##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535991
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04877134130984	33.0	31.0	34.0	30.0	34.0
2	32.1304145032286	33.0	31.0	34.0	30.0	34.0
3	32.03321884136114	33.0	31.0	34.0	30.0	34.0
4	35.73670453421793	37.0	35.0	37.0	33.0	37.0
5	33.442947736062735	37.0	35.0	37.0	30.0	37.0
6	34.509368627458294	37.0	35.0	37.0	30.0	37.0
7	35.670981415732726	37.0	35.0	37.0	32.0	37.0
8	35.56777072749356	37.0	35.0	37.0	33.0	37.0
9	37.51566537497831	39.0	37.0	39.0	35.0	39.0
10	37.16096165793829	39.0	37.0	39.0	34.0	39.0
11	37.04119472155316	39.0	37.0	39.0	34.0	39.0
12	36.97652012813648	39.0	37.0	39.0	33.0	39.0
13	37.03878609902032	39.0	37.0	39.0	33.0	39.0
14	38.20072725101728	40.0	37.0	41.0	33.0	41.0
15	38.30048825446696	40.0	37.0	41.0	34.0	41.0
16	38.12856559158642	40.0	37.0	41.0	34.0	41.0
17	38.060168920746804	40.0	37.0	41.0	33.0	41.0
18	37.938590386778884	40.0	36.0	41.0	33.0	41.0
19	37.76558561617639	39.0	36.0	41.0	33.0	41.0
20	38.02100035261786	40.0	36.0	41.0	34.0	41.0
21	37.982619111141794	40.0	36.0	41.0	34.0	41.0
22	38.119640068583244	40.0	36.0	41.0	34.0	41.0
23	38.18839868579883	40.0	37.0	41.0	34.0	41.0
24	38.088568651339294	40.0	36.0	41.0	34.0	41.0
25	37.73721387112843	40.0	36.0	41.0	33.0	41.0
26	37.82817248797088	40.0	36.0	41.0	34.0	41.0
27	37.90437152862641	40.0	36.0	41.0	34.0	41.0
28	37.7967074074005	40.0	36.0	41.0	33.0	41.0
29	37.78678000190302	40.0	36.0	41.0	33.0	41.0
30	37.583179568313646	40.0	36.0	41.0	33.0	41.0
31	37.52132405208297	40.0	35.0	41.0	33.0	41.0
32	37.495735935864595	40.0	35.0	41.0	33.0	41.0
33	37.429859829735946	40.0	35.0	41.0	33.0	41.0
34	37.31734301508794	40.0	35.0	41.0	33.0	41.0
35	37.31277017711118	40.0	35.0	41.0	33.0	41.0
36	37.2038504377872	40.0	35.0	41.0	33.0	41.0
37	37.19937088495889	40.0	35.0	41.0	33.0	41.0
38	37.070598200342914	40.0	35.0	41.0	32.0	41.0
39	36.981805664647354	39.0	35.0	41.0	32.0	41.0
40	36.85451248248572	39.0	35.0	41.0	31.0	41.0
41	36.731547731211904	39.0	35.0	41.0	31.0	41.0
42	36.76850170991677	39.0	35.0	41.0	31.0	41.0
43	36.730502937549325	39.0	35.0	41.0	31.0	41.0
44	36.66274433712506	39.0	35.0	41.0	31.0	41.0
45	36.61904397648468	39.0	35.0	41.0	31.0	41.0
46	36.52496590427825	39.0	35.0	41.0	31.0	41.0
47	36.427995992470024	39.0	35.0	41.0	31.0	41.0
48	36.37597832799431	39.0	35.0	41.0	31.0	41.0
49	36.305001389948714	39.0	35.0	41.0	31.0	41.0
50	36.2269776917896	38.0	35.0	40.0	31.0	41.0
51	34.975148836454345	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	14.0
9	38.0
10	32.0
11	15.0
12	17.0
13	10.0
14	20.0
15	19.0
16	44.0
17	77.0
18	143.0
19	288.0
20	533.0
21	898.0
22	1270.0
23	1745.0
24	2273.0
25	3128.0
26	4112.0
27	4548.0
28	5285.0
29	6268.0
30	7536.0
31	9885.0
32	13091.0
33	19295.0
34	35504.0
35	44515.0
36	39817.0
37	62764.0
38	108114.0
39	164652.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27348780110114	18.77512868686228	22.92743721443084	15.023946297605745
2	29.899009498293815	26.905115944111003	25.48979367190867	17.70608088568651
