##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550989_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686566
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930139272844855	33.0	31.0	34.0	30.0	34.0
2	31.96577459413953	33.0	31.0	34.0	30.0	34.0
3	32.02406177993085	33.0	31.0	34.0	30.0	34.0
4	35.70382745431612	37.0	35.0	37.0	33.0	37.0
5	34.74484317603843	37.0	35.0	37.0	33.0	37.0
6	35.193972611518774	37.0	35.0	37.0	33.0	37.0
7	35.71801982620753	37.0	35.0	37.0	33.0	37.0
8	35.62198390249444	37.0	35.0	37.0	33.0	37.0
9	37.44831232539916	39.0	37.0	39.0	35.0	39.0
10	37.22013032978621	39.0	37.0	39.0	34.0	39.0
11	37.16654917371382	39.0	37.0	39.0	34.0	39.0
12	37.097929405184644	39.0	37.0	39.0	34.0	39.0
13	36.946761127116694	39.0	37.0	39.0	33.0	39.0
14	38.072444601101715	40.0	37.0	41.0	33.0	41.0
15	38.0974137956147	40.0	37.0	41.0	33.0	41.0
16	38.21938021981863	40.0	37.0	41.0	34.0	41.0
17	38.06273104115264	40.0	37.0	41.0	33.0	41.0
18	38.076224281423784	40.0	37.0	41.0	33.0	41.0
19	38.000517066094154	40.0	37.0	41.0	33.0	41.0
20	37.83946481474469	40.0	36.0	41.0	33.0	41.0
21	37.900765549124195	40.0	36.0	41.0	33.0	41.0
22	37.926259092352375	40.0	36.0	41.0	33.0	41.0
23	37.86515061916844	40.0	36.0	41.0	33.0	41.0
24	37.83552928633227	40.0	36.0	41.0	33.0	41.0
25	37.755936064413326	40.0	36.0	41.0	33.0	41.0
26	37.69015506156728	40.0	36.0	41.0	33.0	41.0
27	37.62693317175625	40.0	36.0	41.0	33.0	41.0
28	37.467261705356805	40.0	36.0	41.0	32.0	41.0
29	37.42561530865205	40.0	36.0	41.0	32.0	41.0
30	37.35977895788606	40.0	36.0	41.0	32.0	41.0
31	37.318616127218654	40.0	36.0	41.0	32.0	41.0
32	37.19847909742108	40.0	35.0	41.0	32.0	41.0
33	37.07663356472648	40.0	35.0	41.0	32.0	41.0
34	36.943210121095426	40.0	35.0	41.0	31.0	41.0
35	36.89819769694392	40.0	35.0	41.0	31.0	41.0
36	36.79019788337902	39.0	35.0	41.0	31.0	41.0
37	36.78561565821786	39.0	35.0	41.0	31.0	41.0
38	36.62234075092562	39.0	35.0	41.0	30.0	41.0
39	36.504251594165744	39.0	35.0	41.0	30.0	41.0
40	36.358210281313085	39.0	35.0	41.0	30.0	41.0
41	36.20094499290673	39.0	35.0	41.0	30.0	41.0
42	36.26393820841696	39.0	35.0	41.0	30.0	41.0
43	36.21033374795722	39.0	35.0	41.0	30.0	41.0
44	36.14370359149739	39.0	35.0	41.0	30.0	41.0
45	36.114915390508706	39.0	35.0	41.0	30.0	41.0
46	35.925023959823235	38.0	35.0	40.0	29.0	41.0
47	35.713360405263295	38.0	35.0	40.0	29.0	41.0
48	35.69057454053944	38.0	35.0	40.0	28.0	41.0
49	35.67585490688441	38.0	35.0	40.0	28.0	41.0
50	35.5215638409126	38.0	35.0	40.0	28.0	41.0
51	34.304362581310464	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	8.0
10	11.0
11	8.0
12	12.0
13	12.0
14	20.0
15	25.0
16	41.0
17	107.0
18	172.0
19	486.0
20	1063.0
21	1890.0
22	2966.0
23	3685.0
24	4357.0
25	5088.0
26	6140.0
27	6846.0
28	7539.0
29	8852.0
30	11034.0
31	14470.0
32	19005.0
33	25595.0
34	41803.0
35	56298.0
36	52082.0
37	75475.0
38	126341.0
39	214983.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.95372913893202	16.669919570733185	19.14936073152472	16.22699055881008
2	26.584334208218873	32.80267301322815	23.682792331691346	16.930200446861626
3	29.03624706146241	31.79053434047127	24.15776487621001	15.015453721856312
