##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371065
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229951625725953	31.0	31.0	33.0	28.0	34.0
2	31.14723296457494	31.0	31.0	34.0	28.0	34.0
3	31.09432579197715	31.0	31.0	34.0	28.0	34.0
4	35.022435422365355	35.0	35.0	37.0	32.0	37.0
5	35.21436945009634	35.0	35.0	37.0	33.0	37.0
6	35.12360907118699	36.0	35.0	37.0	33.0	37.0
7	35.433519733739374	37.0	35.0	37.0	33.0	37.0
8	34.82547801598103	37.0	35.0	37.0	32.0	37.0
9	36.62175090617547	39.0	35.0	39.0	32.0	39.0
10	36.300041771657256	37.0	35.0	39.0	32.0	39.0
11	36.544756309541455	38.0	35.0	39.0	32.0	39.0
12	36.67205476129519	39.0	35.0	39.0	32.0	39.0
13	36.59608963389164	39.0	35.0	39.0	32.0	39.0
14	37.651249781035666	39.0	36.0	41.0	32.0	41.0
15	37.598436931534906	39.0	36.0	41.0	32.0	41.0
16	37.8552032662741	39.0	37.0	41.0	33.0	41.0
17	37.53502755581906	39.0	36.0	41.0	32.0	41.0
18	37.50444531281581	39.0	36.0	41.0	32.0	41.0
19	37.42398232115667	39.0	36.0	40.0	32.0	41.0
20	37.22382870925579	39.0	35.0	41.0	31.0	41.0
21	37.43357632759759	39.0	36.0	40.0	32.0	41.0
22	37.53337824909382	39.0	36.0	41.0	32.0	41.0
23	37.56742349723094	39.0	36.0	41.0	33.0	41.0
24	37.4185627854958	39.0	35.0	41.0	32.0	41.0
25	37.34682333283926	39.0	35.0	41.0	32.0	41.0
26	37.29511810599221	39.0	35.0	41.0	32.0	41.0
27	37.306479996766065	39.0	36.0	41.0	32.0	41.0
28	37.15252044789997	39.0	35.0	41.0	31.0	41.0
29	37.160074380499374	39.0	35.0	41.0	31.0	41.0
30	36.9445865279668	39.0	35.0	41.0	31.0	41.0
31	36.91672348510369	39.0	35.0	41.0	31.0	41.0
32	36.652432862167004	39.0	35.0	41.0	30.0	41.0
33	36.689986929513694	39.0	35.0	41.0	30.0	41.0
34	36.50706210502203	39.0	35.0	41.0	30.0	41.0
35	36.43556250252651	39.0	35.0	41.0	30.0	41.0
36	36.368172692115934	39.0	35.0	41.0	30.0	41.0
37	36.41045638904235	39.0	35.0	41.0	30.0	41.0
38	36.14598520474849	39.0	35.0	41.0	30.0	41.0
39	36.04216242437309	39.0	35.0	40.0	30.0	41.0
40	36.000385377225015	39.0	35.0	40.0	30.0	41.0
41	35.76060797973401	38.0	35.0	40.0	27.0	41.0
42	35.92493498443669	39.0	35.0	40.0	29.0	41.0
43	35.999857167881636	39.0	35.0	40.0	30.0	41.0
44	36.12792098419414	39.0	35.0	40.0	30.0	41.0
45	36.041461738509426	39.0	35.0	40.0	29.0	41.0
46	35.923733038685945	39.0	35.0	40.0	29.0	41.0
47	35.82690903211028	38.0	35.0	40.0	29.0	41.0
48	35.769183296726986	38.0	35.0	40.0	28.0	41.0
49	35.80498025952326	38.0	35.0	40.0	28.0	41.0
50	35.62778219449422	38.0	35.0	40.0	28.0	41.0
51	34.5180682629728	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	8.0
11	4.0
12	4.0
13	8.0
14	5.0
15	16.0
16	23.0
17	53.0
18	107.0
19	276.0
20	685.0
21	1317.0
22	1868.0
23	2204.0
24	2591.0
25	3175.0
26	3804.0
27	4258.0
28	4666.0
29	5447.0
30	6788.0
31	8864.0
32	11983.0
33	15960.0
34	25347.0
35	28772.0
36	31464.0
37	46745.0
38	81034.0
39	83566.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.72894236858772	17.366229636317087	19.877649468421975	11.027178526673225
2	28.285610337811434	36.076159163488875	20.281621818279817	15.356608680419873
3	27.63046905528681	36.09475428833223	22.672038591621412	13.602738064759542
4	26.011884710225967	20.30129492137496	37.45677980946734	16.230040558931723
5	34.751323892040475	24.34155740907927	21.326182744263132	19.580935954617114
