FastQCFastQC Report
Sat 18 Jun 2016
SRR3550988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920796
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC73850.8020234666527657No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC59150.642378985138945No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG50720.5508277620667336No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC32460.3525210795876611TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.26151286495597287No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC23020.25000108601688104TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT19330.20992706310626894No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT13420.14574346543642674TruSeq Adapter, Index 20 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT11820.12836719533968435No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC9430.10241139188267541No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.0325565E-445.01
CGACGGT351.2115743E-745.028
CGGGTCA650.041.5384646
CTAGACG451.927583E-840.0000041
TCGATAG403.4572258E-739.3751
CACGGGC1750.038.5714264
ACCGCTT356.246937E-638.57142645
ACGGGCT2850.037.8947375
ACGATTC301.1397454E-437.50000411
GCCGATA301.1397454E-437.5000049
GTCGTAG301.1397454E-437.5000041
TTCGAAC301.1397454E-437.50000425
GCGTATG601.546141E-1037.5000041
ATGGGCG3500.037.2857135
AAGGGCG2250.037.05
CGTTTTT11900.036.1134451
CCCGTAC250.00210689136.00000433
AACGACT250.00210689136.00000433
TAGTGCG504.8770744E-836.0000041
CCAAACG250.00210689136.0000041
Produced by FastQC (version 0.11.5)