##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	532958
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00591416209157	33.0	31.0	34.0	30.0	34.0
2	32.03954345370555	33.0	31.0	34.0	30.0	34.0
3	31.93637584950409	33.0	31.0	34.0	30.0	34.0
4	35.651349637307256	37.0	35.0	37.0	33.0	37.0
5	33.39305536271151	37.0	35.0	37.0	30.0	37.0
6	34.48009223991384	37.0	35.0	37.0	30.0	37.0
7	35.67147505056683	37.0	35.0	37.0	32.0	37.0
8	35.596354684609295	37.0	35.0	37.0	33.0	37.0
9	37.52502823862293	39.0	37.0	39.0	35.0	39.0
10	37.000986944562236	39.0	37.0	39.0	33.0	39.0
11	37.0493322175481	39.0	37.0	39.0	34.0	39.0
12	37.10259345014054	39.0	37.0	39.0	34.0	39.0
13	37.21463980276119	39.0	37.0	39.0	34.0	39.0
14	38.39465211142341	40.0	38.0	41.0	34.0	41.0
15	38.520288653139644	40.0	38.0	41.0	34.0	41.0
16	38.30099932827728	40.0	38.0	41.0	34.0	41.0
17	38.28055306421894	40.0	37.0	41.0	34.0	41.0
18	38.159592313090336	40.0	37.0	41.0	34.0	41.0
19	38.008107580709925	40.0	37.0	41.0	34.0	41.0
20	38.283358163307426	40.0	37.0	41.0	34.0	41.0
21	38.223338424416184	40.0	37.0	41.0	34.0	41.0
22	38.3645184048274	40.0	37.0	41.0	34.0	41.0
23	38.43443010518652	40.0	38.0	41.0	34.0	41.0
24	38.35725892096563	40.0	37.0	41.0	34.0	41.0
25	37.93659350267751	40.0	37.0	41.0	33.0	41.0
26	38.12075623219841	40.0	37.0	41.0	34.0	41.0
27	38.17453720555841	40.0	37.0	41.0	34.0	41.0
28	37.941132321871514	40.0	37.0	41.0	33.0	41.0
29	37.91794662994082	40.0	37.0	41.0	33.0	41.0
30	37.74717519954668	40.0	36.0	41.0	33.0	41.0
31	37.6995579388995	40.0	36.0	41.0	33.0	41.0
32	37.704794749304824	40.0	36.0	41.0	33.0	41.0
33	37.639076625174965	40.0	36.0	41.0	33.0	41.0
34	37.58265754524747	40.0	36.0	41.0	33.0	41.0
35	37.56363728473914	40.0	36.0	41.0	33.0	41.0
36	37.46750963490557	40.0	36.0	41.0	33.0	41.0
37	37.44286416565658	40.0	36.0	41.0	33.0	41.0
38	37.315176805677	40.0	36.0	41.0	32.0	41.0
39	37.24344882711208	40.0	36.0	41.0	32.0	41.0
40	37.097011396770476	40.0	35.0	41.0	32.0	41.0
41	36.97806581381647	40.0	35.0	41.0	31.0	41.0
42	36.99290938497968	40.0	35.0	41.0	31.0	41.0
43	36.95327586789203	40.0	35.0	41.0	31.0	41.0
44	36.890359090209735	39.0	35.0	41.0	31.0	41.0
45	36.76616168628672	39.0	35.0	41.0	31.0	41.0
46	36.70664667759936	39.0	35.0	41.0	31.0	41.0
47	36.672687153584334	39.0	35.0	41.0	31.0	41.0
48	36.53746636695575	39.0	35.0	41.0	31.0	41.0
49	36.45955966511433	39.0	35.0	41.0	30.0	41.0
50	36.395378247441634	39.0	35.0	41.0	30.0	41.0
51	35.12777179440031	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	29.0
10	31.0
11	18.0
12	15.0
13	14.0
14	14.0
15	15.0
16	35.0
17	57.0
18	117.0
19	222.0
20	355.0
21	628.0
22	966.0
23	1522.0
24	2205.0
25	3078.0
26	4190.0
27	4600.0
28	5323.0
29	6361.0
30	7589.0
31	9355.0
32	12563.0
33	17459.0
34	29715.0
35	37926.0
36	39592.0
37	63118.0
38	109266.0
39	176506.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72982111160729	19.03902371293798	23.084182993781873	15.146972181672853
