##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457899
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29265405689901	31.0	31.0	33.0	28.0	34.0
2	31.210247237928016	31.0	31.0	34.0	28.0	34.0
3	31.154951201029046	31.0	31.0	34.0	28.0	34.0
4	35.07646882827873	35.0	35.0	37.0	32.0	37.0
5	35.27502353139011	35.0	35.0	37.0	33.0	37.0
6	35.16858739591045	36.0	35.0	37.0	33.0	37.0
7	35.5006693615841	37.0	35.0	37.0	33.0	37.0
8	34.98636380511859	37.0	35.0	37.0	32.0	37.0
9	36.82697057648084	39.0	37.0	39.0	32.0	39.0
10	36.41932609592945	37.0	35.0	39.0	32.0	39.0
11	36.62814725518073	38.0	35.0	39.0	32.0	39.0
12	36.73956265464655	39.0	35.0	39.0	33.0	39.0
13	36.64564674742683	39.0	35.0	39.0	32.0	39.0
14	37.6502132566352	39.0	36.0	41.0	33.0	41.0
15	37.686053037897004	40.0	36.0	41.0	32.0	41.0
16	37.86308989536994	39.0	36.0	41.0	33.0	41.0
17	37.63112171024615	39.0	36.0	41.0	32.0	41.0
18	37.61763183584153	39.0	36.0	41.0	33.0	41.0
19	37.532198148499994	39.0	36.0	41.0	32.0	41.0
20	37.42257135307131	39.0	36.0	41.0	32.0	41.0
21	37.524360175497215	39.0	36.0	41.0	32.0	41.0
22	37.675308310347916	39.0	36.0	41.0	33.0	41.0
23	37.72374475593963	39.0	36.0	41.0	33.0	41.0
24	37.56928492964606	39.0	36.0	41.0	33.0	41.0
25	37.39278094077515	39.0	35.0	41.0	32.0	41.0
26	37.377550507863084	39.0	35.0	41.0	32.0	41.0
27	37.41249707905018	39.0	36.0	41.0	33.0	41.0
28	37.235942860761874	39.0	35.0	41.0	32.0	41.0
29	37.29537736487741	39.0	35.0	41.0	32.0	41.0
30	37.06551663139688	39.0	35.0	41.0	31.0	41.0
31	37.04092605574592	39.0	35.0	41.0	31.0	41.0
32	36.93719138936753	39.0	35.0	41.0	31.0	41.0
33	36.89521269974383	39.0	35.0	41.0	31.0	41.0
34	36.87306371055626	39.0	35.0	41.0	31.0	41.0
35	36.84009355774963	39.0	35.0	41.0	31.0	41.0
36	36.73312236978023	39.0	35.0	41.0	31.0	41.0
37	36.75160679538501	39.0	35.0	41.0	31.0	41.0
38	36.5635959021531	39.0	35.0	41.0	30.0	41.0
39	36.591460125486186	39.0	35.0	41.0	31.0	41.0
40	36.46102743181357	39.0	35.0	41.0	30.0	41.0
41	36.203254429470256	39.0	35.0	40.0	30.0	41.0
42	36.29658723867054	39.0	35.0	41.0	30.0	41.0
43	36.33958580385631	39.0	35.0	40.0	30.0	41.0
44	36.33126955944433	39.0	35.0	40.0	30.0	41.0
45	36.27477238430309	39.0	35.0	40.0	30.0	41.0
46	36.16765924363233	39.0	35.0	40.0	30.0	41.0
47	36.01044771882009	38.0	35.0	40.0	30.0	41.0
48	35.97504471510093	38.0	35.0	40.0	30.0	41.0
49	36.00468662303259	38.0	35.0	40.0	30.0	41.0
50	35.891183426912924	38.0	35.0	40.0	29.0	41.0
51	34.712971637850266	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	9.0
11	9.0
12	6.0
13	11.0
14	14.0
15	22.0
16	22.0
17	68.0
18	150.0
19	299.0
20	657.0
21	1227.0
22	1769.0
23	2382.0
24	2844.0
25	3486.0
26	4041.0
27	4565.0
28	5103.0
29	5877.0
30	7709.0
31	10294.0
32	13894.0
33	19397.0
34	32732.0
35	36494.0
36	38133.0
37	56800.0
38	98442.0
39	111409.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.0405111170804	17.896959809914414	20.827300343525536	11.235228729479644
2	28.658284905623294	33.08327819016858	21.6344652423351	16.623971661873032
3	28.95136263673867	32.61439749813824	23.80153702017257	14.632702844950524
4	26.711785786821984	22.41935448646972	33.448642604591846	17.420217122116448
5	32.2560215243973	26.40407600802797	21.74846418096567	19.591438286609055
