##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198517
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92600633698877	33.0	31.0	34.0	30.0	34.0
2	32.01300644277316	33.0	31.0	34.0	30.0	34.0
3	31.926827425359036	33.0	31.0	34.0	30.0	34.0
4	35.639129142592324	37.0	35.0	37.0	33.0	37.0
5	33.424265931884925	37.0	35.0	37.0	30.0	37.0
6	34.450334228302864	37.0	35.0	37.0	30.0	37.0
7	35.55657701859287	37.0	35.0	37.0	32.0	37.0
8	35.35098253550074	37.0	35.0	37.0	33.0	37.0
9	37.27775454998816	39.0	37.0	39.0	34.0	39.0
10	37.0543530277004	39.0	37.0	39.0	33.0	39.0
11	36.92949218454843	39.0	37.0	39.0	33.0	39.0
12	36.818458872539885	39.0	35.0	39.0	33.0	39.0
13	36.70090722708886	39.0	35.0	39.0	33.0	39.0
14	37.8613116257046	40.0	36.0	41.0	33.0	41.0
15	37.88740510888236	40.0	36.0	41.0	33.0	41.0
16	37.93037372114227	40.0	36.0	41.0	33.0	41.0
17	37.7843207382743	40.0	36.0	41.0	33.0	41.0
18	37.71384818428648	39.0	36.0	41.0	33.0	41.0
19	37.55303072280963	39.0	36.0	41.0	33.0	41.0
20	37.663409179062754	39.0	36.0	41.0	33.0	41.0
21	37.78969559282076	39.0	36.0	41.0	33.0	41.0
22	37.91224429142088	40.0	36.0	41.0	34.0	41.0
23	38.02361006865911	40.0	36.0	41.0	34.0	41.0
24	37.79673277351562	40.0	35.0	41.0	33.0	41.0
25	37.572228071147556	39.0	35.0	41.0	33.0	41.0
26	37.57669620234035	39.0	35.0	41.0	33.0	41.0
27	37.680002216434865	40.0	35.0	41.0	33.0	41.0
28	37.59322375413692	40.0	35.0	41.0	33.0	41.0
29	37.60990746384441	40.0	36.0	41.0	33.0	41.0
30	37.465743487963245	40.0	36.0	41.0	33.0	41.0
31	37.40176408065808	39.0	35.0	41.0	33.0	41.0
32	37.31435091201257	39.0	35.0	41.0	33.0	41.0
33	37.24290111174358	40.0	35.0	41.0	33.0	41.0
34	37.17007611438819	40.0	35.0	41.0	33.0	41.0
35	37.11876564727454	40.0	35.0	41.0	33.0	41.0
36	37.087932015897884	39.0	35.0	41.0	33.0	41.0
37	37.03865663897802	40.0	35.0	41.0	33.0	41.0
38	36.85188674017842	39.0	35.0	41.0	32.0	41.0
39	36.7976949077409	39.0	35.0	41.0	31.0	41.0
40	36.53477032193717	39.0	35.0	41.0	31.0	41.0
41	36.42801875909872	39.0	35.0	41.0	30.0	41.0
42	36.51174962345794	39.0	35.0	41.0	31.0	41.0
43	36.49574595626571	39.0	35.0	41.0	31.0	41.0
44	36.50521114060761	39.0	35.0	41.0	31.0	41.0
45	36.466191812288116	39.0	35.0	41.0	31.0	41.0
46	36.368532669746166	39.0	35.0	40.0	31.0	41.0
47	36.18064951616234	38.0	35.0	40.0	30.0	41.0
48	36.17749613383236	38.0	35.0	40.0	31.0	41.0
49	36.13128850425908	38.0	35.0	40.0	30.0	41.0
50	36.031710130618535	38.0	35.0	40.0	30.0	41.0
51	34.60381226796697	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	5.0
10	11.0
11	6.0
12	3.0
13	6.0
14	8.0
15	5.0
16	12.0
17	27.0
18	64.0
19	122.0
20	214.0
21	429.0
22	620.0
23	722.0
24	947.0
25	1220.0
26	1546.0
27	1688.0
28	1899.0
29	2355.0
30	2881.0
31	3946.0
32	5478.0
33	8163.0
34	14654.0
35	19042.0
36	15113.0
37	23023.0
38	38566.0
39	55718.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.11737533813225	17.692691306034245	21.228912385337274	13.961020970496229
2	26.65061430507211	32.91405773812822	24.124886029911796	16.310441926887872
3	28.984419470372814	31.67940277155105	24.503694897666193	14.83248286040994
4	26.383130915740217	22.362820312618062	34.2207468378023	17.03330193383942
5	27.974430401426574	30.874937662769437	22.03337749411889	19.117254441685095
6	23.644826387664533	37.286982978787705	24.834145186558327	14.234045446989427
7	73.24108262768428	5.392485278338883	16.554249761985123	4.8121823319917185
8	72.7781499821174	11.346131565558618	9.921568429907767	5.954150022416217
9	68.67724174755814	5.4811426729197	10.73862691860143	15.102988660920728
10	45.705405582393446	18.913745422306402	18.680012291138794	16.700836704161357
11	34.323508817884615	21.088370265518822	23.277099694232735	21.311021222363827
12	27.448530856299456	18.770180891309057	30.37825475903827	23.403033493353213
13	22.62274767400273	23.498239445488295	35.289169189540445	18.589843690968532
14	19.52880609721082	29.821627366925757	28.489751507427574	22.159815028435855
15	16.039936126377086	22.35677549026028	41.63421772442663	19.969070658936012
16	18.53695149533793	23.62366950941229	31.467833989028648	26.37154500622113
