##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254976
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04286677961847	33.0	31.0	34.0	30.0	34.0
2	32.12549416415663	33.0	31.0	34.0	30.0	34.0
3	32.0181703376004	33.0	31.0	34.0	30.0	34.0
4	35.72243269954819	37.0	35.0	37.0	33.0	37.0
5	33.524578783885545	37.0	35.0	37.0	31.0	37.0
6	34.54945171310241	37.0	35.0	37.0	30.0	37.0
7	35.67804813002008	37.0	35.0	37.0	32.0	37.0
8	35.513193398594375	37.0	35.0	37.0	33.0	37.0
9	37.42443210341366	39.0	37.0	39.0	35.0	39.0
10	37.16788638930723	39.0	37.0	39.0	34.0	39.0
11	37.095224648594375	39.0	37.0	39.0	34.0	39.0
12	37.07802302961847	39.0	37.0	39.0	34.0	39.0
13	37.10826509161647	39.0	37.0	39.0	33.0	39.0
14	38.31696708709839	40.0	38.0	41.0	34.0	41.0
15	38.40360661395582	40.0	38.0	41.0	34.0	41.0
16	38.27820657630522	40.0	37.0	41.0	34.0	41.0
17	38.183248619477915	40.0	37.0	41.0	34.0	41.0
18	38.07220679593374	40.0	37.0	41.0	34.0	41.0
19	37.89053871736948	40.0	37.0	41.0	33.0	41.0
20	38.09163607555221	40.0	37.0	41.0	34.0	41.0
21	38.096691453313255	40.0	37.0	41.0	34.0	41.0
22	38.23124921561245	40.0	37.0	41.0	34.0	41.0
23	38.286493630773094	40.0	37.0	41.0	34.0	41.0
24	38.161348519076306	40.0	37.0	41.0	34.0	41.0
25	37.843302899096386	40.0	36.0	41.0	33.0	41.0
26	37.894366528614455	40.0	36.0	41.0	34.0	41.0
27	37.94319857555221	40.0	36.0	41.0	34.0	41.0
28	37.83036050451807	40.0	36.0	41.0	33.0	41.0
29	37.83052130396587	40.0	36.0	41.0	33.0	41.0
30	37.64233104292169	40.0	36.0	41.0	33.0	41.0
31	37.55476593875502	40.0	36.0	41.0	33.0	41.0
32	37.50402390813253	40.0	36.0	41.0	33.0	41.0
33	37.413564413905625	40.0	36.0	41.0	33.0	41.0
34	37.34007122238956	40.0	36.0	41.0	33.0	41.0
35	37.323144139056225	40.0	36.0	41.0	33.0	41.0
36	37.188158885542165	40.0	35.0	41.0	32.0	41.0
37	37.141103476405625	40.0	35.0	41.0	32.0	41.0
38	36.908928683483936	39.0	35.0	41.0	31.0	41.0
39	36.823057856425706	39.0	35.0	41.0	31.0	41.0
40	36.64969252008032	39.0	35.0	41.0	31.0	41.0
41	36.56499827434739	39.0	35.0	41.0	31.0	41.0
42	36.60713165160642	39.0	35.0	41.0	31.0	41.0
43	36.528018323293175	39.0	35.0	41.0	31.0	41.0
44	36.47065213980924	39.0	35.0	41.0	31.0	41.0
45	36.392589890813255	39.0	35.0	41.0	31.0	41.0
46	36.238108684738954	39.0	35.0	41.0	30.0	41.0
47	36.0843608810241	38.0	35.0	40.0	30.0	41.0
48	36.015687751004016	38.0	35.0	40.0	30.0	41.0
49	35.94470067771084	38.0	35.0	40.0	30.0	41.0
50	35.84965251631526	38.0	35.0	40.0	30.0	41.0
51	34.4373078250502	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	16.0
10	22.0
11	9.0
12	11.0
13	12.0
14	13.0
15	13.0
16	18.0
17	41.0
18	83.0
19	173.0
20	316.0
21	481.0
22	664.0
23	869.0
24	1138.0
25	1533.0
26	1989.0
27	2315.0
28	2580.0
29	3076.0
30	3634.0
31	4769.0
32	6221.0
33	9084.0
34	16089.0
35	20274.0
36	20120.0
37	31172.0
38	52079.0
39	76134.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77019013554217	17.378106174698797	20.64468812751004	16.207015562248998
2	29.798883032128515	27.924196787148592	24.918815888554217	17.358104292168676
