SRR3550963_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270304
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	8085	2.9910767136261396	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	6569	2.430226707706878	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	6462	2.3906416479223394	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2478	0.9167455901503493	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	1546	0.5719486208121226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	1451	0.5368030069847283	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	1044	0.3862317982715757	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	1026	0.3795726293358589	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	998	0.3692139221025216	RNA PCR Primer, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	836	0.3092814016810702	RNA PCR Primer, Index 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.2726559725346277	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	498	0.18423700722149874	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	328	0.12134485616195098	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGGATATCGT	294	0.10876642595004143	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	285	0.10543684148218303	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGGATA	284	0.10506688765242098	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATG	283	0.10469693382265893	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.2073178E-7	45.0	21
AGCGTAA	25	3.8828643E-5	45.0	44
CTATCGT	20	7.023706E-4	45.0	41
GACACGA	35	1.2073178E-7	45.0	25
ACCGCTC	20	7.023706E-4	45.0	18
TAAACGG	20	7.023706E-4	45.0	2
AGGGTTC	35	1.2073178E-7	45.0	6
AATTGCG	20	7.023706E-4	45.0	1
TCTCGTC	20	7.023706E-4	45.0	34
TAGGGTC	25	3.8828643E-5	45.0	5
CATCGTA	25	3.8828643E-5	45.0	42
CGTAACT	20	7.023706E-4	45.0	33
ATGCGCG	20	7.023706E-4	45.0	1
TAGGCGG	20	7.023706E-4	45.0	2
CGCGTAA	20	7.023706E-4	45.0	31
CACAACG	20	7.023706E-4	45.0	12
GCCAATC	20	7.023706E-4	45.0	15
TCGAACG	20	7.023706E-4	45.0	1
GCGTAAT	25	3.8828643E-5	45.0	45
GCGTAAC	20	7.023706E-4	45.0	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content