##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550963_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270304
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268519888717886	31.0	31.0	33.0	28.0	34.0
2	31.209493755179352	31.0	31.0	34.0	28.0	34.0
3	31.161507043920917	31.0	31.0	34.0	28.0	34.0
4	35.064453356221144	35.0	35.0	37.0	32.0	37.0
5	35.25735468213567	35.0	35.0	37.0	33.0	37.0
6	35.15861030543388	36.0	35.0	37.0	33.0	37.0
7	35.497084763821476	37.0	35.0	37.0	33.0	37.0
8	34.99245664141115	37.0	35.0	37.0	32.0	37.0
9	36.81780513791879	39.0	37.0	39.0	32.0	39.0
10	36.3928798685924	37.0	35.0	39.0	32.0	39.0
11	36.56562241032319	38.0	35.0	39.0	32.0	39.0
12	36.617811797087725	39.0	35.0	39.0	32.0	39.0
13	36.50581197466556	39.0	35.0	39.0	32.0	39.0
14	37.5113501834971	39.0	36.0	41.0	32.0	41.0
15	37.51540117793299	39.0	36.0	41.0	32.0	41.0
16	37.77692523973008	39.0	36.0	41.0	33.0	41.0
17	37.47411063099325	39.0	36.0	41.0	32.0	41.0
18	37.51660722741802	39.0	36.0	41.0	33.0	41.0
19	37.38581005090565	39.0	36.0	40.0	32.0	41.0
20	37.18197288978335	39.0	35.0	41.0	32.0	41.0
21	37.36492245767728	39.0	35.0	40.0	32.0	41.0
22	37.48217192494376	39.0	35.0	41.0	33.0	41.0
23	37.50088788919143	39.0	35.0	41.0	33.0	41.0
24	37.42498816147745	39.0	35.0	41.0	32.0	41.0
25	37.25354045815082	39.0	35.0	41.0	32.0	41.0
26	37.227791671599384	39.0	35.0	41.0	32.0	41.0
27	37.19881318811412	39.0	35.0	41.0	32.0	41.0
28	37.06005830472357	39.0	35.0	41.0	32.0	41.0
29	37.06870782526341	39.0	35.0	41.0	31.0	41.0
30	36.84806736119332	39.0	35.0	41.0	31.0	41.0
31	36.80342133301764	39.0	35.0	41.0	31.0	41.0
32	36.701880105362854	39.0	35.0	41.0	31.0	41.0
33	36.693900201254884	39.0	35.0	41.0	31.0	41.0
34	36.5079207114952	39.0	35.0	41.0	30.0	41.0
35	36.48264916538416	39.0	35.0	41.0	30.0	41.0
36	36.46032985083462	39.0	35.0	41.0	30.0	41.0
37	36.49127648869421	39.0	35.0	41.0	31.0	41.0
38	36.161015005327336	39.0	35.0	41.0	30.0	41.0
39	36.200825736948026	39.0	35.0	41.0	30.0	41.0
40	36.09150068071504	39.0	35.0	40.0	30.0	41.0
41	35.86501864567302	38.0	35.0	40.0	28.0	41.0
42	36.00805389487392	39.0	35.0	40.0	29.0	41.0
43	35.99141337161122	39.0	35.0	40.0	30.0	41.0
44	36.0245686338345	39.0	35.0	40.0	30.0	41.0
45	35.95005623298212	38.0	35.0	40.0	29.0	41.0
46	35.82903693619036	38.0	35.0	40.0	29.0	41.0
47	35.640467769622354	38.0	35.0	40.0	28.0	41.0
48	35.62470773647449	38.0	35.0	40.0	28.0	41.0
49	35.683245530957734	38.0	35.0	40.0	29.0	41.0
50	35.510395702616314	38.0	35.0	40.0	28.0	41.0
51	34.213045311945066	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	3.0
11	3.0
12	6.0
13	7.0
14	13.0
15	18.0
16	31.0
17	40.0
18	122.0
19	327.0
20	619.0
21	1146.0
22	1427.0
23	1588.0
24	1782.0
25	2201.0
26	2604.0
27	2891.0
28	3191.0
29	3748.0
30	4751.0
31	6227.0
32	8409.0
33	11705.0
34	20645.0
35	22852.0
36	22818.0
37	32682.0
38	56085.0
39	62346.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.14796673375162	17.81290694921274	20.19171007458269	10.847416242452942
2	28.951106901858648	33.448265656446075	21.499496862791524	16.101130578903753
3	28.19048182786788	33.348748076240085	23.93897241624245	14.521797679649579