2	31.099824001140803	24.278273334859406	26.543367394804097	18.078535269195697
3	32.8357956912177	23.749526229083717	26.506403881731767	16.90827419796682
4	28.61763966391348	26.09792891747567	26.237339527692615	19.04709189091823
5	23.423046468952524	35.136915104004444	23.358501045110497	18.081537381932534
6	25.06276291940453	35.8538571519707	24.755421627970684	14.327958300654084
7	81.85035218535045	5.601754734894683	8.221098097786317	4.32679498196856
8	83.25534094619088	4.899447986520514	7.284814187984795	4.56039687930381
9	77.50047846171744	7.117258770859992	9.830042892685729	5.552219874736846
10	38.02175781206024	26.730248912672295	18.910308129346028	16.337685145921444
11	28.77787743124224	28.456463736354458	24.049925134813623	18.71573369758968
12	24.93104522307574	24.40079706093163	30.27499352669441	20.39316418929822
13	24.563474044859067	25.52583880906188	30.88160793158185	19.0290792144972
14	21.49381377144165	28.979018984610423	29.633667193287277	19.893500050660652
15	20.931668161468632	26.787289054672225	33.34577959238814	18.935263191470998
16	23.27312846415665	27.356564682395234	29.52896851159003	19.841338341858084
17	24.051238559135992	25.36597630582522	27.18094108729018	23.4018440477486
18	26.236401367462353	25.45116125473302	28.08626570949306	20.226171668311572
19	24.950371323819137	28.103527857729876	26.616356260718483	20.329744557732504
20	24.214103175109482	26.972294252079905	27.354125465796553	21.45947710701406
21	24.743976073161488	28.38703987931507	27.559207292131838	19.309776755391606
22	22.840636598005847	26.43716765673843	27.531625381362133	23.19057036389359
23	21.441839694685136	27.365946284697856	27.57665707241471	23.615556948202297
24	21.374667422198375	26.406208369139783	28.53808367638726	23.68104053227459
25	21.426453866908837	28.419500223282135	27.024080696790364	23.12996521301866
26	20.222981923528682	28.073881994453597	28.254196390709964	23.448939691307757
27	21.717283538290072	27.23197700381644	27.743649593401354	23.307089864492138
28	20.589052045376935	27.520930354737143	29.76144461664897	22.128572983236953
29	22.565005122354854	26.754078182520953	28.505248068328086	22.175668626796107
30	21.809786136993907	28.24631584477576	28.213855500808695	21.730042517421637
31	22.501773122835196	29.231008822458804	26.211446305337383	22.05577174936862
32	22.72242840899283	27.85360197238807	27.933908488098496	21.490061130520605
33	22.85752348215056	28.058308534631248	25.795090795147086	23.289077188071104
34	20.56297119097565	30.017562359510507	27.434431981506986	21.98503446800686
35	20.97876380502779	28.324746040025666	27.601049238401526	23.09544091654502
36	21.00690861193565	30.11231654276697	26.232085830403147	22.648689014894234
37	21.275597701882702	29.220313795833817	26.29944573493596	23.204642767347522
38	21.813914042007063	29.304372952465297	25.74161566202215	23.14009734350549
39	22.938017629907048	27.527309844302927	26.21182156942949	23.32285095636054
