FastQCFastQC Report
Sat 18 Jun 2016
SRR3550974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371065
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC124553.356554781507283No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC115523.1132011911659685No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG110652.9819573390106857No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC38011.0243488337622788No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT28890.7785697923544392No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT26220.7066147440475388No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT20320.5476129519086953No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA20000.5389891258943851No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC19000.5120396695996658No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.3759449153113336No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC10610.2859337312869713No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT8170.2201770579278563No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGT5810.15657634107231888No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT5630.1517254389392694No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGTGTAT5090.137172732540121No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATG4980.1342082923477019No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT4860.13097435759233558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC302.1615142E-645.0000049
TCTATGC302.1615142E-645.00000430
CGACGTC207.027106E-445.045
GCGAACC253.885683E-545.033
TCGATTA207.027106E-445.041
TACGGGA453.8380676E-1045.04
CGACAGG253.885683E-545.02
CCGTATT253.885683E-545.041
CACTAGA207.027106E-445.020
CGAATGC551.8189894E-1245.045
ACTCGTT207.027106E-445.012
CGGTGAT253.885683E-545.031
TGCTCGG207.027106E-445.02
CTATTAG207.027106E-445.01
ATTGCGG351.2089549E-745.02
TATCGGG207.027106E-445.03
TACGGCT13700.043.0291987
CCGATGA7000.042.7518
CGATGAA7000.042.7519
ACGGCTG13850.042.725638