SRR3550973_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	9815	2.939256366641911	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	9017	2.700282695670923	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	8905	2.6667425313241178	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3024	0.9055844373637431	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	2183	0.6537337390096069	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1985	0.5944395198965047	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	1493	0.4471023693730385	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1470	0.4402146570518195	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	1445	0.4327280132244077	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1096	0.3282144653937376	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.30755132843008076	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	743	0.22250305455068156	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	472	0.14134783546153662	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG	444	0.1329627943748353	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG	409	0.12248149301645865	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT	358	0.10720873960853837	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	357	0.1069092738554419	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	348	0.1042140820775736	Illumina Single End Adapter 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	45	3.8380676E-10	45.000004	41
GACGAAT	30	2.1608594E-6	45.000004	20
GTACGAG	30	2.1608594E-6	45.000004	1
AAGTACG	20	7.0260896E-4	45.0	1
CGAACCC	35	1.2084638E-7	45.0	34
GCGAACC	35	1.2084638E-7	45.0	33
TCTAGCG	20	7.0260896E-4	45.0	1
CCGTAAG	20	7.0260896E-4	45.0	42
TGTTCGG	25	3.884842E-5	45.0	2
TCAGACG	20	7.0260896E-4	45.0	22
CGTAAGG	40	6.7902874E-9	45.0	2
CGTAAGC	25	3.884842E-5	45.0	43
CGCACGG	25	3.884842E-5	45.0	2
GCTACGA	40	6.7902874E-9	45.0	10
GTAAGCG	25	3.884842E-5	45.0	1
ATACAGG	20	7.0260896E-4	45.0	2
GGCTACG	20	7.0260896E-4	45.0	1
TGCAACG	20	7.0260896E-4	45.0	1
CCGATGA	555	0.0	43.783783	18
TACGGCT	1075	0.0	42.488373	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content