##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	585997
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0267185668186	33.0	31.0	34.0	30.0	34.0
2	32.10553466997271	33.0	31.0	34.0	30.0	34.0
3	32.00574746969694	33.0	31.0	34.0	30.0	34.0
4	35.72109242880083	37.0	35.0	37.0	33.0	37.0
5	33.46138290810362	37.0	35.0	37.0	30.0	37.0
6	34.49818173130579	37.0	35.0	37.0	30.0	37.0
7	35.62361752705219	37.0	35.0	37.0	32.0	37.0
8	35.47867821848917	37.0	35.0	37.0	33.0	37.0
9	37.42154140720857	39.0	37.0	39.0	34.0	39.0
10	37.124852175011135	39.0	37.0	39.0	34.0	39.0
11	37.023855070930395	39.0	37.0	39.0	33.0	39.0
12	36.98860915670217	39.0	37.0	39.0	33.0	39.0
13	36.991912927881884	39.0	37.0	39.0	33.0	39.0
14	38.169065711940505	40.0	37.0	41.0	33.0	41.0
15	38.20131673029043	40.0	37.0	41.0	33.0	41.0
16	38.1169135678169	40.0	37.0	41.0	34.0	41.0
17	37.99010575139463	40.0	37.0	41.0	33.0	41.0
18	37.862582914247	40.0	36.0	41.0	33.0	41.0
19	37.74922397213638	39.0	36.0	41.0	33.0	41.0
20	37.92841430928827	40.0	36.0	41.0	33.0	41.0
21	37.96233939764197	40.0	36.0	41.0	34.0	41.0
22	38.097256470596264	40.0	37.0	41.0	34.0	41.0
23	38.173241501236355	40.0	37.0	41.0	34.0	41.0
24	38.03259743650565	40.0	36.0	41.0	34.0	41.0
25	37.762227451676374	40.0	36.0	41.0	33.0	41.0
26	37.79952969042504	40.0	36.0	41.0	33.0	41.0
27	37.883318515282504	40.0	36.0	41.0	34.0	41.0
28	37.75715063387697	40.0	36.0	41.0	33.0	41.0
29	37.78372414875844	40.0	36.0	41.0	33.0	41.0
30	37.590898929516705	40.0	36.0	41.0	33.0	41.0
31	37.532325250811866	40.0	36.0	41.0	33.0	41.0
32	37.48596153222628	40.0	36.0	41.0	33.0	41.0
33	37.448592740235874	40.0	36.0	41.0	33.0	41.0
34	37.298723372303954	40.0	36.0	41.0	33.0	41.0
35	37.24911902279363	40.0	35.0	41.0	32.0	41.0
36	37.24973677339645	40.0	35.0	41.0	33.0	41.0
37	37.26040406350203	40.0	35.0	41.0	33.0	41.0
38	37.12263544011317	40.0	35.0	41.0	32.0	41.0
39	36.997962446906726	40.0	35.0	41.0	32.0	41.0
40	36.88058300639764	39.0	35.0	41.0	31.0	41.0
41	36.72581088299087	39.0	35.0	41.0	31.0	41.0
42	36.788988680829426	39.0	35.0	41.0	31.0	41.0
43	36.786420408295605	39.0	35.0	41.0	32.0	41.0
44	36.77804664528999	39.0	35.0	41.0	31.0	41.0
45	36.728423524352515	39.0	35.0	41.0	31.0	41.0
46	36.60641436730905	39.0	35.0	41.0	31.0	41.0
47	36.48120553518192	39.0	35.0	41.0	31.0	41.0
48	36.438679720203346	39.0	35.0	41.0	31.0	41.0
49	36.37678691187839	39.0	35.0	41.0	31.0	41.0
50	36.30263294863284	39.0	35.0	41.0	31.0	41.0
51	35.03738756341756	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	26.0
10	27.0
11	26.0
12	17.0
13	15.0
14	18.0
15	22.0
16	52.0
17	80.0
18	173.0
19	347.0
20	667.0
21	1177.0
22	1736.0
23	2189.0
24	2762.0
25	3423.0
26	4201.0
27	4820.0
28	5359.0
29	6411.0
30	8085.0
31	10569.0
32	14586.0
33	21931.0
34	39639.0
35	46554.0
36	43888.0
37	67932.0
38	116061.0
39	183129.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.954330824219234	18.501118606409246	21.622806942697657	13.921743626673857
2	29.009022230489233	29.25356273154982	24.995520454882875	16.74189458307807
