FastQCFastQC Report
Sat 18 Jun 2016
SRR3550982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535991
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC37480.6992654727411468No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC23600.4403059006587797No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG22130.4128800670160506No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC11980.22351121567339752No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.20746617014091653No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT9450.1763089305604012No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT5490.10242709299223308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG207.029912E-445.00000431
CTAGGCG253.8880098E-545.01
AATTGCG302.163326E-644.9999961
ATAGCGG451.9250365E-840.02
CGCTAGG451.9250365E-840.02
TAGAACG356.2420495E-638.5714261
TCTCGGT356.2420495E-638.57142612
GTTCACG356.2420495E-638.5714261
GCGCTAG356.2420495E-638.5714261
TACGGCT2850.037.1052637
CAACGAG1900.036.71052614
CGTTTTT8700.036.2068981
CTATGCG504.870708E-836.01
CTCGTAC250.002106107536.029
TACGGTT250.002106107536.033
GCGTTAG504.870708E-836.01
TATGACG250.002106107536.01
CTACGAA504.870708E-836.011
TGCGAAG250.002106107536.01
GGACGTA250.002106107536.08
Produced by FastQC (version 0.11.5)