##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	333928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.347577920988957	31.0	31.0	33.0	30.0	34.0
2	31.268629764500133	31.0	31.0	34.0	28.0	34.0
3	31.226111616875492	31.0	31.0	34.0	28.0	34.0
4	35.118124266308904	35.0	35.0	37.0	32.0	37.0
5	35.32296483074196	35.0	35.0	37.0	33.0	37.0
6	35.237524256726	37.0	35.0	37.0	33.0	37.0
7	35.55025933734218	37.0	35.0	37.0	33.0	37.0
8	35.059518818427925	37.0	35.0	37.0	32.0	37.0
9	36.87187058288014	39.0	37.0	39.0	32.0	39.0
10	36.48567954768693	38.0	35.0	39.0	32.0	39.0
11	36.68198533815673	39.0	35.0	39.0	32.0	39.0
12	36.741045973982416	39.0	35.0	39.0	33.0	39.0
13	36.69108610239333	39.0	35.0	39.0	32.0	39.0
14	37.70510409429578	39.0	36.0	41.0	33.0	41.0
15	37.70921276442826	40.0	36.0	41.0	32.0	41.0
16	37.89046740614743	39.0	36.0	41.0	33.0	41.0
17	37.58969897702499	39.0	36.0	41.0	32.0	41.0
18	37.58811779784864	39.0	36.0	41.0	33.0	41.0
19	37.488982654943584	39.0	36.0	41.0	32.0	41.0
20	37.32893018854364	39.0	35.0	41.0	32.0	41.0
21	37.51223317601399	39.0	36.0	41.0	33.0	41.0
22	37.625895402601756	39.0	36.0	41.0	33.0	41.0
23	37.6693329100884	39.0	36.0	41.0	33.0	41.0
24	37.55022040679428	39.0	35.0	41.0	33.0	41.0
25	37.453436070051026	39.0	35.0	41.0	33.0	41.0
26	37.408111928319876	39.0	35.0	41.0	33.0	41.0
27	37.43082041637718	39.0	35.0	41.0	33.0	41.0
28	37.29597997173043	39.0	35.0	41.0	32.0	41.0
29	37.368133250281495	39.0	35.0	41.0	32.0	41.0
30	37.103528305502984	39.0	35.0	41.0	32.0	41.0
31	37.13828130614983	39.0	35.0	41.0	32.0	41.0
32	36.95363072279054	39.0	35.0	41.0	31.0	41.0
33	36.92571751994442	39.0	35.0	41.0	31.0	41.0
34	36.73274178864905	39.0	35.0	41.0	31.0	41.0
35	36.774690951342805	39.0	35.0	41.0	31.0	41.0
36	36.66299621475288	39.0	35.0	41.0	31.0	41.0
37	36.59240614743298	39.0	35.0	41.0	31.0	41.0
38	36.51029563259146	39.0	35.0	41.0	31.0	41.0
39	36.46310581921851	39.0	35.0	41.0	31.0	41.0
40	36.37768321314775	39.0	35.0	41.0	30.0	41.0
41	36.14867576243981	39.0	35.0	40.0	30.0	41.0
42	36.19153829568051	39.0	35.0	40.0	30.0	41.0
43	36.18842085719077	39.0	35.0	40.0	30.0	41.0
44	36.19013380129849	39.0	35.0	40.0	30.0	41.0
45	36.2145941640114	39.0	35.0	40.0	30.0	41.0
46	36.16224755037014	39.0	35.0	40.0	30.0	41.0
47	36.03313888023766	38.0	35.0	40.0	30.0	41.0
48	35.972817493591435	38.0	35.0	40.0	30.0	41.0
49	36.05104094295776	38.0	35.0	40.0	30.0	41.0
50	35.90064624709518	38.0	35.0	40.0	30.0	41.0
51	34.51755168778899	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	7.0
11	3.0
12	9.0
13	9.0
14	7.0
15	14.0
16	21.0
17	41.0
18	125.0
19	324.0
20	724.0
21	1258.0
22	1542.0
23	1718.0
24	1963.0
25	2516.0
26	3037.0
27	3321.0
28	3664.0
29	4207.0
30	5176.0
31	7098.0
32	9634.0
33	13949.0
34	24363.0
35	27358.0
36	26870.0
37	40158.0
38	71261.0
39	83534.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.17668479432692	18.015260774777797	20.791607771735222	11.016446659160058
2	28.886167077932967	34.58260463333413	20.67062360748425	15.860604681248653
3	28.53010229750126	33.94504204499174	23.490992070146856	14.033863587360148
4	26.075381519369444	21.133897127524495	35.94906686471335	16.841654488392706
5	32.99783186794758	25.18357250664814	22.531803262978844	19.286792362425434
