FastQCFastQC Report
Sat 18 Jun 2016
SRR3550985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585997
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC77571.323726913277713No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC70241.1986409486737988No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG58140.9921552499415527No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC22230.37935347791882895No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT19090.3257695858511221No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT13520.2307179046991708No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC10600.18088829806295936No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.16587115633697783No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA9030.15409635202910596No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT6800.11604154970076638No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC6120.10443739473068976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.2105556E-745.00000425
TTTCGAC302.1636733E-645.00000424
ATTACGG302.1636733E-645.0000042
CGGTCTA351.2105556E-745.00000431
TCGACGT302.1636733E-645.00000426
TTCGACG302.1636733E-645.00000425
TTTAGCG253.8884573E-545.01
TTTACGG406.8048394E-945.02
GCGAATG253.8884573E-545.01
TAGCGTA207.030453E-445.025
TACGGGT950.042.631584
TACGGCT8200.042.5304877
GCGTTAG650.041.538461
CGGCGAA603.6379788E-1241.25000431
TTGTACG556.002665E-1140.9090921
GCCGATT556.002665E-1140.9090929
ACGGGTC1050.040.7142835
TTACGGG1500.040.53
CGTTAGG800.039.3752
ACGGTCT403.4543518E-739.37530