
4	27.442678711986364	23.618050613748803	31.339601379448332	17.599669294816497
5	29.301762817185345	28.80325117926977	23.035352610626735	18.859633392918152
6	23.84229590941094	38.4038944688048	23.600496057775253	14.153313564009007
7	73.16153777910328	6.144472108075662	15.692829247022805	5.0011608657982505
8	72.6907736651459	11.55278541726047	9.773168710608427	5.983272206985204
9	66.05515150714358	8.280653935523814	12.092068927114616	13.572125630217997
10	39.11230574373746	26.38242128291715	18.433416324226986	16.071856649118402
11	31.865483023989338	23.878348814689954	25.555469565496423	18.70069859582428
12	27.718832640903624	20.47144364515636	29.536201080832125	22.27352263310789
13	24.788920621318024	22.74032281507125	33.77723079710897	18.693525766501754
14	20.377441829297986	29.996960985471244	27.71619001957427	21.909407165656493
15	17.503402374961542	25.34405985914828	37.2018792812825	19.950658484607676
16	20.028049537824405	24.670757696162728	29.89238868429547	25.408804081717403
17	19.846652459431045	25.597185230766904	30.213089658478946	24.3430726513231
18	20.82914131795076	25.4950667922541	29.449560852962662	24.22623103683248
19	22.491538892779413	27.023256955284957	26.73577750638476	23.749426645550862
20	24.415178461881133	25.314424748526264	30.326156099641928	19.944240689950675
21	23.305655020886146	29.883139509642735	26.978521151352364	19.83268431811876
22	20.609803747614563	24.77174358267724	28.258116150757772	26.36033651895043
23	21.373521311797226	28.71359081273819	27.445321333315714	22.467566542148866
24	22.74466426439804	24.312116230036413	27.93212993391559	25.011089571649958
25	20.130923011002743	30.25348401308475	25.848611774388093	23.766981201524416
26	19.442142637373838	25.516207762888914	30.10964990930146	24.931999690435788
27	24.126188943649876	26.357693897621076	26.29880119370981	23.217315965019246
28	19.29793101625778	26.39299176823456	31.13800712375207	23.17107009175559
29	22.963246800068706	23.100851867861383	30.57361871126908	23.36228262080083
30	23.95951504123433	26.205554412516964	28.379865490574335	21.455065055674368
31	23.69336532163533	24.318345266027027	25.986971876846294	26.001317535491346
32	25.20003699669861	25.71780201858518	28.521056973028276	20.561104011687938
33	25.0297766796218	23.36417020746465	25.91637613561933	25.68967697729422
34	22.217272550525976	22.641413273886936	32.14069315957469	23.0006210160124
35	23.488373409944185	22.021907330820326	30.50774193670167	23.981977322533822
36	22.295796155741	24.0801318290526	28.789660555290247	24.834411459916154
37	22.929836516119046	23.647308207038055	32.55407463895186	20.86878063789104
38	22.331094026354485	24.17639874890756	26.616104511898403	26.876402712839553
39	22.38300265960961	22.989861772028608	31.489664519222238	23.137471049139542
40	23.984242426530407	22.10533866136129	32.361729557908326	21.548689354199972
41	19.104075865883193	24.082963209048337	30.189117307848395	26.62384361722008
42	20.30609105340549	23.59445578045102	32.679410393429684	23.420042772713803
43	23.01647674398851	22.772600547022613	27.849264879373774	26.361657829615105
44	23.29093184490833	22.920964858799078	29.588675990086394	24.199427306206196
45	20.947115484439678	22.539861111373277	29.019568610943853	27.493454793243195
46	24.668115074833374	24.946722866413605	27.948363179224465	22.436798879528556
47	20.638872582237433	22.606115403273453	34.40787350149213	22.347138512996978
48	21.383148003782722	23.09292400387333	29.124707188118776	26.399220804225177
49	19.7800206501981	21.465635540991777	33.95881663416871	24.795527174641403
50	21.079246550907268	21.576059360825404	31.502500108536236	25.842193979731093
51	20.919367960481488	21.51358024225287	28.418183499849935	29.148868297415703
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	476.0
2	783.0
3	1502.0
4	2221.0
5	1593.5
6	966.0
7	924.0
8	882.0
9	960.0
10	1038.0
11	993.5
12	949.0
13	980.0
14	1011.0
15	981.5
16	952.0
17	984.0
18	1016.0
19	1208.5
20	1401.0
21	1748.0
22	2095.0
23	2205.0
24	2315.0
25	2825.0
26	3988.5
27	4642.0
28	5496.5
29	6351.0
30	7312.0
31	8273.0
32	9053.0
33	9833.0
34	11203.5
35	12574.0
36	14218.5
37	15863.0
38	16898.5
39	17934.0
40	19661.0
41	21388.0
42	24124.5
43	26861.0
44	31066.5
45	35272.0
46	54671.0
47	74070.0
48	63003.5
49	51937.0
50	50347.5
51	48758.0
52	42059.0
53	35360.0
54	31435.0
55	27510.0
56	25927.0
57	24344.0
58	22749.0
59	21154.0
60	20745.0
61	20336.0
62	18015.5
63	15695.0
64	13519.5
65	11344.0
66	9418.0
67	7492.0
68	6062.0
69	4632.0
70	4053.5
71	3475.0
72	2944.5
73	2414.0
74	1879.5
75	1116.5
76	888.0
77	709.5
78	531.0
79	365.5
80	200.0
81	157.5
82	115.0
83	67.5
84	20.0
85	17.0
86	14.0
87	11.5
88	9.0