3	31.963792670682732	27.055487575301207	25.01960968875502	15.961110065261044
4	27.79281187248996	25.102362575301207	28.241481551204817	18.863344001004016
5	26.284826807228917	32.24970193273092	22.455838980923694	19.009632279116463
6	24.78233245481928	36.64933170180723	23.666148970883537	14.902186872489958
7	78.63485190763052	5.385604919678714	11.599915286144578	4.379627886546185
8	78.6128890562249	7.9450614959839365	8.5933578062249	4.848691641566265
9	72.7468467620482	7.053997238955824	10.77199422690763	9.427161772088354
10	41.283885542168676	25.27139809236948	17.229464733935743	16.215251631526105
11	32.316374874497996	25.57417168674699	23.335529618473895	18.773923820281123
12	27.979888303212853	23.254345507028113	27.253937625502004	21.51182856425703
13	24.774488579317268	25.141974146586342	30.552679467871485	19.5308578062249
14	20.775288654618475	29.624356802208833	27.195108559236946	22.405245983935743
15	19.65126129518072	25.332580321285143	34.395394076305216	20.620764307228914
16	22.278959588353413	25.116481551204817	29.560037023092367	23.044521837349397
17	21.92480860943775	24.750564759036145	28.744666164658632	24.57996046686747
18	25.389448418674696	24.114818649598394	27.249231300200805	23.246501631526105
19	24.100699673694777	26.83429028614458	25.903614457831324	23.161395582329316
20	28.014401355421686	24.685068398594378	27.04097640562249	20.25955384036145
21	25.686339106425706	27.467291039156628	25.93734312248996	20.90902673192771
22	24.115603037148595	23.75949108935743	26.469550075301207	25.65535579819277
23	23.695171310240966	28.3195281124498	26.305613077309236	21.6796875
24	23.499858810240966	25.040788152610443	27.4394452811245	24.019907756024097
25	22.947649974899598	28.44581450803213	26.185993975903614	22.42054154116466
26	22.66487826305221	26.316202309236946	27.578674071285143	23.440245356425706
27	25.045494477911646	26.137754141566266	26.09147527610442	22.72527610441767
28	21.029038027108435	28.230892319277107	28.783885542168676	21.956184111445783
29	23.66261922690763	25.825567896586342	28.073622615461847	22.438190261044177
30	24.542702058232933	25.22747238955823	28.900759287148592	21.32906626506024
31	25.79929091365462	26.596620858433734	24.0242218875502	23.57986634036145
32	26.822916666666668	27.0511734437751	25.702811244979916	20.423098644578314
33	24.047753514056225	26.04598079819277	25.6816327811245	24.224632906626507
34	23.263365963855424	26.39385667670683	27.769672439759034	22.573104919678716
35	25.33650225903614	24.91489395080321	26.386404994979916	23.36219879518072
36	23.921467118473895	29.28510918674699	26.296592620481928	20.49683107429719
37	25.62437248995984	26.68055032630522	25.94714796686747	21.74792921686747
38	23.549667419678716	28.41796875	25.20511734437751	22.827246485943775
39	25.95695281124498	24.334054969879517	25.936166541164656	23.772825677710845
40	25.36905434236948	24.38621674196787	28.980766817269078	21.263962098393574
41	21.33377259036145	27.162948669678716	26.821347891566266	24.681930848393574