17	19.65826604270667	22.847917306830144	31.33031428038909	26.1635023700741
18	20.872771601424564	23.622158303822847	30.826579083907173	24.67849101084542
19	22.68319589758056	25.271387337104628	26.997184120251667	25.048232645063145
20	24.336958547630683	24.468433433912463	31.073913065379795	20.120694953077066
21	22.172912143544384	29.47656875733564	27.41881047970703	20.931708619412948
22	19.636101694061466	22.860510686742195	28.582942518776726	28.920445100419613
23	19.54442188830176	29.3330042263383	28.398071701667867	22.724502183692078
24	23.950593651929054	23.84178684948896	27.27020859674486	24.937410901837122
25	18.392379493947622	31.533319564571293	27.303455119712673	22.77084582176841
26	17.66548960542422	24.646251958270575	32.31914647108308	25.369111965222125
27	21.875204642423572	28.141166751462094	27.351813698574933	22.631814907539404
28	16.394565704700355	25.205901761561982	34.033357344711035	24.36617518902663
29	20.344857115511516	21.836920767490945	31.114211881098342	26.704010235899194
30	18.82911790929744	29.545076744057187	28.778391775011713	22.847413571633663
31	23.78385730189354	23.43930242749991	24.431660764569283	28.345179506037265
32	23.343592740168347	27.29086173980062	26.01087060554008	23.35467491449095
33	21.949757451502894	28.671096178161065	22.99702292498879	26.38212344534725
34	23.996433554808906	22.998030395381754	28.440385458172347	24.56515059163699
35	17.930454318773705	27.15586070714347	29.145110998050544	25.76857397603228
36	21.112549554949954	27.866631069379448	28.940090773082407	22.08072860258819
37	20.143866772115235	26.67680853528917	30.103719076955628	23.075605615639972
38	18.622082743543373	28.65447291667716	25.1691290922188	27.554315247560663
39	24.451306437232077	24.473974521073764	26.965448802873304	24.109270238820855
40	20.698479223441822	26.08995703138774	30.569170398504912	22.642393346665525
41	20.696464282655892	27.71097689366654	24.59436723303294	26.998191590644634
42	19.61343361021978	24.42309726622909	32.27632897938212	23.687140144169014
43	21.349305097296455	27.944206289637663	25.628535591410305	25.077953021655574
44	20.746837802304086	24.85076844804223	28.5169532080376	25.885440541616084
45	18.97671232186664	23.49672823989885	27.884261801256315	29.642297636978192
46	26.203801185792653	26.294473521159397	26.330742455306094	21.170982837741857
47	19.398842416518487	23.58135575290781	34.643884402847114	22.375917427726595
48	22.118508742324337	24.896104615725605	26.350388127968888	26.634998513981174
49	19.889984233088352	20.81131590745377	34.26910541666457	25.02959444279331
50	22.25099109899908	22.48522796536317	30.492098913443183	24.77168202219457
51	20.961932731201863	22.46004120553907	25.628535591410305	30.949490471848755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	129.0
2	193.0
3	524.5
4	856.0
5	635.0
6	414.0
7	407.0
8	400.0
9	392.0
10	384.0
11	399.0
12	414.0
13	400.0
14	386.0
15	358.0
16	330.0
17	338.5
18	347.0
19	340.5
20	334.0
21	362.5
22	391.0
23	473.5
24	556.0
25	677.0
26	980.5
27	1163.0
28	1250.0
29	1337.0
30	1601.5
31	1866.0
32	2463.0
33	3060.0
34	3576.0
35	4092.0
36	4431.5
37	4771.0
38	5199.0
39	5627.0
40	6842.5
41	8058.0
42	10295.0
43	12532.0
44	22642.5
45	32753.0
46	26651.5
47	20550.0
48	20109.5
49	19669.0
50	17134.5
51	14600.0
52	13931.0
53	13262.0
54	12310.5
55	11359.0
56	10243.5
57	9128.0
58	8343.0
59	7558.0
60	6769.0
61	5980.0
62	5471.5
63	4963.0
64	4432.5
65	3902.0
66	3133.0
67	2364.0
68	1919.5
69	1475.0
70	1315.5
71	1156.0
72	942.5
73	729.0
74	512.5
75	235.5
76	175.0
77	144.5
78	114.0
79	89.5
80	65.0
81	44.0
82	23.0
83	16.0
84	9.0
85	7.0
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	198517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.01202919649199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44397817876626	26.808787156767433
2	9.2656315568611	6.673483882992389
3	3.2172331794656595	3.4757728557252023
4	1.716323961393202	2.472332344333231
5	1.2449293607497554	2.2416216243445146
6	1.039306196670863	2.2456515059163698
7	0.8770457406630298	2.210893777359118