6	22.476385538921754	41.04698637704984	23.455459286108905	13.021168797919502
7	67.35477611739184	5.242208238448789	22.294746203495343	5.108269440664034
8	64.24211391535177	16.741541239405493	12.361176613261827	6.65516823198092
9	59.05596054599599	7.208171075148559	12.666513953080996	21.06935442577446
10	41.25853960896339	20.940266530122756	21.470092840876937	16.331101020036918
11	32.89720129896379	21.404875156643715	25.34111274305041	20.356810801342082
12	25.594707126783717	18.708582054357052	29.882096128710607	25.814614690148623
13	24.658213520542223	20.68236023338229	36.669316696535645	17.990109549539838
14	20.68828911376713	30.692196784929866	26.415318070957916	22.204196030345088
15	16.049209707194155	22.772560063600718	39.22601161521566	21.952218613989462
16	18.00520124506488	24.629916591432767	27.777882581218922	29.58699958228343
17	18.876477167073155	25.66450621858704	31.579103391589076	23.87991322275073
18	18.235888590947678	24.709956476628083	30.829908506595878	26.22424642582836
19	19.255925511702802	26.201878377103743	26.986377049843018	27.55581906135044
20	23.686685621117594	24.873270181774082	33.1650788945333	18.27496530257502
21	21.470901324565776	31.512538234541122	27.888105857464325	19.12845458342878
22	19.042216323285675	22.02255669491868	30.896473663643835	28.038753318151805
23	21.12379232748979	29.268726503442792	28.9369786964548	20.670502472612615
24	23.253607858461457	23.86077910878148	26.36788702787921	26.51772600487785
25	18.23400212900705	33.897565116623774	24.846320725479362	23.02211202888982
26	18.50807809952434	23.16117122337057	30.455580558662227	27.87517011844286
27	24.302750192014877	25.925915944645816	25.015832805573147	24.75550105776616
28	17.962621104119226	25.142225755595383	31.86368965006131	25.031463490224088
29	21.64903723067387	21.171762359694394	28.23790980016978	28.941290609461955
30	21.032164176087747	26.480535755191138	27.30896204169081	25.178338027030307
31	26.59183700968833	22.23437942139517	24.044574400711465	27.129209168205033
32	25.005861506744104	27.7657553258863	26.4123536307655	20.8160295366041
33	25.76826162532171	21.09899882769865	25.082936951746998	28.04980259523264
34	23.115357147669545	22.22333014431434	29.397814399094496	25.263498308921616
35	20.344414051446513	24.591109374368372	26.194063034778274	28.87041353940684
36	23.821702397154137	22.710576314122864	28.008030937975825	25.45969035074717
37	18.76733186907954	22.8941021114899	34.022610593831274	24.315955425599288
38	27.163434977699325	23.85215528276717	24.4827725600636	24.501637179469903
39	22.631344912616388	20.90604072062846	34.31905461307318	22.14355975368197
40	23.9214153854446	23.816312505895194	29.878862193955236	22.38340991470497
41	19.057577513373666	24.74957217738132	31.833775753574173	24.35907455567084
42	19.72080363278671	25.505773921011144	32.06608006683465	22.707342379367496
43	23.784242652904478	20.785307156428118	29.865656960370824	25.56479323029658
44	22.09181679759611	20.586959158098985	30.48603344427526	26.835190600029645
45	21.280638163125058	19.938016250522146	28.02689555738213	30.754450028970666
46	27.130556641019766	23.94243596135448	26.959427593548302	21.967579804077452