4	26.3928761690541	21.621581626612997	35.433067953119455	16.552474251213447
5	32.70613827394342	25.537912868474017	22.849088433763466	18.906860423819108
6	23.527583757547056	40.046392210252165	22.763629099088433	13.662394933112349
7	70.72074405114242	5.341393394104416	19.489907659524093	4.447954895229075
8	68.59276962235114	14.569521723688883	10.690925772463597	6.146782881496389
9	63.48185746418847	6.865603172724044	11.56845625665917	18.084083106428317
10	44.10182609210371	20.79843435539245	19.52949271930863	15.570246833195217
11	35.13673493548005	22.180951817213213	23.465431514147035	19.216881733159703
12	27.578578193441462	20.33821179116846	27.41283887770806	24.670371137682018
13	25.330368769977508	22.650793181011007	33.992837693855805	18.026000355155677
14	21.078119450692554	30.810124896412926	25.9259944358944	22.18576121700012
15	17.7733218894282	22.63414525867172	38.0634396827276	21.529093169172487
16	20.00007399076595	23.60231443115899	27.978868237243994	28.418743340831064
17	20.273839824789867	25.533103468687106	30.893734461939147	23.299322244583877
18	21.21130282940689	23.919364863265063	29.62775245649343	25.24157985083462
19	21.022256422398485	25.401029951462057	26.690319048182786	26.886394577956672
20	25.711421214632413	25.063632058719072	31.366905410204804	17.858041316443707
21	23.065141470344503	30.92481058363916	26.995900911566235	19.0141470344501
22	20.748490588374572	22.75586006866343	30.147907541138867	26.347741801823133
23	22.300816858056113	28.26891203977744	27.822747721084408	21.607523381082043
24	23.706271457322124	24.120619746655617	26.641115188824433	25.531993607197823
25	19.601263762282468	32.798626731383926	24.43914999408074	23.16095951225287
26	21.03261512963182	23.81873742156979	29.736888836273234	25.41175861252516
27	24.174633005800878	26.494613472238665	25.295963063809634	24.034790458150823
28	18.09185213685332	27.63148159109743	30.701728424292646	23.5749378477566
29	26.615218420741094	22.35593997869066	26.70141766307565	24.3274239374926
30	21.383701314076003	28.04361015745235	27.294823606013967	23.277864922457677
31	26.160175210133772	27.371034094944953	21.84947318574642	24.619317509174856
32	29.740218420741094	27.01957795667101	23.136172605658814	20.104031016929085
33	25.62818160293595	25.416937966141827	22.093272759559607	26.861607671362613
34	26.72472475435066	27.090609091985318	24.96929383212975	21.21537232153427
35	23.3907008405351	23.776562684976916	26.21862791523618	26.614108559251804
36	25.965209541849177	31.30216348999645	22.653752811649106	20.078874156505268
37	23.361104534154137	26.389176630756484	27.307032082396116	22.942686752693263
38	23.495767728187523	29.89041967562448	22.577912276547888	24.035900319640106
39	28.15866579850835	27.373623771753287	24.3888362732331	20.078874156505268
40	25.63336095655262	22.532777909316916	29.732079436486323	22.101781697644135
41	19.764413401207527	32.437551793536166	23.451003314786316	24.347031490469988
42	20.654892269444776	23.734757902213804	32.16785545163964	23.442494376701788
43	25.570098851663314	26.389546584586242	23.747706286255475	24.29264827749497
44	22.514280217828812	23.816517698591216	27.799070675979635	25.870131407600333