3	30.132918769208715	28.94724717020736	25.2315284890537	15.688305571530229
4	27.38648832673205	24.216676877185378	30.209881620554373	18.186953175528203
5	27.406624948591883	31.922518374667447	22.170250018344802	18.500606658395863
6	25.334259390406434	36.700870482272094	23.611724974701236	14.353145152620236
7	77.71746271738593	5.773749694964309	12.408766597781218	4.100020989868549
8	78.48845642554484	8.654651815623629	8.295946907578026	4.560944851253504
9	72.7592462077451	6.435698476272063	9.90312237093364	10.901932945049207
10	43.48827724374015	22.586975701240792	17.77858931018418	16.14615774483487
11	33.34607515055538	24.30882751959481	23.95063455956259	18.39446277028722
12	28.316527217716132	22.935953597032068	27.318740539627335	21.428778645624465
13	25.980679081974824	23.84909820357442	30.751010670703096	19.419212043747663
14	21.96325237159917	28.724037836371174	27.827958163608347	21.484751628421307
15	20.91222310011826	24.504391660708162	34.676798686682694	19.906586552490882
16	24.00131741288778	25.9277777872583	28.03888074512327	22.032024054730655
17	24.531524905417605	25.563953399078837	28.449292402520832	21.45522929298273
18	24.45097841797825	24.85575864722857	29.09383495137347	21.599427983419712
19	24.921458642279738	27.10713536076123	25.62555781002292	22.34584818693611
20	27.37061793831709	26.767884477224285	27.679834538402076	18.18166304605655
21	25.446546654675707	29.62353049588991	26.61361747585739	18.316305373576995
22	24.077768316220048	24.78545112005693	28.306970854799594	22.829809708923424
23	23.903194043655514	27.87949426362251	28.291953713073614	19.925357979648357
24	24.496200492493987	24.919752148901786	27.55867350856745	23.025373850036775
25	22.666327643315583	29.350832854093113	25.894501166388224	22.088338336203087
26	21.49072435524414	25.97317051111183	29.251514939496275	23.284590194147754
27	23.00472528016355	27.124029645202963	27.09689640049352	22.77434867413997
28	19.78610812000744	27.04075276835888	30.50988315639841	22.663255955235265
29	22.424688180997514	25.101323044315926	29.687012049549743	22.786976725136817
30	21.786630306981095	26.55389020762905	30.925414293929833	20.734065191460026
31	24.96019604195926	24.567361266354606	28.43103292337674	22.041409768309393
32	25.432553408976492	26.152693614472426	26.648771239443203	21.76598173710787
33	24.107973249009806	24.991424870775788	26.168734652225183	24.73186722798922
34	24.86685085418526	25.49449911859617	28.540077850227902	21.09857217699067
35	21.351986443616607	27.943829063971314	28.151168009392542	22.55301648301954
36	21.076046464401696	30.12745799039927	28.495026425049957	20.30146912014908
37	22.131853917340873	27.670278175485542	29.268409223938008	20.92945868323558
38	21.259494502531584	28.44502616907595	26.00986011873781	24.28561920965466
39	24.6142898342483	26.180509456533052	25.855763766708705	23.349436942509943
40	22.988684242410798	26.43187593110545	27.50014078570368	23.07929904078007
41	21.58424019235593	29.023527424201827	26.040918298216543	23.35131408522569