6	23.622757001509306	40.0238973670971	23.190328454037996	13.163017177355599
7	70.17890084089984	5.414939747490477	19.789894827627514	4.616264583982176
8	68.28328262379915	14.611233559330156	11.23805131645145	5.867432500419252
9	63.29448264296496	6.630471239309073	11.47253300112599	18.602513116599987
10	42.65200881627177	21.367779880692844	19.919563498718286	16.0606478043171
11	33.77644282599842	21.860999976042738	24.416940178721163	19.94561701923768
12	26.992645121103955	19.62788385520232	29.027215447641407	24.352255576052322
13	25.118288972473106	22.906135454349442	34.161855250233586	17.81372032294387
14	20.853297716873097	30.635945473275676	26.086461752234015	22.42429505761721
15	18.05419132268034	23.293943604609378	37.967466040583595	20.684399032126684
16	19.694065786636642	24.04410531611605	27.80509570925469	28.456733187992622
17	20.02138185477109	25.534546369277212	31.322021513619703	23.122050262332
18	20.623308018495003	24.504983110131526	29.772585707098536	25.099123164274932
19	21.15725545626602	25.863060300424046	26.806976354184137	26.172707889125803
20	24.389389329436288	25.548022328166553	31.930835389664836	18.131752952732324
21	22.241920413981457	30.822812103207873	27.797908531180376	19.137358951630294
22	20.429553676241586	22.52311875613905	30.46974198030713	26.577585587312235
23	21.695694880333484	28.31688268129657	29.023322392851153	20.964100045518794
24	23.984212165496753	23.0708416185525	26.859682326729118	26.085263889221626
25	18.867839773843464	32.91068733379651	25.356663711937905	22.864809180422128
26	19.212524855657506	23.51614719340696	31.85087803358808	25.420449917347455
27	23.86891785055461	26.744986943293164	27.128303107256652	22.25779209889557
28	18.74056682877746	26.057713039936754	32.244974964663044	22.956745166622746
29	21.989770249874223	22.108957619606624	33.00262331999713	22.89864881052203
30	19.85996981385209	27.042356436117963	32.344996526197264	20.752677223832684
31	26.640772861215588	23.87041517932009	24.70861982223713	24.780192137227186
32	25.835808916892265	26.628794231091735	28.42139622912724	19.114000622888767
33	23.666479001461393	22.90523705709015	24.330993507582473	29.097290433865986
34	23.46853213866462	25.40338036942095	29.605483816870702	21.522603675043722
35	23.982714836731272	22.666263386119162	29.071536379099687	24.27948539804988
36	26.602740710572338	25.501006204930405	27.3535013535852	20.542751730912055
37	21.741812606310344	24.107592055772503	35.35552574207614	18.795069595841017
38	25.32551927361587	23.986607891521526	23.782671713662825	26.905201121199777
39	23.890479384777557	25.620193574662803	29.170659543374622	21.31866749718502
40	23.663184878177333	21.757684291224454	28.802017201312857	25.777113629285353
41	23.71529191921612	28.032390215854914	24.305538918569273	23.946778946359697
42	24.67777484966819	21.796614839127	31.004887281090532	22.520723030114276
43	28.15427277736518	21.21265662058887	26.385328573824296	24.24774202822165
44	22.873793153015022	21.94245466088498	29.83307778922402	25.350674396875974
45	22.097877386742052	20.45291200498311	28.157866366402338	29.291344241872498
46	27.993459667952376	24.345367863731106	26.930356244459887	20.730816223856642