40	21.77244735982948	27.109265645698162	27.638763279658058	23.479523714814302
41	20.358076996686417	27.206834309645412	27.28920477786242	25.14588391580575
42	21.19454065798806	27.430116444447776	27.03158597863246	24.343756918931696
43	21.02867392927773	26.169604359067694	27.606490567737048	25.195231143917535
44	21.041432908409295	27.960364606591888	26.743383155895962	24.254819329102855
45	21.36978898900101	27.715504786493494	26.937582323560207	23.97712390094529
46	21.541847575231067	27.8369027202894	26.758768983672258	23.86248072080727
47	20.450954859482362	27.258620754355878	28.055118789848354	24.235305596313406
48	20.70557154597548	26.430788167172647	28.764180291880407	24.09945999497146
49	22.237211937901296	26.33547108777802	27.727888501532956	23.699428472787723
50	20.419620307791607	26.63117919235662	28.588556696775356	24.360643803076414
51	20.381343370396916	26.385756476120072	27.288078985586107	25.944821167896908
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103.0
1	380.0
2	657.0
3	1473.0
4	2289.0
5	1648.5
6	1008.0
7	1002.5
8	997.0
9	978.0
10	959.0
11	1012.0
12	1065.0
13	1071.0
14	1077.0
15	1034.0
16	991.0
17	1084.0
18	1177.0
19	1185.5
20	1194.0
21	1387.0
22	1580.0
23	1895.5
24	2211.0
25	2606.0
26	3703.5
27	4406.0
28	5302.0
29	6198.0
30	7252.5
31	8307.0
32	9648.0
33	10989.0
34	12796.0
35	14603.0
36	16623.0
37	18643.0
38	20105.5
39	21568.0
40	24117.0
41	26666.0
42	29720.0
43	32774.0
44	36446.0
45	40118.0
46	44423.5
47	48729.0
48	51327.5
49	53926.0
50	52604.5
51	51283.0
52	46459.5
53	41636.0
54	36598.0
55	31560.0
56	28860.5
57	26161.0
58	23512.0
59	20863.0
60	18998.0
61	17133.0
62	14889.5
63	12646.0
64	11064.0
65	9482.0
66	7810.5
67	6139.0
68	5211.5
69	4284.0
70	3318.5
71	2353.0
72	2018.5
73	1684.0
74	1448.0
75	947.0
76	682.0
77	479.5
78	277.0
79	231.0
80	185.0
81	136.0
82	87.0
83	62.0
84	37.0
85	21.5
86	6.0
87	5.0
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	532958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.39798886852762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41924072436564	28.925704287943066
2	9.31551355010867	7.34024998303599
3	3.630424523463127	4.290942748902898
4	2.047602054197121	3.2268561175372983
5	1.4696157850941485	2.8949953171075866
6	1.178338524735228	2.785450084852546
7	0.993621379359777	2.7402678830483285
8	0.9236437959000736	2.9111766391484553
9	0.7934281816035479	2.813352720011342
>10	6.159501959034059	39.01631616992992
>50	0.05001586085914588	1.3500906690965713
>100	0.017148295151523937	1.1207591709449904
>500	0.001905366127947104	0.5838382084410357
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	923	0.17318437850637386	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	891	0.16718015303269676	RNA PCR Primer, Index 40 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	661	0.1240247824406426	Illumina PCR Primer Index 8 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	636	0.11933398128933237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.628961381572282E-4	0.0	0.0	0.10263472919066793	0.0