42	25.17413403614458	26.684472264056225	26.720946285140563	21.420447414658632
43	24.798412399598394	26.404053714859437	26.0746109437751	22.722922941767067
44	23.942645582329316	26.52524159136546	26.434252635542173	23.097860190763054
45	23.835968875502008	24.049322289156628	26.499748995983936	25.614959839357432
46	25.217275351405622	25.805566014056225	26.065198293172692	22.91196034136546
47	22.67860504518072	25.618489583333332	29.751819779116467	21.95108559236948
48	23.946567520080322	25.691437625502004	26.39934738955823	23.962647464859437
49	23.542215737951807	24.895284262048193	28.744273970883533	22.818226029116463
50	22.671937751004016	25.256494728915662	28.235990838353413	23.83557668172691
51	22.698214733935743	23.68262110943775	26.656234312248994	26.96292984437751
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	317.5
2	566.0
3	918.5
4	1271.0
5	939.0
6	607.0
7	620.0
8	633.0
9	598.5
10	564.0
11	569.0
12	574.0
13	560.0
14	546.0
15	553.5
16	561.0
17	546.5
18	532.0
19	576.0
20	620.0
21	688.0
22	756.0
23	830.0
24	904.0
25	986.5
26	1337.5
27	1606.0
28	1990.0
29	2374.0
30	2719.5
31	3065.0
32	3510.0
33	3955.0
34	4467.0
35	4979.0
36	5725.0
37	6471.0
38	7310.5
39	8150.0
40	9365.5
41	10581.0
42	11497.5
43	12414.0
44	14156.5
45	15899.0
46	21365.5
47	26832.0
48	24940.0
49	23048.0
50	22846.0
51	22644.0
52	21268.0
53	19892.0
54	18375.0
55	16858.0
56	15588.0
57	14318.0
58	13100.5
59	11883.0
60	11393.0
61	10903.0
62	10033.0
63	9163.0
64	8051.0
65	6939.0
66	5897.0
67	4855.0
68	4069.0
69	3283.0
70	2678.5
71	2074.0
72	1718.5
73	1363.0
74	1124.0
75	804.5
76	724.0
77	511.5
78	299.0
79	197.5
80	96.0
81	74.0
82	52.0
83	50.5
84	49.0
85	42.5
86	36.0
87	19.5
88	3.0
89	4.0
90	5.0
91	5.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.63020833333333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56584816775754	26.930377761044177
2	8.485846500187602	6.38648343373494
3	3.7499478884395714	4.233339608433735
4	2.407554091799725	3.6238704819277108
5	1.9948305332054863	3.753294427710843
6	1.6581898528369534	3.7438817771084336
7	1.42473006211698	3.7529022339357425
8	1.340309334222704	4.034889558232932
9	1.1474965606370118	3.8862481174698793
>10	6.13665735606787	33.43804907128514
>50	0.06253387251427857	1.5719126506024097
>100	0.01980239296285488	1.4973958333333335
>500	0.0031266936257139284	0.7416384287148594
>1k	0.0031266936257139284	2.4057166164658637
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	2123	0.8326273845381525	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	2075	0.8138020833333334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	1936	0.7592871485943775	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	840	0.3294427710843374	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	534	0.20943147590361447	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	517	0.20276418172690763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.1717808734939759	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	410	0.16079944779116467	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	338	0.13256149598393574	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTA	322	0.12628639558232932	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	288	0.11295180722891565	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.921937751004016E-4	0.0	0.0	0.12550200803212852	0.0