3	31.785421770141664	26.070400435828212	26.236448000059703	15.907729793970422
4	28.378834719239688	25.02280821879472	27.89785649385904	18.700500568106555
5	24.953217498055004	34.096468037709585	23.091992216287213	17.858322247948195
6	26.19894737038495	35.36141465061913	24.226899332264907	14.212738646731008
7	80.14518900503927	6.003645583601217	9.672737042226455	4.178428369133064
8	82.27432923314011	6.246933250744882	7.583522857659923	3.8952146584550857
9	76.05780694078818	6.912429499749064	9.45127809981884	7.578485459643912
10	44.064359289614934	23.568119613948742	17.436673376978344	14.930847719457976
11	34.14460317430703	24.721497189318477	23.020162651984826	18.113736984389664
12	29.759454916220605	23.472409051644526	26.57451337802314	20.193622654111728
13	26.09819941006472	23.6474119901267	29.519152373827172	20.735236225981406
14	22.60373774932788	26.774516736288483	28.980150786113946	21.64159472826969
15	22.825569832329272	24.953777208945674	32.48002298546058	19.740629973264475
16	26.899891975798102	25.672259422266418	28.02509743633755	19.402751165597927
17	25.757335477647946	25.95174172700661	27.773040965240085	20.517881830105356
18	27.06276784498247	25.642035034170352	27.442251828855333	19.852945291991844
19	25.793530115244472	27.898789345343484	25.728417081630102	20.57926345778194
20	28.160174331285415	27.577515294100085	26.344658772255503	17.917651602358994
21	26.92825066092528	28.556263071581427	26.58029705722671	17.935189210266593
22	25.439606262045444	24.951165224789225	28.249914644089174	21.35931386907616
23	25.46497982242239	26.2051041901823	28.07920282243545	20.250713164959862
24	25.852672899358385	24.447985134078746	27.781063488006332	21.918278478556545
25	23.564761348604733	27.673785567294974	26.25622445152997	22.505228632570322
26	22.20093247834385	26.44689929495085	27.88703541663946	23.465132810065843
27	23.052812453940458	26.523766257269244	27.437774141729992	22.985647147060305
28	21.205579944439364	28.196928679772608	28.240959269838484	22.35653210594954
29	23.767563261323417	28.182562766912135	27.007916177697012	21.041957794067436
30	24.754146991274105	26.44932470881041	28.196928679772608	20.599599620142875
31	26.398204447462735	25.820582808293423	25.892972083486477	21.88824066075736
32	26.31256868119054	27.930319725517776	25.471882923407296	20.285228669884383
33	25.611064364886726	27.537402680268887	25.192214048370214	21.659318906474176
34	23.741816560352692	27.928827163142667	27.637590929698447	20.691765346806196
35	23.026879182672843	28.90011212874843	26.428801976152585	21.644206712426143
36	23.56849275454252	31.304070404167234	24.44070889250006	20.686727948790185
37	24.988665854463974	29.100115487013774	25.31591015520783	20.59530850331442
38	22.94366883026021	30.184462052534467	23.7696155345892	23.102253582616125
39	25.456211018468593	28.01987346802465	24.77746827838527	21.746447235121487
40	24.001335843325727	26.87246614215537	26.00901880815163	23.117179206367272
