FastQCFastQC Report
Sat 18 Jun 2016
SRR3550979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418851
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC63161.5079348025908974No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC56081.3389009456823548No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG51671.2336129076927116No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC20150.4810779967100473No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT13200.31514786881253715No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT11950.28530432062953176No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC8980.2143960501467109No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.17571881170153586No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA7330.17500256654514373No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC7210.1721375859195752No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT5630.13441534101625638No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT4580.10934676054253183No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG207.0281466E-445.01
GATCGCG207.0281466E-445.021
CGTTATT253.886545E-545.01
CACGTTG207.0281466E-445.027
CACGTAG253.886545E-545.01
GACCTAA253.886545E-545.09
TCAACGG253.886545E-545.02
CTAGCGG551.8189894E-1245.02
TAATACG800.042.18754
CGGGTGC403.451205E-739.3756
TACGGCT7950.039.3396227
TCTACGG356.2387826E-638.571432
CGCTAGG356.2387826E-638.571432
ATAACGG356.2387826E-638.571432
TATGGGT1350.038.3333324
CGATGAA3350.037.61194219
CGTTTTT5450.037.568811
TCGATAG301.13868366E-437.4999961
TATCGAG301.13868366E-437.4999961
GTACGGA301.13868366E-437.49999617