45	21.28936308748668	22.413282822303778	26.651843849887534	29.64551024032201
46	29.21895347460637	25.100257487865512	25.060672428080977	20.62011660944714
47	20.18209127500888	23.7776725464662	34.52446134722387	21.515774831301055
48	22.571993015271694	26.171273825026635	25.854963300580085	25.401769859121583
49	21.405898543861728	21.566458505978453	33.93327512726412	23.094367822895702
50	23.273795430330292	22.611578075056233	28.79498638569907	25.31964010891441
51	21.662646501716587	22.086983544453652	25.784302119095535	30.466067834734222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	190.5
2	303.0
3	882.0
4	1461.0
5	1037.0
6	613.0
7	584.0
8	555.0
9	556.0
10	557.0
11	551.5
12	546.0
13	540.0
14	534.0
15	534.5
16	535.0
17	538.0
18	541.0
19	589.0
20	637.0
21	657.5
22	678.0
23	675.0
24	672.0
25	905.5
26	1177.5
27	1216.0
28	1669.0
29	2122.0
30	2432.0
31	2742.0
32	3308.0
33	3874.0
34	4700.5
35	5527.0
36	5950.5
37	6374.0
38	6795.5
39	7217.0
40	8759.0
41	10301.0
42	11319.0
43	12337.0
44	13766.5
45	15196.0
46	30291.5
47	45387.0
48	35513.5
49	25640.0
50	26187.5
51	26735.0
52	23167.5
53	19600.0
54	17528.0
55	15456.0
56	14476.0
57	13496.0
58	12525.5
59	11555.0
60	11129.0
61	10703.0
62	9446.0
63	8189.0
64	7177.5
65	6166.0
66	5058.0
67	3950.0
68	3465.5
69	2981.0
70	2334.5
71	1688.0
72	1431.5
73	1175.0
74	983.0
75	656.0
76	521.0
77	409.5
78	298.0
79	213.0
80	128.0
81	91.0
82	54.0
83	39.0
84	24.0
85	13.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	270304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.42571327098378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89215954728003	23.96005978453889
2	9.184465133260314	5.956256659168935
3	3.140972982840453	3.0554486800047354
4	1.8425976633807957	2.389901740262815
5	1.1956918583424607	1.9385580679531196
6	0.9675063891931361	1.8823250858292884
7	0.8842186929536326	2.0069995264590976
8	0.8260313983205549	2.142772581981769
9	0.719925155166119	2.1009677992186573
>10	7.2437477181453085	40.01346631940334
>50	0.06275100401606426	1.3325736948028886
>100	0.027382256297918947	1.666272049248254
>500	0.0034227820372398684	0.9511512963182195
>1k	0.0057046367287331146	2.791301645554635
>5k	0.0034227820372398684	7.811945069255357
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	8085	2.9910767136261396	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	6569	2.430226707706878	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	6462	2.3906416479223394	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	2478	0.9167455901503493	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	1546	0.5719486208121226	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	1451	0.5368030069847283	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	1044	0.3862317982715757	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	1026	0.3795726293358589	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	998	0.3692139221025216	RNA PCR Primer, Index 23 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	836	0.3092814016810702	RNA PCR Primer, Index 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	737	0.2726559725346277	No Hit