8	0.8140998741082669	2.345391074819789
9	0.7581479927262554	2.4572202884387733
>10	6.551965309833544	36.94847292675186
>50	0.032172331794656596	0.7470392963826775
>100	0.026577143656455447	1.9786718517809558
>500	0.006993985172751433	1.9353506248835113
>1k	0.005595188138201147	7.459310789504174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	4818	2.4269961766498587	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	4583	2.308618405476609	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	3911	1.9701083534407633	No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1496	0.7535878539369424	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	955	0.4810671126402273	No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	834	0.42011515386591575	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC	746	0.3757864565755074	No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	663	0.33397643526750853	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	644	0.3244054665343522	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT	451	0.22718457361334296	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.19696046182442814	No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT	359	0.18084093553700692	TruSeq Adapter, Index 15 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	261	0.1314748862817794	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATG	244	0.12291138794158687	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	234	0.11787403597676774	No Hit
GAATATGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	222	0.11182921361898478	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	209	0.1052806560647199	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	201	0.10125077449286458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20199781378924728	0.0
2	0.0	0.0	0.0	1.5117093246422222	0.0
3	0.0	0.0	0.0	1.8285587632293456	0.0
4	0.0	0.0	0.0	2.6687890709611772	0.0
5	0.0	0.0	0.0	6.099729494199489	0.0
6	0.0	0.0	0.0	6.996378143937295	0.0
7	0.0	0.0	0.0	8.278887954180247	0.0
8	0.0	0.0	0.0	9.961363510429837	0.0
9	0.0	0.0	0.0	10.487263055556955	0.0
10	0.0	0.0	0.0	13.639637915140769	0.0
11	0.0	0.0	0.0	14.864721912984782	0.0
12	0.0	0.0	0.0	18.755572570611083	0.0
13	0.0	0.0	0.0	19.41345073721646	0.0
14	0.0	0.0	0.0	19.659777248296116	0.0
15	0.0	0.0	0.0	20.352413143458747	0.0
16	0.0	0.0	0.0	21.0248996307621	0.0
17	0.0	0.0	0.0	21.787554718235718	0.0
18	0.0	0.0	0.0	22.703849040636317	0.0
19	0.0	0.0	0.0	23.509321619810898	0.0
20	0.0	0.0	0.0	24.07904612703194	0.0
21	0.0	0.0	0.0	24.65481545661077	0.0
22	0.0	0.0	0.0	25.30816000644781	0.0
23	0.0	0.0	0.0	25.87687704327589	0.0
24	0.0	0.0	0.0	26.328727514520168	0.0
25	0.0	0.0	0.0	26.71660361581124	0.0
26	0.0	0.0	0.0	27.127651536140483	0.0
27	0.0	0.0	0.0	27.562375010704372	0.0
28	0.0	0.0	0.0	27.944710024834144	0.0
29	0.0	0.0	0.0	28.342660830054857	0.0
30	0.0	0.0	0.0	28.834306381821204	0.0
31	0.0	0.0	0.0	29.244346831757483	0.0
32	0.0	0.0	0.0	29.655898487283206	0.0
33	0.0	0.0	0.0	30.078028581935047	0.0
34	0.0	0.0	0.0	30.461371066457783	0.0
35	0.0	0.0	0.0	30.896094541021675	0.0
36	0.0	0.0	0.0	31.285481847902194	0.0
37	0.0	0.0	0.0	31.701567120196255	0.0
38	0.0	0.0	0.0	32.13427565397422	0.0
39	0.0	0.0	0.0	32.53726381115975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTA	20	7.019175E-4	45.000004	44
CTCGTAG	20	7.019175E-4	45.000004	1
CCGATTT	20	7.019175E-4	45.000004	10
GTCTCGC	20	7.019175E-4	45.000004	31
ACACAAC	20	7.019175E-4	45.000004	11
CGATTGA	20	7.019175E-4	45.000004	10
CACGAGG	20	7.019175E-4	45.000004	2
CTGACTA	40	6.7666406E-9	45.000004	22
TACGGGA	20	7.019175E-4	45.000004	4
GTGGCGA	20	7.019175E-4	45.000004	28
TAACCGG	20	7.019175E-4	45.000004	2
TCGGAAA	20	7.019175E-4	45.000004	21
CTACGAA	40	6.7666406E-9	45.000004	11
CGAGGGC	45	3.8198777E-10	45.000004	4
TCTCGCG	20	7.019175E-4	45.000004	32
AGCGACT	20	7.019175E-4	45.000004	19
GCTAGTC	20	7.019175E-4	45.000004	27
GCCTAGT	20	7.019175E-4	45.000004	19
GGGCATA	45	3.8198777E-10	45.000004	7
CATCGAA	20	7.019175E-4	45.000004	42
>>END_MODULE