SRR3550978_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254976
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	2123	0.8326273845381525	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	2075	0.8138020833333334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	1936	0.7592871485943775	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	840	0.3294427710843374	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	534	0.20943147590361447	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	517	0.20276418172690763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.1717808734939759	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	410	0.16079944779116467	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	338	0.13256149598393574	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTA	322	0.12628639558232932	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	288	0.11295180722891565	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAC	20	7.022951E-4	45.0	17
TTTACGG	20	7.022951E-4	45.0	2
TAACGCG	20	7.022951E-4	45.0	1
TTAAGCG	20	7.022951E-4	45.0	1
CGTAAGG	25	3.8822407E-5	45.0	2
TAGGACG	20	7.022951E-4	45.0	1
TACACGG	20	7.022951E-4	45.0	2
TGCACGG	20	7.022951E-4	45.0	2
CACAACG	170	0.0	43.676468	12
TACGGGA	100	0.0	42.75	4
TAGTAGG	65	0.0	41.53846	2
ACAACGA	180	0.0	41.25	13
ACGACCA	140	0.0	40.17857	28
AGCAACG	40	3.444129E-7	39.375	10
CGCGCAA	40	3.444129E-7	39.375	18
AATGGGC	80	0.0	39.375	4
AACGACG	40	3.444129E-7	39.375	13
CAACGAG	190	0.0	39.078945	14
ACGGGCG	75	0.0	39.0	5
AGGGATC	75	0.0	39.0	6