4	26.869812953161095	23.016141201282906	32.226326383770825	17.887719461785174
5	29.362071526990853	28.815437991394855	22.649534057905576	19.17295642370872
6	23.943218860240677	38.8562789302121	23.181748003833572	14.018754205713654
7	72.25918556992336	6.59251987427283	16.239079709743855	4.909214846059956
8	71.2559316948407	11.988650763364337	10.474302543382572	6.281114998412389
9	64.92354704427542	8.427449072631036	12.376523160191445	14.272480722902095
10	38.95546822883743	26.241614061867324	18.660551207021612	16.142366502273635
11	32.330176559864604	24.658226594384224	24.228697605182894	18.782899240568277
12	27.142474285065095	20.478439072135817	29.153205955436185	23.225880687362903
13	25.231660175423777	22.594768747651354	33.55555037680281	18.618020700122056
14	19.754109583055378	31.06329180297306	26.92312173920643	22.259476874765134
15	17.860045501816284	24.539082914097115	36.89972996041167	20.701141623674925
16	19.172082509183387	25.355901690441996	29.66619378180685	25.80582201856777
17	19.007203968737162	25.840195989897545	29.802378795337958	25.35022124602733
18	20.73231124174515	24.872626957932667	29.22617781830152	25.168883982020667
19	22.665410171782465	26.327840295033546	26.158737834381547	24.84801169880245
20	25.702117494894882	24.64307874261178	29.40431072904863	20.25049303344471
21	23.469411535089122	30.118298896245953	26.445818755953542	19.96647081271138
22	20.31035617843004	24.417171837813118	28.463541742527305	26.80893024122954
23	21.570540923960696	28.587783257545524	27.336192004847316	22.505483813646467
24	22.63074489561091	24.21617149698645	27.13082209139398	26.022261516008662
25	20.087507974470043	31.270700850318832	25.184614443476665	23.457176731734457
26	19.661911600632713	25.23355365689533	28.209669572917974	26.894865169553984
27	24.254915040942894	26.976721830093535	24.464217569760226	24.304145559203338
28	19.19407602473761	28.299391464185526	29.16194510068952	23.344587410387348
29	21.268457803037144	23.53582903901446	27.46902701269798	27.72668614525042
30	19.887964157852267	27.473542237745534	27.36634205597131	25.272151548430887
31	21.93569154312914	27.957690884780195	23.470285449614458	26.636332122476208
32	24.77168983025667	27.32089850065398	25.80086983625755	22.1065418328318
33	21.45358202998692	25.356192995283777	25.908361322873546	27.28186365185576
34	21.82164569757314	27.25914187419709	25.880978667746433	25.03823376048333
35	23.26957641362957	23.30759169548157	25.18592531526466	28.236906575624193
36	20.375171505725596	28.14354337383442	25.01216197714422	26.469123143295764
37	19.866116294718935	26.189907452451767	29.81257446480018	24.13140178802912
38	20.927631138157146	26.91452824637398	26.77091495937754	25.38692565609133
39	22.446640235607358	25.253362386136217	28.156797744135304	24.143199634121117
40	23.806596889446897	23.105134830446016	30.972841649601058	22.115426630506025
41	19.467611271166938	27.402172551509977	25.369010408321994	27.761205769001084
42	23.62453136333579	23.971912387155786	28.457715645691746	23.945840603816677
43	23.28647209445268	23.92311882615801	25.56899118220244	27.221417897186868
44	22.138876670269138	24.477326287640228	28.040275807424198	25.343521234666444
