FastQCFastQC Report
Sat 18 Jun 2016
SRR3550983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198517
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC48182.4269961766498587No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC45832.308618405476609No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG39111.9701083534407633No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC14960.7535878539369424TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT9550.4810671126402273No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT8340.42011515386591575TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC7460.3757864565755074No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC6630.33397643526750853TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA6440.3244054665343522No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT4510.22718457361334296No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.19696046182442814No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT3590.18084093553700692TruSeq Adapter, Index 15 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC2610.1314748862817794No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATG2440.12291138794158687No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG2340.11787403597676774No Hit
GAATATGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC2220.11182921361898478No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2090.1052806560647199No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2010.10125077449286458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTA207.019175E-445.00000444
CTCGTAG207.019175E-445.0000041
CCGATTT207.019175E-445.00000410
GTCTCGC207.019175E-445.00000431
ACACAAC207.019175E-445.00000411
CGATTGA207.019175E-445.00000410
CACGAGG207.019175E-445.0000042
CTGACTA406.7666406E-945.00000422
TACGGGA207.019175E-445.0000044
GTGGCGA207.019175E-445.00000428
TAACCGG207.019175E-445.0000042
TCGGAAA207.019175E-445.00000421
CTACGAA406.7666406E-945.00000411
CGAGGGC453.8198777E-1045.0000044
TCTCGCG207.019175E-445.00000432
AGCGACT207.019175E-445.00000419
GCTAGTC207.019175E-445.00000427
GCCTAGT207.019175E-445.00000419
GGGCATA453.8198777E-1045.0000047
CATCGAA207.019175E-445.00000442
Produced by FastQC (version 0.11.5)