47	19.391006444503006	22.30540715363791	36.3272921108742	21.97629429098488
48	22.9944778515129	24.44419156225294	27.01899810737644	25.542332478857716
49	22.324273496082988	19.84290026592559	34.87099015356604	22.961836084425386
50	23.803035384873386	20.687992621163843	29.348841666467024	26.160130327495747
51	21.340528497161067	20.976677607148847	27.03367192927817	30.64912196641192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	198.0
2	318.0
3	1145.0
4	1972.0
5	1403.0
6	834.0
7	777.5
8	721.0
9	707.5
10	694.0
11	707.5
12	721.0
13	699.0
14	677.0
15	626.0
16	575.0
17	607.0
18	639.0
19	623.0
20	607.0
21	689.5
22	772.0
23	931.5
24	1091.0
25	1189.0
26	1480.5
27	1674.0
28	2183.5
29	2693.0
30	3388.0
31	4083.0
32	4685.5
33	5288.0
34	6060.0
35	6832.0
36	7193.5
37	7555.0
38	8610.5
39	9666.0
40	10888.0
41	12110.0
42	13658.0
43	15206.0
44	17607.5
45	20009.0
46	40070.5
47	60132.0
48	47149.5
49	34167.0
50	33579.0
51	32991.0
52	28347.5
53	23704.0
54	21424.5
55	19145.0
56	16657.0
57	14169.0
58	13491.5
59	12814.0
60	12383.5
61	11953.0
62	10832.0
63	9711.0
64	8278.5
65	6846.0
66	5615.5
67	4385.0
68	3531.0
69	2677.0
70	2366.5
71	2056.0
72	1588.5
73	1121.0
74	1018.5
75	785.0
76	654.0
77	437.5
78	221.0
79	161.0
80	101.0
81	72.5
82	44.0
83	26.0
84	8.0
85	8.0
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	333928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.486825814505753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.84308431811934	21.902675975833542
2	10.19088958950604	6.213757516202614
3	3.5024081625616863	3.2033192274996383
4	1.9640382886359227	2.395091727946534
5	1.3373151038667004	2.0385246315345884
6	1.021475248929673	1.8684922792768708
7	0.8695531980679029	1.8556941820159802
8	0.8215778428485995	2.003784047038611
9	0.7446173875791362	2.0430918534219704
>10	7.586101975162444	40.19055722294073
>50	0.08095840052542663	1.6530608955049664
>100	0.02598664708223571	1.7722031646556466
>500	9.99486426239835E-4	0.22640078255487373
>1k	0.00799589140991868	4.181558464334498
>5k	0.0029984592787195047	8.451788029238939
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	9815	2.939256366641911	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	9017	2.700282695670923	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	8905	2.6667425313241178	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3024	0.9055844373637431	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	2183	0.6537337390096069	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	1985	0.5944395198965047	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	1493	0.4471023693730385	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1470	0.4402146570518195	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	1445	0.4327280132244077	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1096	0.3282144653937376	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1027	0.30755132843008076	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	743	0.22250305455068156	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	472	0.14134783546153662	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG	444	0.1329627943748353	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG	409	0.12248149301645865	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT	358	0.10720873960853837	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	357	0.1069092738554419	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	348	0.1042140820775736	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9946575309647587E-4	0.0	0.0	0.2308880956373829	0.0