45	20.522717408086038	23.844029561615343	26.761738856861538	28.871514173437074
46	24.457954515662006	26.12043124768777	26.16878785142288	23.252826385227348
47	20.111103666654042	24.18529318375801	32.130195785984164	23.573407363603792
48	21.99803077926958	24.61977435526956	27.058578490633096	26.32361637482777
49	20.84475491067137	22.104794003781137	32.03217170672594	25.018279378821557
50	20.915687639644258	22.935595412531352	29.715132995225513	26.433583952598877
51	20.342836668288264	23.224132858312238	26.363233833309547	30.069796640089958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	638.0
2	1044.0
3	2070.0
4	3096.0
5	2248.5
6	1401.0
7	1332.0
8	1263.0
9	1253.5
10	1244.0
11	1268.0
12	1292.0
13	1328.0
14	1364.0
15	1527.0
16	1690.0
17	1674.5
18	1659.0
19	1719.0
20	1779.0
21	2388.0
22	2997.0
23	3009.5
24	3022.0
25	3582.0
26	4576.5
27	5011.0
28	6229.0
29	7447.0
30	9283.0
31	11119.0
32	11667.0
33	12215.0
34	13224.0
35	14233.0
36	15680.0
37	17127.0
38	18540.5
39	19954.0
40	22872.5
41	25791.0
42	30420.5
43	35050.0
44	39629.0
45	44208.0
46	71950.5
47	99693.0
48	82543.0
49	65393.0
50	63586.0
51	61779.0
52	52291.0
53	42803.0
54	39343.5
55	35884.0
56	33862.5
57	31841.0
58	30336.5
59	28832.0
60	28546.0
61	28260.0
62	25740.5
63	23221.0
64	19768.0
65	16315.0
66	13722.0
67	11129.0
68	9437.5
69	7746.0
70	6786.5
71	5827.0
72	4937.0
73	4047.0
74	3353.0
75	1940.5
76	1222.0
77	990.5
78	759.0
79	578.0
80	397.0
81	342.0
82	287.0
83	172.0
84	57.0
85	33.5
86	10.0
87	10.5
88	11.0
89	8.0
90	5.0
91	5.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.31394662561034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.13061777244553	23.839501015011013
2	9.809076813053261	6.143218155409236
3	3.3249109428882964	3.1234825140153557
4	1.6652797442196159	2.085859241088124
5	0.9843501369332819	1.541194382442051
6	0.6794860308640117	1.276643358199409
7	0.5422007045425635	1.1884910745689907
8	0.45294469075054	1.1346788696412793
9	0.3854660945867146	1.0863418240683762
>10	5.638821141033053	40.8338392517639
>50	0.3218258753744022	6.128463781576303
>100	0.057067960458057815	3.1710870815696692
>500	0.001871080670755994	0.34348927000234997
>1k	0.004677701676889985	2.976418749018231
>5k	0.0	0.0
>10k+	0.0014033105030669952	5.127291431625697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	12647	1.8420661669817613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	11823	1.7220485721693182	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	10534	1.534302601643542	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	4117	0.5996510167995502	Illumina PCR Primer Index 10 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	2747	0.40010720018177426	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT	2254	0.3283005566835526	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1849	0.26931132622355314	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC	1780	0.2592613091822199	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1669	0.24309389046355342	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTA	1603	0.23348083068488681	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1594	0.23216995889688685	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT	1555	0.2264895144822202	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	1152	0.1677915888639985	Illumina PCR Primer Index 10 (95% over 24bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	712	0.10370452367288796	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.282621044444379E-4	0.0	0.0	0.17827856316799842	0.0
