SRR3550977_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532958
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.17318437850637386	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	891	0.16718015303269676	RNA PCR Primer, Index 40 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	661	0.1240247824406426	Illumina PCR Primer Index 8 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	636	0.11933398128933237	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAG	20	7.0298766E-4	45.000004	1
TCACCGA	20	7.0298766E-4	45.000004	28
TACGAAT	40	6.8030204E-9	45.000004	12
TCGGTAT	20	7.0298766E-4	45.000004	40
CGCCTTG	25	3.88798E-5	45.0	17
TCAACGG	35	1.2102828E-7	45.0	2
CACGACC	60	3.6379788E-12	41.249996	27
GGTACTC	45	1.9250365E-8	40.0	8
CGTAAGG	110	0.0	38.86364	2
TTAGGCG	35	6.241986E-6	38.571426	1
ACACGAC	65	9.094947E-12	38.076927	26
GCGTAAG	65	9.094947E-12	38.076927	1
CGTTTTT	770	0.0	37.694805	1
TCGATCA	30	1.1391006E-4	37.499996	17
ATCGTCC	30	1.1391006E-4	37.499996	19
GCGATCA	60	1.546141E-10	37.499996	9
GTAAGCG	30	1.1391006E-4	37.499996	1
GCGCTAG	30	1.1391006E-4	37.499996	1
TGCGTAG	30	1.1391006E-4	37.499996	1
TAAGCGG	115	0.0	37.173912	2