2	2.9946575309647587E-4	0.0	0.0	1.7276179296135694	0.0
3	2.9946575309647587E-4	0.0	0.0	2.101650655231068	0.0
4	2.9946575309647587E-4	0.0	0.0	2.9407536954073934	0.0
5	2.9946575309647587E-4	0.0	0.0	6.846685513044728	0.0
6	2.9946575309647587E-4	0.0	0.0	7.597446156057593	0.0
7	2.9946575309647587E-4	0.0	0.0	8.885747825878633	0.0
8	2.9946575309647587E-4	0.0	0.0	10.267782276418869	0.0
9	2.9946575309647587E-4	0.0	0.0	10.6391198102585	0.0
10	2.9946575309647587E-4	0.0	0.0	14.161435998179249	0.0
11	2.9946575309647587E-4	0.0	0.0	15.445844613210033	0.0
12	2.9946575309647587E-4	0.0	0.0	19.484739225222203	0.0
13	2.9946575309647587E-4	0.0	0.0	20.040547662969264	0.0
14	2.9946575309647587E-4	0.0	0.0	20.322644402386143	0.0
15	2.9946575309647587E-4	0.0	0.0	21.104250017967946	0.0
16	2.9946575309647587E-4	0.0	0.0	21.859203181524162	0.0
17	2.9946575309647587E-4	0.0	0.0	22.653685824489113	0.0
18	2.9946575309647587E-4	0.0	0.0	23.41193311132939	0.0
19	2.9946575309647587E-4	0.0	0.0	24.646031479839966	0.0
20	2.9946575309647587E-4	0.0	0.0	25.25933734218155	0.0
21	2.9946575309647587E-4	0.0	0.0	25.83940250592942	0.0
22	2.9946575309647587E-4	0.0	0.0	26.594655135238735	0.0
23	2.9946575309647587E-4	0.0	0.0	27.182506408567118	0.0
24	2.9946575309647587E-4	0.0	0.0	27.699384298411633	0.0
25	2.9946575309647587E-4	0.0	0.0	28.134208571907717	0.0
26	2.9946575309647587E-4	0.0	0.0	28.559449941304713	0.0
27	2.9946575309647587E-4	0.0	0.0	29.07213531060588	0.0
28	2.9946575309647587E-4	0.0	0.0	29.482103926594956	0.0
29	5.989315061929517E-4	0.0	0.0	29.891773076830933	0.0
30	5.989315061929517E-4	0.0	0.0	30.360736446180013	0.0
31	5.989315061929517E-4	0.0	0.0	30.82371050046717	0.0
32	5.989315061929517E-4	0.0	0.0	31.280994705445487	0.0
33	5.989315061929517E-4	0.0	0.0	31.706535540595578	0.0
34	5.989315061929517E-4	0.0	0.0	32.13566996478283	0.0
35	5.989315061929517E-4	0.0	0.0	32.56690064924175	0.0
36	5.989315061929517E-4	0.0	0.0	32.986452169329915	0.0
37	5.989315061929517E-4	0.0	0.0	33.397019716825184	0.0
38	5.989315061929517E-4	0.0	0.0	33.82465681224695	0.0
39	5.989315061929517E-4	0.0	0.0	34.23193023645816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	45	3.8380676E-10	45.000004	41
GACGAAT	30	2.1608594E-6	45.000004	20
GTACGAG	30	2.1608594E-6	45.000004	1
AAGTACG	20	7.0260896E-4	45.0	1
CGAACCC	35	1.2084638E-7	45.0	34
GCGAACC	35	1.2084638E-7	45.0	33
TCTAGCG	20	7.0260896E-4	45.0	1
CCGTAAG	20	7.0260896E-4	45.0	42
TGTTCGG	25	3.884842E-5	45.0	2
TCAGACG	20	7.0260896E-4	45.0	22
CGTAAGG	40	6.7902874E-9	45.0	2
CGTAAGC	25	3.884842E-5	45.0	43
CGCACGG	25	3.884842E-5	45.0	2
GCTACGA	40	6.7902874E-9	45.0	10
GTAAGCG	25	3.884842E-5	45.0	1
ATACAGG	20	7.0260896E-4	45.0	2
GGCTACG	20	7.0260896E-4	45.0	1
TGCAACG	20	7.0260896E-4	45.0	1
CCGATGA	555	0.0	43.783783	18
TACGGCT	1075	0.0	42.488373	7
>>END_MODULE