6	24.001362745933054	39.91819156626243	22.74802958731074	13.332416100493777
7	71.16787763240366	5.7416591868512485	18.05485489158089	5.035608289164204
8	68.83897977501589	13.786446356074155	10.80609479383008	6.5684790750798765
9	63.11479168987047	7.4837464156942906	12.021428306242205	17.38003358819303
10	42.408260336886514	22.33418286565378	19.402968776957362	15.854588020502339
11	33.72009984734625	22.624421542741956	24.020799346580795	19.634679263330995
12	28.603687712792556	19.0795350066281	28.292920491200025	24.023856789379316
13	25.922747156032226	22.24049408275624	33.32481617125174	18.511942589959794
14	20.583796863500467	30.016881452023263	26.131745210188274	23.267576474288
15	17.358194711060737	23.219530944596954	38.080231666808615	21.342042677533694
16	18.865514010731623	24.05202894087998	29.621597775928755	27.460859272459647
17	19.410612383953666	25.545152970414875	30.803299417557145	24.240935228074314
18	19.8089535028467	25.216914647116507	29.111659994889706	25.862471855147096
19	21.76025717461711	25.68339306266229	26.723797169244744	25.832552593475853
20	25.247489075101715	25.08238716398158	31.152939840445164	18.517183920471545
21	23.003107672215926	29.855273761244295	26.888462302822237	20.253156263717546
22	19.440094868082262	23.523528114278477	29.364772580852982	27.67160443678628
23	22.440101419745403	28.45802240232016	28.046796345919077	21.05507983201536
24	23.95921371306773	23.949167829586873	26.06164241459361	26.029976042751784
25	19.727712879914566	31.84042769256976	24.687103487887065	23.744755939628607
26	19.83887276451794	24.7997920938897	28.63120469797925	26.73013044361311
27	25.454303241544533	26.636660049486892	25.35580990567789	22.553226803290684
28	18.09765909076019	26.542971266589355	31.053354560721907	24.306015081928546
29	22.54514641875173	22.515445545851815	26.4377078788117	28.501700156584747
30	23.84761705092171	26.193112454930016	27.062518153566618	22.896752340581656
31	22.557157801174498	23.368472086639194	26.592327128908337	27.48204298327797
32	24.551484060895525	27.817706524801324	22.479848176126175	25.15096123817698
33	22.259275517090014	24.634471793998237	23.75676732205137	29.349485366860378
34	22.032587972456806	23.504091513630733	28.384643775155656	26.0786767387568
35	22.647133974959544	25.518946317856123	23.773364868671912	28.060554838512424
36	20.420660451322235	28.2612541193582	25.303178211789064	26.014907217530503
37	22.799132559800306	24.771838331160364	29.277417072323807	23.151612036715523
38	19.877090799499452	25.677714954607893	25.95747097067257	28.487723275220084
39	22.27609145248188	23.075831133066462	27.562191662353484	27.08588575209817
40	20.533130668553547	23.98301808914193	29.17499273857335	26.30885850373117
41	18.20052020205329	28.18263416168194	28.406701041059275	25.21014459520549
42	20.26189181457046	26.630108386347207	29.240072592427584	23.867927206654745
43	22.837132206010498	26.291605790796662	24.699770036623796	26.17149196656905
44	22.649536251444097	22.762880023760697	28.308862871506594	26.278720853288608
45	20.50080913039775	21.24158384272514	27.35319360819744	30.904413418679667
46	25.522877315739933	25.133708525242465	25.317373481925053	24.026040677092546