42	21.031848285912726	27.791439205320163	28.764652378766442	22.412060130000665
43	22.68441647312188	26.477780602972373	26.524367872190474	24.31343505171528
44	21.555571103606333	24.637668793526245	28.181714240857886	25.625045862009532
45	21.541065909893735	24.522139191838864	27.627786490374522	26.309008407892875
46	24.670604115720728	26.763959542454995	26.62778137089439	21.937654970929884
47	20.594815331819106	26.12436582439842	30.887018192925904	22.393800650856573
48	21.53492253373311	25.809517796166194	28.504241489290898	24.1513181808098
49	21.425195009530764	23.98476442712164	31.60220956762577	22.987830995721822
50	21.668199666551192	24.55541581270894	29.351686100782086	24.42469841995778
51	20.74464545040333	24.008655334412975	27.998266202728	27.2484330124557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	399.0
2	715.0
3	1602.5
4	2490.0
5	1798.5
6	1107.0
7	1042.0
8	977.0
9	998.5
10	1020.0
11	998.0
12	976.0
13	1002.0
14	1028.0
15	1036.5
16	1045.0
17	1100.5
18	1156.0
19	1227.5
20	1299.0
21	1540.0
22	1781.0
23	2073.0
24	2365.0
25	2719.0
26	3895.5
27	4718.0
28	5518.5
29	6319.0
30	7250.5
31	8182.0
32	9506.0
33	10830.0
34	12601.5
35	14373.0
36	16216.0
37	18059.0
38	19650.0
39	21241.0
40	24005.5
41	26770.0
42	30902.0
43	35034.0
44	51734.0
45	68434.0
46	59530.5
47	50627.0
48	51198.5
49	51770.0
50	48036.5
51	44303.0
52	40949.0
53	37595.0
54	35295.5
55	32996.0
56	31355.5
57	29715.0
58	27976.5
59	26238.0
60	24818.0
61	23398.0
62	21853.0
63	20308.0
64	17283.5
65	14259.0
66	11352.0
67	8445.0
68	6907.5
69	5370.0
70	4318.0
71	3266.0
72	2625.0
73	1984.0
74	1650.5
75	1051.0
76	785.0
77	527.0
78	269.0
79	212.0
80	155.0
81	111.0
82	67.0
83	46.5
84	26.0
85	17.0
86	8.0
87	7.5
88	7.0
89	4.5
90	2.0
91	3.5
92	5.0
93	4.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	585997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.68030052427648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1079370880945	23.23828753700274
2	8.703419977498825	5.688607609073066
3	3.3849654543807213	3.318650649403683
4	2.021770323206734	2.6428824701383866
5	1.5555062789641094	2.5417206331973072
6	1.3120302350841297	2.572652542769189
7	1.2413477284999577	2.8397331775754613
8	1.1094861559474745	2.90066728031102
9	1.0352011559516103	3.044761639161933
>10	8.4643800212365	44.03121496588789
>50	0.04193808599047476	0.9162121760191861
>100	0.015202556171535039	0.8966818491550926
>500	0.0031453564492831113	0.7185104461896176
>1k	0.0020969042995220744	1.1211092894289123
>5k	0.0015726782246415557	3.5283077346864986
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	7757	1.323726913277713	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	7024	1.1986409486737988	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	5814	0.9921552499415527	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2223	0.37935347791882895	No Hit
GAACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	1909	0.3257695858511221	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	1352	0.2307179046991708	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC	1060	0.18088829806295936	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	972	0.16587115633697783	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTA	903	0.15409635202910596	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT	680	0.11604154970076638	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	612	0.10443739473068976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09112674638266066	0.0
2	0.0	0.0	0.0	0.6706518975353116	0.0
3	0.0	0.0	0.0	0.8349872098321323	0.0
4	0.0	0.0	0.0	1.3810650907769153	0.0
5	0.0	0.0	0.0	3.3165698800505803	0.0
6	0.0	0.0	0.0	4.019474502429193	0.0
7	0.0	0.0	0.0	4.714529255269225	0.0
8	0.0	0.0	0.0	5.616922953530479	0.0
9	0.0	0.0	0.0	5.9333068258028625	0.0
10	0.0	0.0	0.0	7.602769297453741	0.0
11	0.0	0.0	0.0	8.358404565211085	0.0
12	0.0	0.0	0.0	10.36063324556269	0.0
13	0.0	0.0	0.0	10.735720490036638	0.0
14	0.0	0.0	0.0	10.885038660607478	0.0
15	0.0	0.0	0.0	11.229238374940486	0.0
16	0.0	0.0	0.0	11.650912888632536	0.0
17	1.706493377952447E-4	0.0	0.0	12.09033493345529	0.0
18	1.706493377952447E-4	0.0	0.0	12.575320351469376	0.0
19	1.706493377952447E-4	0.0	0.0	13.016790188345674	0.0
20	1.706493377952447E-4	0.0	0.0	13.345972760952701	0.0
21	1.706493377952447E-4	0.0	0.0	13.737612991192787	0.0
22	1.706493377952447E-4	0.0	0.0	14.183349061513967	0.0
23	1.706493377952447E-4	0.0	0.0	14.554170072543034	0.0
24	1.706493377952447E-4	0.0	0.0	14.870212646139827	0.0
25	1.706493377952447E-4	0.0	0.0	15.163900156485443	0.0
26	1.706493377952447E-4	0.0	0.0	15.449737797292478	0.0
27	1.706493377952447E-4	0.0	0.0	15.76902270830738	0.0
28	1.706493377952447E-4	0.0	0.0	16.051788661034102	0.0
29	1.706493377952447E-4	0.0	0.0	16.35469123562066	0.0
30	1.706493377952447E-4	0.0	0.0	16.75366938738594	0.0
31	1.706493377952447E-4	0.0	0.0	17.070735857009506	0.0
32	1.706493377952447E-4	0.0	0.0	17.38541323590394	0.0
33	1.706493377952447E-4	0.0	0.0	17.694459186651127	0.0
34	1.706493377952447E-4	0.0	0.0	17.992242281103827	0.0
35	1.706493377952447E-4	0.0	0.0	18.31596407490141	0.0
36	1.706493377952447E-4	0.0	0.0	18.63030015512025	0.0
37	1.706493377952447E-4	0.0	0.0	18.93422662573358	0.0
38	3.412986755904894E-4	0.0	0.0	19.277743742715405	0.0
39	3.412986755904894E-4	0.0	0.0	19.638496442814553	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2105556E-7	45.000004	25
TTTCGAC	30	2.1636733E-6	45.000004	24
ATTACGG	30	2.1636733E-6	45.000004	2
CGGTCTA	35	1.2105556E-7	45.000004	31
TCGACGT	30	2.1636733E-6	45.000004	26
TTCGACG	30	2.1636733E-6	45.000004	25
TTTAGCG	25	3.8884573E-5	45.0	1
TTTACGG	40	6.8048394E-9	45.0	2
GCGAATG	25	3.8884573E-5	45.0	1
TAGCGTA	20	7.030453E-4	45.0	25
TACGGGT	95	0.0	42.63158	4
TACGGCT	820	0.0	42.530487	7
GCGTTAG	65	0.0	41.53846	1
CGGCGAA	60	3.6379788E-12	41.250004	31
TTGTACG	55	6.002665E-11	40.909092	1
GCCGATT	55	6.002665E-11	40.909092	9
ACGGGTC	105	0.0	40.714283	5
TTACGGG	150	0.0	40.5	3
CGTTAGG	80	0.0	39.375	2
ACGGTCT	40	3.4543518E-7	39.375	30
>>END_MODULE