89	8.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	529777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.89253094190293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34937763288372	23.711898266679697
2	9.474336637832623	6.04321148752163
3	3.403530678881089	3.256416224637931
4	1.7238713033313642	2.1991447552541628
5	1.207027021898453	1.924757332180468
6	0.8838883550808551	1.6913662028162315
7	0.7052904200450177	1.574544758101941
8	0.626613351587747	1.598742856329738
9	0.5735268010238936	1.646209912289862
>10	6.902341914773992	43.93175438895373
>50	0.09914115574873822	2.152717079657021
>100	0.0421498326835953	2.4573539997245804
>500	0.0017809788457857165	0.3481835276595657
>1k	0.00534293653735715	2.675087037684613
>5k	0.0017809788457857165	4.788612170508828
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	8659	1.6344612922040782	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	8416	1.5885929362731865	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	8217	1.551029961663115	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	2997	0.565709723147664	TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	1954	0.3688344341109561	TruSeq Adapter, Index 23 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	1673	0.3157932488575382	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	1536	0.28993331156316715	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	1394	0.26312958093688477	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	1204	0.2272654343242534	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1171	0.2210363983336385	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	1115	0.21046591301623138	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	1085	0.20480315302476323	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	725	0.13685003312714594	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	605	0.11419899316127352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.662759991468108E-4	0.0	0.0	0.21442984501025902	0.0
2	5.662759991468108E-4	0.0	0.0	1.3581186046204345	0.0
3	5.662759991468108E-4	0.0	0.0	1.7499815960300278	0.0
4	5.662759991468108E-4	0.0	0.0	2.4919919135787323	0.0
5	5.662759991468108E-4	0.0	0.0	5.163115801554238	0.0
6	5.662759991468108E-4	0.0	0.0	6.360978298416126	0.0
7	5.662759991468108E-4	0.0	0.0	7.48541367405531	0.0
8	5.662759991468108E-4	0.0	0.0	8.989631486455622	0.0
9	7.550346655290811E-4	0.0	0.0	9.561192728261137	0.0
10	7.550346655290811E-4	0.0	0.0	12.195886193624865	0.0
11	7.550346655290811E-4	0.0	0.0	13.313337498607904	0.0
12	7.550346655290811E-4	0.0	0.0	16.087146101095367	0.0
13	7.550346655290811E-4	0.0	0.0	16.612650228303607	0.0
14	7.550346655290811E-4	0.0	0.0	16.870305807915408	0.0
15	7.550346655290811E-4	0.0	0.0	17.398830073785764	0.0
16	7.550346655290811E-4	0.0	0.0	18.06779078744453	0.0
17	7.550346655290811E-4	0.0	0.0	18.803760827669	0.0
18	7.550346655290811E-4	0.0	0.0	19.71074621963581	0.0
19	7.550346655290811E-4	0.0	0.0	20.48899820112991	0.0
20	7.550346655290811E-4	0.0	0.0	21.0288479869832	0.0
21	7.550346655290811E-4	0.0	0.0	21.560392391515677	0.0
22	7.550346655290811E-4	0.0	0.0	22.2042482025456	0.0
23	7.550346655290811E-4	0.0	0.0	22.765805235032854	0.0
24	9.437933319113513E-4	0.0	0.0	23.19957265037931	0.0
25	9.437933319113513E-4	0.0	0.0	23.618994407080717	0.0
26	9.437933319113513E-4	0.0	0.0	24.022937953138772	0.0
27	9.437933319113513E-4	0.0	0.0	24.456894127151614	0.0
28	9.437933319113513E-4	0.0	0.0	24.853853602553528	0.0
29	9.437933319113513E-4	0.0	0.0	25.25836342461073	0.0
30	9.437933319113513E-4	0.0	0.0	25.807839902449523	0.0
31	9.437933319113513E-4	0.0	0.0	26.25331035511168	0.0
32	9.437933319113513E-4	0.0	0.0	26.660462798498237	0.0
33	9.437933319113513E-4	0.0	0.0	27.06553889655459	0.0
34	9.437933319113513E-4	0.0	0.0	27.469293683946265	0.0
35	9.437933319113513E-4	0.0	0.0	27.89041426864511	0.0
36	9.437933319113513E-4	0.0	0.0	28.286429950715114	0.0
37	9.437933319113513E-4	0.0	0.0	28.700000188758665	0.0
38	9.437933319113513E-4	0.0	0.0	29.109984012140956	0.0
39	9.437933319113513E-4	0.0	0.0	29.48769010357188	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATCG	35	1.2102646E-7	45.000004	27
CCAACGG	70	0.0	45.000004	2
CGAAATA	25	3.8879483E-5	45.0	44
CGGTTGC	20	7.029841E-4	45.0	16
TACGGGT	20	7.029841E-4	45.0	4
CGCGAGT	20	7.029841E-4	45.0	29
CCGTCGA	20	7.029841E-4	45.0	36
TCCGTCG	20	7.029841E-4	45.0	35
ATAACCG	25	3.8879483E-5	45.0	34
TGTCGCG	20	7.029841E-4	45.0	27
GCGTATG	20	7.029841E-4	45.0	1
CCGTTGA	20	7.029841E-4	45.0	41
AACTTAG	30	2.1632786E-6	44.999996	10
TACGAAT	130	0.0	41.53846	12
CGAATAT	130	0.0	41.53846	14
GCTACGA	130	0.0	41.53846	10
CCGACGG	50	1.0786607E-9	40.5	2
CGCAGTA	45	1.9248546E-8	40.000004	37
TACGGCT	1005	0.0	39.850746	7
TTCGCGG	40	3.453515E-7	39.375	2
>>END_MODULE