SRR3550989_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686566
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	12647	1.8420661669817613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	11823	1.7220485721693182	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	10534	1.534302601643542	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	4117	0.5996510167995502	Illumina PCR Primer Index 10 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	2747	0.40010720018177426	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT	2254	0.3283005566835526	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1849	0.26931132622355314	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC	1780	0.2592613091822199	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1669	0.24309389046355342	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTA	1603	0.23348083068488681	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1594	0.23216995889688685	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT	1555	0.2264895144822202	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	1152	0.1677915888639985	Illumina PCR Primer Index 10 (95% over 24bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	712	0.10370452367288796	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTCGG	30	2.164219E-6	45.000004	2
TATAGCG	30	2.164219E-6	45.000004	1
TCGCAAT	20	7.0313E-4	45.0	42
GCGCGAT	20	7.0313E-4	45.0	22
CGACAAT	20	7.0313E-4	45.0	21
TAGCCGT	20	7.0313E-4	45.0	44
CGTAGTG	20	7.0313E-4	45.0	19
TGCGAAC	20	7.0313E-4	45.0	13
GATGCGA	20	7.0313E-4	45.0	11
CTTTACG	20	7.0313E-4	45.0	1
GGCCGAT	100	0.0	40.5	8
CGTATGG	95	0.0	40.263157	2
GTACGAG	45	1.9263098E-8	40.0	1
TAAGCAT	85	0.0	39.705883	24
CGATGAA	910	0.0	39.56044	19
CGAACAC	40	3.455516E-7	39.375	12
TACGGCT	1480	0.0	39.375	7
CTACGAA	80	0.0	39.375	11
CCAACGT	40	3.455516E-7	39.375	12
CCGATTA	75	0.0	39.000004	18