47	18.841442873890017	21.515367927452065	37.271367550159674	22.37182164849824
48	21.486262514653767	24.083381617775864	27.891070297656746	26.53928556991363
49	19.997574548933475	20.219638068801963	35.87996712166332	23.902820260601242
50	22.45078355544177	19.13680891488014	32.10650425127673	26.30590327840136
51	20.163852694271895	19.667713203886112	27.91721127026262	32.251222831579376
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	237.5
2	374.0
3	1466.0
4	2558.0
5	1800.5
6	1043.0
7	986.5
8	930.0
9	923.5
10	917.0
11	920.5
12	924.0
13	908.0
14	892.0
15	863.0
16	834.0
17	785.0
18	736.0
19	738.5
20	741.0
21	847.5
22	954.0
23	917.5
24	881.0
25	1070.5
26	1572.5
27	1885.0
28	2553.5
29	3222.0
30	3659.5
31	4097.0
32	4623.5
33	5150.0
34	5977.5
35	6805.0
36	7212.0
37	7619.0
38	8719.0
39	9819.0
40	11589.5
41	13360.0
42	14822.5
43	16285.0
44	18183.0
45	20081.0
46	46129.0
47	72177.0
48	55338.0
49	38499.0
50	38311.0
51	38123.0
52	32025.0
53	25927.0
54	22916.0
55	19905.0
56	18417.5
57	16930.0
58	15763.5
59	14597.0
60	13944.5
61	13292.0
62	11819.5
63	10347.0
64	8732.0
65	7117.0
66	5780.0
67	4443.0
68	3862.0
69	3281.0
70	2594.5
71	1908.0
72	1624.5
73	1341.0
74	1096.0
75	662.0
76	473.0
77	343.0
78	213.0
79	160.5
80	108.0
81	75.5
82	43.0
83	28.0
84	13.0
85	9.0
86	5.0
87	2.5
88	0.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	371065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.2586655449841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.82914855148854	24.554589591449048
2	9.995120312237194	6.6484872709314775
3	3.61039338020689	3.6023059775437667
4	1.8596159275939268	2.4739337671188713
5	1.2373657671829368	2.0576567103774974
6	0.9971628200259014	1.9898582835080776
7	0.7998663100560037	1.8621740260797226
8	0.7406159990106242	1.9705519846686903
9	0.6700355964820672	2.0056040825967894
>10	6.143865650776737	34.30559047246801
>50	0.07138369635172025	1.6046936723725467
>100	0.03325831307296057	2.0209751680804886
>500	0.003244713470532739	0.6663741376530246
>1k	0.006489426941065478	4.775231674679569
>5k	0.0	0.0
>10k+	0.002433535102899554	9.46197318047242
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	12455	3.356554781507283	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	11552	3.1132011911659685	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	11065	2.9819573390106857	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3801	1.0243488337622788	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT	2889	0.7785697923544392	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCT	2622	0.7066147440475388	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCT	2032	0.5476129519086953	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGTGTATTCGTA	2000	0.5389891258943851	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTC	1900	0.5120396695996658	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1395	0.3759449153113336	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1061	0.2859337312869713	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	817	0.2201770579278563	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGT	581	0.15657634107231888	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTT	563	0.1517254389392694	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGTGTAT	509	0.137172732540121	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATG	498	0.1342082923477019	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTAT	486	0.13097435759233558	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6949456294719256E-4	0.0	0.0	0.22960936763100803	0.0