47	19.053328354069347	23.075831133066462	34.15425672473624	23.71658378812795
48	21.044378782220534	24.285049759881545	27.321090458812968	27.349480999084953
49	19.535749149921706	22.00812843006864	33.243793937090935	25.21232848291872
50	21.013804354235322	22.017300758464202	29.27654351723852	27.692351370061957
51	19.539461759034197	21.677924607828363	26.38900718280669	32.39360645033075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	167.0
1	337.5
2	508.0
3	1359.0
4	2210.0
5	1564.5
6	919.0
7	907.0
8	895.0
9	892.5
10	890.0
11	888.0
12	886.0
13	901.0
14	916.0
15	854.0
16	792.0
17	816.5
18	841.0
19	853.0
20	865.0
21	1057.5
22	1250.0
23	1369.5
24	1489.0
25	1708.5
26	2298.0
27	2668.0
28	3333.0
29	3998.0
30	5100.0
31	6202.0
32	6667.5
33	7133.0
34	7981.5
35	8830.0
36	9965.5
37	11101.0
38	12035.0
39	12969.0
40	14425.5
41	15882.0
42	18032.0
43	20182.0
44	22900.0
45	25618.0
46	50267.0
47	74916.0
48	60184.5
49	45453.0
50	43851.5
51	42250.0
52	36974.0
53	31698.0
54	28285.0
55	24872.0
56	23384.0
57	21896.0
58	21328.5
59	20761.0
60	19788.0
61	18815.0
62	17652.5
63	16490.0
64	13413.0
65	10336.0
66	8712.0
67	7088.0
68	6016.5
69	4945.0
70	4136.5
71	3328.0
72	2990.0
73	2652.0
74	1992.5
75	1155.5
76	978.0
77	760.0
78	542.0
79	355.0
80	168.0
81	147.5
82	127.0
83	105.5
84	84.0
85	49.0
86	14.0
87	9.5
88	5.0
89	5.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.12065190731302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79408254605198	21.92605221281842
2	10.41356970590051	6.273270164479388
3	3.726910844985365	3.3677095275418205
4	1.9152605844140427	2.3075558949972916
5	1.1911389017917768	1.7938940117064612
6	0.8358680177207265	1.5106133761333054
7	0.6748156576050635	1.4228121267028655
8	0.6054582665493721	1.4589438152911023
9	0.520333807749505	1.4105514148946616
>10	7.167783373468489	42.78540505158405
>50	0.10245887051928407	2.0709888572633015
>100	0.041419543401412705	2.2933651708312066
>500	0.0029066346246605407	0.59293054473962
>1k	0.005813269249321081	3.6372535225112608
>5k	7.266586561651352E-4	2.1556932207975334
>10k+	0.0014533173123302703	4.9929610877077195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	12056	2.632895027069288	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	10756	2.3489896243494743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	9849	2.1509110087595738	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	3882	0.8477852102756286	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCCTAAT	2410	0.5263169388882701	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCT	2238	0.48875407022072553	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCT	2125	0.464076139061234	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTC	1844	0.4027088943194897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTA	1824	0.398341118893031	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1189	0.2596642491029681	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1106	0.24153798108316463	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	858	0.18737756579507708	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	747	0.16313641217823144	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTT	562	0.12273448948348872	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATG	542	0.11836671405703005	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGT	460	0.10045883480854949	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.367775426458673E-4	0.0	0.0	0.19698667173328616	0.0