2	3.921937751004016E-4	0.0	0.0	0.7796812248995983	0.0
3	3.921937751004016E-4	0.0	0.0	0.9793078564257028	0.0
4	3.921937751004016E-4	0.0	0.0	1.4444496736947792	0.0
5	3.921937751004016E-4	0.0	0.0	3.0112638052208833	0.0
6	3.921937751004016E-4	0.0	0.0	3.7972201305220885	0.0
7	3.921937751004016E-4	0.0	0.0	4.500815763052209	0.0
8	3.921937751004016E-4	0.0	0.0	5.520911772088353	0.0
9	3.921937751004016E-4	0.0	0.0	5.929577685742972	0.0
10	3.921937751004016E-4	0.0	0.0	7.463055346385542	0.0
11	3.921937751004016E-4	0.0	0.0	8.345883534136545	0.0
12	3.921937751004016E-4	0.0	0.0	9.987606676706827	0.0
13	3.921937751004016E-4	0.0	0.0	10.34293423694779	0.0
14	3.921937751004016E-4	0.0	0.0	10.49706639056225	0.0
15	3.921937751004016E-4	0.0	0.0	10.838274974899598	0.0
16	3.921937751004016E-4	0.0	0.0	11.307338729919678	0.0
17	3.921937751004016E-4	0.0	0.0	11.852488077309237	0.0
18	3.921937751004016E-4	0.0	0.0	12.461957203815262	0.0
19	3.921937751004016E-4	0.0	0.0	12.915725401606426	0.0
20	3.921937751004016E-4	0.0	0.0	13.27183734939759	0.0
21	3.921937751004016E-4	0.0	0.0	13.666776480923694	0.0
22	3.921937751004016E-4	0.0	0.0	14.108778865461847	0.0
23	3.921937751004016E-4	0.0	0.0	14.516268197791165	0.0
24	3.921937751004016E-4	0.0	0.0	14.840612449799197	0.0
25	3.921937751004016E-4	0.0	0.0	15.157112826305221	0.0
26	3.921937751004016E-4	0.0	0.0	15.441453313253012	0.0
27	3.921937751004016E-4	0.0	0.0	15.75128639558233	0.0
28	3.921937751004016E-4	0.0	0.0	16.030136169678716	0.0
29	3.921937751004016E-4	0.0	0.0	16.332517570281123	0.0
30	3.921937751004016E-4	0.0	0.0	16.7980515813253	0.0
31	3.921937751004016E-4	0.0	0.0	17.135338227911646	0.0
32	3.921937751004016E-4	0.0	0.0	17.46085906124498	0.0
33	3.921937751004016E-4	0.0	0.0	17.76324046184739	0.0
34	3.921937751004016E-4	0.0	0.0	18.0667984437751	0.0
35	3.921937751004016E-4	0.0	0.0	18.404085090361445	0.0
36	3.921937751004016E-4	0.0	0.0	18.716663529116467	0.0
37	3.921937751004016E-4	0.0	0.0	19.034732680722893	0.0
38	3.921937751004016E-4	0.0	0.0	19.330054593373493	0.0
39	3.921937751004016E-4	0.0	0.0	19.64616277610442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAC	20	7.022951E-4	45.0	17
TTTACGG	20	7.022951E-4	45.0	2
TAACGCG	20	7.022951E-4	45.0	1
TTAAGCG	20	7.022951E-4	45.0	1
CGTAAGG	25	3.8822407E-5	45.0	2
TAGGACG	20	7.022951E-4	45.0	1
TACACGG	20	7.022951E-4	45.0	2
TGCACGG	20	7.022951E-4	45.0	2
CACAACG	170	0.0	43.676468	12
TACGGGA	100	0.0	42.75	4
TAGTAGG	65	0.0	41.53846	2
ACAACGA	180	0.0	41.25	13
ACGACCA	140	0.0	40.17857	28
AGCAACG	40	3.444129E-7	39.375	10
CGCGCAA	40	3.444129E-7	39.375	18
AATGGGC	80	0.0	39.375	4
AACGACG	40	3.444129E-7	39.375	13
CAACGAG	190	0.0	39.078945	14
ACGGGCG	75	0.0	39.0	5
AGGGATC	75	0.0	39.0	6
>>END_MODULE