2	2.6949456294719256E-4	0.0	0.0	1.8064220554350316	0.0
3	2.6949456294719256E-4	0.0	0.0	2.1758991012356326	0.0
4	2.6949456294719256E-4	0.0	0.0	3.189468152480024	0.0
5	2.6949456294719256E-4	0.0	0.0	7.68032554943204	0.0
6	2.6949456294719256E-4	0.0	0.0	8.547559052996105	0.0
7	2.6949456294719256E-4	0.0	0.0	10.026275719887352	0.0
8	2.6949456294719256E-4	0.0	0.0	11.660221255036179	0.0
9	2.6949456294719256E-4	0.0	0.0	12.072278441782437	0.0
10	2.6949456294719256E-4	0.0	0.0	15.991537870723459	0.0
11	2.6949456294719256E-4	0.0	0.0	17.350868446229097	0.0
12	2.6949456294719256E-4	0.0	0.0	21.9198792664358	0.0
13	2.6949456294719256E-4	0.0	0.0	22.522199614622775	0.0
14	2.6949456294719256E-4	0.0	0.0	22.84909651947772	0.0
15	2.6949456294719256E-4	0.0	0.0	23.78020023446027	0.0
16	2.6949456294719256E-4	0.0	0.0	24.659830487919905	0.0
17	2.6949456294719256E-4	0.0	0.0	25.494185654804415	0.0
18	2.6949456294719256E-4	0.0	0.0	26.342824033525122	0.0
19	2.6949456294719256E-4	0.0	0.0	27.73772789133979	0.0
20	2.6949456294719256E-4	0.0	0.0	28.383436864161265	0.0
21	2.6949456294719256E-4	0.0	0.0	29.019983021842535	0.0
22	2.6949456294719256E-4	0.0	0.0	29.831970140002426	0.0
23	2.6949456294719256E-4	0.0	0.0	30.489806368156522	0.0
24	5.389891258943851E-4	0.0	0.0	31.0150512713406	0.0
25	5.389891258943851E-4	0.0	0.0	31.478042930483877	0.0
26	5.389891258943851E-4	0.0	0.0	31.934566720116422	0.0
27	5.389891258943851E-4	0.0	0.0	32.476789780766175	0.0
28	5.389891258943851E-4	0.0	0.0	32.93385255952461	0.0
29	5.389891258943851E-4	0.0	0.0	33.40977995768935	0.0
30	5.389891258943851E-4	0.0	0.0	33.91912468165955	0.0
31	5.389891258943851E-4	0.0	0.0	34.412838720978804	0.0
32	5.389891258943851E-4	0.0	0.0	34.87124897255198	0.0
33	5.389891258943851E-4	0.0	0.0	35.33585759907294	0.0
34	5.389891258943851E-4	0.0	0.0	35.78321857356528	0.0
35	5.389891258943851E-4	0.0	0.0	36.264805357551914	0.0
36	5.389891258943851E-4	0.0	0.0	36.711896837481305	0.0
37	5.389891258943851E-4	0.0	0.0	37.17434950749869	0.0
38	5.389891258943851E-4	0.0	0.0	37.60985272122135	0.0
39	5.389891258943851E-4	0.0	0.0	38.06287308153558	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTC	30	2.1615142E-6	45.000004	9
TCTATGC	30	2.1615142E-6	45.000004	30
CGACGTC	20	7.027106E-4	45.0	45
GCGAACC	25	3.885683E-5	45.0	33
TCGATTA	20	7.027106E-4	45.0	41
TACGGGA	45	3.8380676E-10	45.0	4
CGACAGG	25	3.885683E-5	45.0	2
CCGTATT	25	3.885683E-5	45.0	41
CACTAGA	20	7.027106E-4	45.0	20
CGAATGC	55	1.8189894E-12	45.0	45
ACTCGTT	20	7.027106E-4	45.0	12
CGGTGAT	25	3.885683E-5	45.0	31
TGCTCGG	20	7.027106E-4	45.0	2
CTATTAG	20	7.027106E-4	45.0	1
ATTGCGG	35	1.2089549E-7	45.0	2
TATCGGG	20	7.027106E-4	45.0	3
TACGGCT	1370	0.0	43.029198	7
CCGATGA	700	0.0	42.75	18
CGATGAA	700	0.0	42.75	19
ACGGCTG	1385	0.0	42.72563	8
>>END_MODULE