2	4.367775426458673E-4	0.0	0.0	1.5153996842098367	0.0
3	4.367775426458673E-4	0.0	0.0	1.870063048838281	0.0
4	4.367775426458673E-4	0.0	0.0	2.762836346006434	0.0
5	4.367775426458673E-4	0.0	0.0	6.32169976348496	0.0
6	4.367775426458673E-4	0.0	0.0	7.085623685572583	0.0
7	4.367775426458673E-4	0.0	0.0	8.252038113208371	0.0
8	4.367775426458673E-4	0.0	0.0	9.769403296360114	0.0
9	4.367775426458673E-4	0.0	0.0	10.178227076276647	0.0
10	4.367775426458673E-4	0.0	0.0	13.216233274149976	0.0
11	4.367775426458673E-4	0.0	0.0	14.523290070517735	0.0
12	4.367775426458673E-4	0.0	0.0	18.268220721163402	0.0
13	4.367775426458673E-4	0.0	0.0	18.853721017080186	0.0
14	4.367775426458673E-4	0.0	0.0	19.124086315977976	0.0
15	4.367775426458673E-4	0.0	0.0	19.889102181922215	0.0
16	4.367775426458673E-4	0.0	0.0	20.66132487732011	0.0
17	4.367775426458673E-4	0.0	0.0	21.46390361193189	0.0
18	4.367775426458673E-4	0.0	0.0	22.26036746094663	0.0
19	4.367775426458673E-4	0.0	0.0	23.384196078174444	0.0
20	4.367775426458673E-4	0.0	0.0	23.95550110395524	0.0
21	4.367775426458673E-4	0.0	0.0	24.593414704989527	0.0
22	4.367775426458673E-4	0.0	0.0	25.34248819062719	0.0
23	4.367775426458673E-4	0.0	0.0	26.021895658212838	0.0
24	4.367775426458673E-4	0.0	0.0	26.52768405259675	0.0
25	4.367775426458673E-4	0.0	0.0	27.00530029548001	0.0
26	6.551663139688009E-4	0.0	0.0	27.45605471949054	0.0
27	6.551663139688009E-4	0.0	0.0	27.99350948571628	0.0
28	6.551663139688009E-4	0.0	0.0	28.428976695734214	0.0
29	6.551663139688009E-4	0.0	0.0	28.866627793465373	0.0
30	6.551663139688009E-4	0.0	0.0	29.374600075562515	0.0
31	6.551663139688009E-4	0.0	0.0	29.859859925442073	0.0
32	6.551663139688009E-4	0.0	0.0	30.361498933170854	0.0
33	6.551663139688009E-4	0.0	0.0	30.827540571173994	0.0
34	6.551663139688009E-4	0.0	0.0	31.263881336277215	0.0
35	6.551663139688009E-4	0.0	0.0	31.726210365167866	0.0
36	6.551663139688009E-4	0.0	0.0	32.20426338559377	0.0
37	6.551663139688009E-4	0.0	0.0	32.69935073018286	0.0
38	6.551663139688009E-4	0.0	0.0	33.14289832473973	0.0
39	8.735550852917346E-4	0.0	0.0	33.60107796697525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCCG	20	7.0288347E-4	45.000004	45
GTACATC	20	7.0288347E-4	45.000004	36
TGACGGA	20	7.0288347E-4	45.000004	44
GCGTTCG	20	7.0288347E-4	45.000004	44
CGTTCGG	20	7.0288347E-4	45.000004	45
TCTAGCG	20	7.0288347E-4	45.000004	1
GTAGCGA	20	7.0288347E-4	45.000004	29
AAGCCGT	20	7.0288347E-4	45.000004	25
TTAAGCG	20	7.0288347E-4	45.000004	1
ACGTGCT	20	7.0288347E-4	45.000004	30
CGTACAT	20	7.0288347E-4	45.000004	35
TCGTACA	20	7.0288347E-4	45.000004	34
CGGTCTA	30	2.1626292E-6	45.000004	31
CACACGA	30	2.1626292E-6	45.000004	13
TCGACGT	20	7.0288347E-4	45.000004	26
GGTTACG	20	7.0288347E-4	45.000004	1
TAACTAT	20	7.0288347E-4	45.000004	28
GTGATAG	55	1.8189894E-12	45.0	1
GTAGATG	25	3.8871167E-5	45.0	1
TGTAGCG	35	1.2097917E-7	45.0	1
>>END_MODULE