41	23.053185594534238	27.510909698110602	25.50957012337894	23.926334583976224
42	22.606909444375	26.305852150502528	26.583468752273827	24.503769652848646
43	23.830624021672005	25.81125429344896	25.70770777867539	24.65041390620365
44	22.3833982287016	26.334957116817257	26.662201417561114	24.61944323692002
45	23.417370814062178	25.55789183027327	26.137752313005258	24.886985042659298
46	23.779130619730555	27.912408977016405	25.50994326397272	22.798517139280325
47	22.233022569408813	26.665186542311343	28.05159041849583	23.050200469784006
48	22.504668921679656	25.747074111319034	28.037970786822914	23.7102861801784
49	23.56849275454252	24.67205606064281	29.215415930491368	22.5440352543233
50	22.254105012957307	25.747447251912813	28.040209630385586	23.958238104744296
51	21.854658007317287	24.685115981425064	27.809608743430392	25.650617267827258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	72.0
1	349.0
2	626.0
3	1803.5
4	2981.0
5	2041.0
6	1101.0
7	1101.5
8	1102.0
9	1040.5
10	979.0
11	1017.5
12	1056.0
13	1074.5
14	1093.0
15	1141.5
16	1190.0
17	1156.5
18	1123.0
19	1165.0
20	1207.0
21	1319.0
22	1431.0
23	1636.0
24	1841.0
25	2322.5
26	3451.0
27	4098.0
28	4838.5
29	5579.0
30	6791.5
31	8004.0
32	9317.0
33	10630.0
34	12212.0
35	13794.0
36	15059.5
37	16325.0
38	17903.0
39	19481.0
40	21574.5
41	23668.0
42	25633.0
43	27598.0
44	29508.0
45	31418.0
46	32914.0
47	34410.0
48	42741.5
49	51073.0
50	48170.0
51	45267.0
52	45711.0
53	46155.0
54	42848.5
55	39542.0
56	36042.5
57	32543.0
58	30362.0
59	28181.0
60	26993.5
61	25806.0
62	22740.5
63	19675.0
64	16409.5
65	13144.0
66	10636.0
67	8128.0
68	6608.5
69	5089.0
70	4219.0
71	3349.0
72	2700.0
73	2051.0
74	1608.0
75	883.5
76	602.0
77	472.0
78	342.0
79	249.0
80	156.0
81	107.0
82	58.0
83	41.5
84	25.0
85	20.0
86	15.0
87	10.0
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.62400395127253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.14527990943657	24.63231724705613
2	8.673334169909673	6.179577814791385
3	3.9397865107629744	4.210529106797711
4	2.6839627693288306	3.8245400119855444
5	2.163302279019326	3.853274446779066
6	1.803928456505495	3.8557892677418817
7	1.6369111110410097	4.081932952132478
8	1.382946511814193	3.94128736010139
9	1.2581555748659803	4.033848525330711
>10	7.271454790731441	37.695164157640164
>50	0.02624225422092617	0.6001763758918954
>100	0.01049690168833186	0.7263816885772848
>500	0.0015745352532497792	0.3774941130598553
>1k	0.002624225422082965	1.9876869321145727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC	3748	0.6992654727411468	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC	2360	0.4403059006587797	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG	2213	0.4128800670160506	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1198	0.22351121567339752	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.20746617014091653	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	945	0.1763089305604012	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	549	0.10242709299223308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8657029688931345E-4	0.0	0.0	0.07612068113083989	0.0