2	5.628961381572282E-4	0.0	0.0	0.5006022988678283	0.0
3	5.628961381572282E-4	0.0	0.0	0.7293257630057153	0.0
4	5.628961381572282E-4	0.0	0.0	1.0933319323473896	0.0
5	5.628961381572282E-4	0.0	0.0	1.9519361750832147	0.0
6	5.628961381572282E-4	0.0	0.0	2.8675805598189728	0.0
7	5.628961381572282E-4	0.0	0.0	3.5357382758116023	0.0
8	5.628961381572282E-4	0.0	0.0	4.708438563639161	0.0
9	5.628961381572282E-4	0.0	0.0	5.182772376059652	0.0
10	5.628961381572282E-4	0.0	0.0	6.176096427861108	0.0
11	5.628961381572282E-4	0.0	0.0	7.2037571440901536	0.0
12	5.628961381572282E-4	0.0	0.0	8.189575914049513	0.0
13	5.628961381572282E-4	0.0	0.0	8.635952551608193	0.0
14	5.628961381572282E-4	0.0	0.0	8.827337238581652	0.0
15	5.628961381572282E-4	0.0	0.0	9.105783194923427	0.0
16	5.628961381572282E-4	0.0	0.0	9.651980080981991	0.0
17	5.628961381572282E-4	0.0	0.0	10.303438544875956	0.0
18	7.505281842096375E-4	0.0	0.0	11.10819239039474	0.0
19	7.505281842096375E-4	0.0	0.0	11.537869775854757	0.0
20	7.505281842096375E-4	0.0	0.0	12.007325155077886	0.0
21	7.505281842096375E-4	0.0	0.0	12.544328070879882	0.0
22	7.505281842096375E-4	0.0	0.0	13.089211532616078	0.0
23	7.505281842096375E-4	0.0	0.0	13.643664228700949	0.0
24	7.505281842096375E-4	0.0	0.0	14.109742231095133	0.0
25	7.505281842096375E-4	0.0	0.0	14.519530619673596	0.0
26	7.505281842096375E-4	0.0	0.0	14.910931067738922	0.0
27	7.505281842096375E-4	0.0	0.0	15.323158672916065	0.0
28	7.505281842096375E-4	0.0	0.0	15.758840283849759	0.0
29	7.505281842096375E-4	0.0	0.0	16.207093241868964	0.0
30	7.505281842096375E-4	0.0	0.0	16.785375207802492	0.0
31	9.381602302620469E-4	0.0	0.0	17.268340094341394	0.0
32	9.381602302620469E-4	0.0	0.0	17.786579805538146	0.0
33	9.381602302620469E-4	0.0	0.0	18.392256050195325	0.0
34	9.381602302620469E-4	0.0	0.0	18.863587749878977	0.0
35	9.381602302620469E-4	0.0	0.0	19.341486571174464	0.0
36	0.0013134243223668657	0.0	0.0	19.784110567812096	0.0
37	0.0013134243223668657	0.0	0.0	20.22579640421947	0.0
38	0.0013134243223668657	0.0	0.0	20.745161907692538	0.0
39	0.0013134243223668657	0.0	0.0	21.219120456020924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAG	20	7.0298766E-4	45.000004	1
TCACCGA	20	7.0298766E-4	45.000004	28
TACGAAT	40	6.8030204E-9	45.000004	12
TCGGTAT	20	7.0298766E-4	45.000004	40
CGCCTTG	25	3.88798E-5	45.0	17
TCAACGG	35	1.2102828E-7	45.0	2
CACGACC	60	3.6379788E-12	41.249996	27
GGTACTC	45	1.9250365E-8	40.0	8
CGTAAGG	110	0.0	38.86364	2
TTAGGCG	35	6.241986E-6	38.571426	1
ACACGAC	65	9.094947E-12	38.076927	26
GCGTAAG	65	9.094947E-12	38.076927	1
CGTTTTT	770	0.0	37.694805	1
TCGATCA	30	1.1391006E-4	37.499996	17
ATCGTCC	30	1.1391006E-4	37.499996	19
GCGATCA	60	1.546141E-10	37.499996	9
GTAAGCG	30	1.1391006E-4	37.499996	1
GCGCTAG	30	1.1391006E-4	37.499996	1
TGCGTAG	30	1.1391006E-4	37.499996	1
TAAGCGG	115	0.0	37.173912	2
>>END_MODULE