2	7.282621044444379E-4	0.0	0.0	1.269797805309322	0.0
3	7.282621044444379E-4	0.0	0.0	1.6396093019462077	0.0
4	7.282621044444379E-4	0.0	0.0	2.4494367620884225	0.0
5	7.282621044444379E-4	0.0	0.0	5.38928522530973	0.0
6	7.282621044444379E-4	0.0	0.0	6.6453917030554965	0.0
7	7.282621044444379E-4	0.0	0.0	7.771284916526597	0.0
8	7.282621044444379E-4	0.0	0.0	9.339087574974585	0.0
9	7.282621044444379E-4	0.0	0.0	9.92359074000169	0.0
10	7.282621044444379E-4	0.0	0.0	12.565288697663444	0.0
11	7.282621044444379E-4	0.0	0.0	13.635105729092324	0.0
12	7.282621044444379E-4	0.0	0.0	16.51742148606252	0.0
13	7.282621044444379E-4	0.0	0.0	17.066968070076292	0.0
14	7.282621044444379E-4	0.0	0.0	17.34370766976518	0.0
15	7.282621044444379E-4	0.0	0.0	17.90767384344695	0.0
16	7.282621044444379E-4	0.0	0.0	18.58481194815939	0.0
17	7.282621044444379E-4	0.0	0.0	19.279428343378495	0.0
18	7.282621044444379E-4	0.0	0.0	20.185240748886486	0.0
19	7.282621044444379E-4	0.0	0.0	20.904181098394037	0.0
20	7.282621044444379E-4	0.0	0.0	21.433045038641588	0.0
21	7.282621044444379E-4	0.0	0.0	21.94719808437936	0.0
22	7.282621044444379E-4	0.0	0.0	22.531555596985577	0.0
23	7.282621044444379E-4	0.0	0.0	23.04177602735935	0.0
24	7.282621044444379E-4	0.0	0.0	23.46343978583268	0.0
25	7.282621044444379E-4	0.0	0.0	23.83135780099801	0.0
26	7.282621044444379E-4	0.0	0.0	24.205538870261563	0.0
27	7.282621044444379E-4	0.0	0.0	24.616278697168227	0.0
28	7.282621044444379E-4	0.0	0.0	24.982011926020224	0.0
29	8.739145253333256E-4	0.0	0.0	25.364349530853552	0.0
30	8.739145253333256E-4	0.0	0.0	25.885493892793992	0.0
31	0.0010195669462222132	0.0	0.0	26.295068500333546	0.0
32	0.0010195669462222132	0.0	0.0	26.684980031053097	0.0
33	0.0010195669462222132	0.0	0.0	27.053480655901982	0.0
34	0.0010195669462222132	0.0	0.0	27.437566089785978	0.0
35	0.0010195669462222132	0.0	0.0	27.835051546391753	0.0
36	0.0010195669462222132	0.0	0.0	28.21272827375664	0.0
37	0.0010195669462222132	0.0	0.0	28.587637605124634	0.0
38	0.0010195669462222132	0.0	0.0	28.9538077912393	0.0
39	0.0010195669462222132	0.0	0.0	29.323327983034407	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTCGG	30	2.164219E-6	45.000004	2
TATAGCG	30	2.164219E-6	45.000004	1
TCGCAAT	20	7.0313E-4	45.0	42
GCGCGAT	20	7.0313E-4	45.0	22
CGACAAT	20	7.0313E-4	45.0	21
TAGCCGT	20	7.0313E-4	45.0	44
CGTAGTG	20	7.0313E-4	45.0	19
TGCGAAC	20	7.0313E-4	45.0	13
GATGCGA	20	7.0313E-4	45.0	11
CTTTACG	20	7.0313E-4	45.0	1
GGCCGAT	100	0.0	40.5	8
CGTATGG	95	0.0	40.263157	2
GTACGAG	45	1.9263098E-8	40.0	1
TAAGCAT	85	0.0	39.705883	24
CGATGAA	910	0.0	39.56044	19
CGAACAC	40	3.455516E-7	39.375	12
TACGGCT	1480	0.0	39.375	7
CTACGAA	80	0.0	39.375	11
CCAACGT	40	3.455516E-7	39.375	12
CCGATTA	75	0.0	39.000004	18
>>END_MODULE