2	1.8657029688931345E-4	0.0	0.0	0.44944784520635606	0.0
3	1.8657029688931345E-4	0.0	0.0	0.5987040827178068	0.0
4	1.8657029688931345E-4	0.0	0.0	0.9403142963221397	0.0
5	1.8657029688931345E-4	0.0	0.0	2.0813782320971805	0.0
6	1.8657029688931345E-4	0.0	0.0	2.7175829444897395	0.0
7	1.8657029688931345E-4	0.0	0.0	3.2265467144037867	0.0
8	1.8657029688931345E-4	0.0	0.0	3.9317824366453915	0.0
9	1.8657029688931345E-4	0.0	0.0	4.286079430438198	0.0
10	1.8657029688931345E-4	0.0	0.0	5.291320190077818	0.0
11	3.731405937786269E-4	0.0	0.0	5.929390605439271	0.0
12	3.731405937786269E-4	0.0	0.0	6.958885503674502	0.0
13	3.731405937786269E-4	0.0	0.0	7.247136612368491	0.0
14	3.731405937786269E-4	0.0	0.0	7.384452350879026	0.0
15	3.731405937786269E-4	0.0	0.0	7.613000964568435	0.0
16	3.731405937786269E-4	0.0	0.0	7.922894227701584	0.0
17	3.731405937786269E-4	0.0	0.0	8.272340393775268	0.0
18	3.731405937786269E-4	0.0	0.0	8.670481407337064	0.0
19	3.731405937786269E-4	0.0	0.0	8.996792856596473	0.0
20	3.731405937786269E-4	0.0	0.0	9.294559050431817	0.0
21	3.731405937786269E-4	0.0	0.0	9.623109343253898	0.0
22	3.731405937786269E-4	0.0	0.0	10.001473905345426	0.0
23	3.731405937786269E-4	0.0	0.0	10.338419861527525	0.0
24	3.731405937786269E-4	0.0	0.0	10.61715588508016	0.0
25	3.731405937786269E-4	0.0	0.0	10.855033013614035	0.0
26	3.731405937786269E-4	0.0	0.0	11.108582047086612	0.0
27	3.731405937786269E-4	0.0	0.0	11.373885009263216	0.0
28	3.731405937786269E-4	0.0	0.0	11.617732387297547	0.0
29	3.731405937786269E-4	0.0	0.0	11.876691959379915	0.0
30	3.731405937786269E-4	0.0	0.0	12.219421594765583	0.0
31	5.597108906679403E-4	0.0	0.0	12.493679931192874	0.0
32	5.597108906679403E-4	0.0	0.0	12.779505626027303	0.0
33	5.597108906679403E-4	0.0	0.0	13.072234421846636	0.0
34	5.597108906679403E-4	0.0	0.0	13.350037593914823	0.0
35	5.597108906679403E-4	0.0	0.0	13.624109360045225	0.0
36	5.597108906679403E-4	0.0	0.0	13.915718734083221	0.0
37	5.597108906679403E-4	0.0	0.0	14.216283482371905	0.0
38	5.597108906679403E-4	0.0	0.0	14.588304654369196	0.0
39	5.597108906679403E-4	0.0	0.0	14.937004539255323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCCG	20	7.029912E-4	45.000004	31
CTAGGCG	25	3.8880098E-5	45.0	1
AATTGCG	30	2.163326E-6	44.999996	1
ATAGCGG	45	1.9250365E-8	40.0	2
CGCTAGG	45	1.9250365E-8	40.0	2
TAGAACG	35	6.2420495E-6	38.571426	1
TCTCGGT	35	6.2420495E-6	38.571426	12
GTTCACG	35	6.2420495E-6	38.571426	1
GCGCTAG	35	6.2420495E-6	38.571426	1
TACGGCT	285	0.0	37.105263	7
CAACGAG	190	0.0	36.710526	14
CGTTTTT	870	0.0	36.206898	1
CTATGCG	50	4.870708E-8	36.0	1
CTCGTAC	25	0.0021061075	36.0	29
TACGGTT	25	0.0021061075	36.0	33
GCGTTAG	50	4.870708E-8	36.0	1
TATGACG	25	0.0021061075	36.0	1
CTACGAA	50	4.870708E-8	36.0	11
TGCGAAG	25	0.0021061075	36.0	1
GGACGTA	25	0.0021061075	36.0	8
>>END_MODULE