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	498	0.18423700722149874	Illumina Paired End PCR Primer 2 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	328	0.12134485616195098	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGGATATCGT	294	0.10876642595004143	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	285	0.10543684148218303	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAGGATA	284	0.10506688765242098	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATG	283	0.10469693382265893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.699538297620457E-4	0.0	0.0	0.19792529892269445	0.0
2	3.699538297620457E-4	0.0	0.0	1.6892091866935006	0.0
3	3.699538297620457E-4	0.0	0.0	2.0391855096483957	0.0
4	3.699538297620457E-4	0.0	0.0	2.7816828459808214	0.0
5	3.699538297620457E-4	0.0	0.0	6.326210488930982	0.0
6	3.699538297620457E-4	0.0	0.0	7.008775304841956	0.0
7	3.699538297620457E-4	0.0	0.0	8.140834023913815	0.0
8	3.699538297620457E-4	0.0	0.0	9.448620812122646	0.0
9	3.699538297620457E-4	0.0	0.0	9.797487273588256	0.0
10	3.699538297620457E-4	0.0	0.0	12.951713626139458	0.0
11	3.699538297620457E-4	0.0	0.0	14.055655854149402	0.0
12	3.699538297620457E-4	0.0	0.0	18.031549662602107	0.0
13	3.699538297620457E-4	0.0	0.0	18.57242216171422	0.0
14	3.699538297620457E-4	0.0	0.0	18.825470581271457	0.0
15	3.699538297620457E-4	0.0	0.0	19.53134248845744	0.0
16	3.699538297620457E-4	0.0	0.0	20.271990055641055	0.0
17	7.399076595240914E-4	0.0	0.0	21.01929679176039	0.0
18	7.399076595240914E-4	0.0	0.0	21.703341422990412	0.0
19	7.399076595240914E-4	0.0	0.0	22.794705220788444	0.0
20	7.399076595240914E-4	0.0	0.0	23.333357996921983	0.0
21	7.399076595240914E-4	0.0	0.0	23.911965786669825	0.0
22	7.399076595240914E-4	0.0	0.0	24.559384988753404	0.0
23	7.399076595240914E-4	0.0	0.0	25.125784302119097	0.0
24	7.399076595240914E-4	0.0	0.0	25.608944003788327	0.0
25	7.399076595240914E-4	0.0	0.0	26.01256363205872	0.0
26	7.399076595240914E-4	0.0	0.0	26.390286492245767	0.0
27	7.399076595240914E-4	0.0	0.0	26.829051734343555	0.0
28	7.399076595240914E-4	0.0	0.0	27.18457736474488	0.0
29	7.399076595240914E-4	0.0	0.0	27.57376879365455	0.0
30	7.399076595240914E-4	0.0	0.0	28.010314312773765	0.0
31	7.399076595240914E-4	0.0	0.0	28.39062684976915	0.0
32	7.399076595240914E-4	0.0	0.0	28.786847401444298	0.0
33	7.399076595240914E-4	0.0	0.0	29.147182431632533	0.0
34	7.399076595240914E-4	0.0	0.0	29.552651829051733	0.0
35	7.399076595240914E-4	0.0	0.0	29.954791642003077	0.0
36	7.399076595240914E-4	0.0	0.0	30.361000947081806	0.0
37	7.399076595240914E-4	0.0	0.0	30.74538297620457	0.0
38	7.399076595240914E-4	0.0	0.0	31.13272463596543	0.0
39	7.399076595240914E-4	0.0	0.0	31.55336214040488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.2073178E-7	45.0	21
AGCGTAA	25	3.8828643E-5	45.0	44
CTATCGT	20	7.023706E-4	45.0	41
GACACGA	35	1.2073178E-7	45.0	25
ACCGCTC	20	7.023706E-4	45.0	18
TAAACGG	20	7.023706E-4	45.0	2
AGGGTTC	35	1.2073178E-7	45.0	6
AATTGCG	20	7.023706E-4	45.0	1
TCTCGTC	20	7.023706E-4	45.0	34
TAGGGTC	25	3.8828643E-5	45.0	5
CATCGTA	25	3.8828643E-5	45.0	42
CGTAACT	20	7.023706E-4	45.0	33
ATGCGCG	20	7.023706E-4	45.0	1
TAGGCGG	20	7.023706E-4	45.0	2
CGCGTAA	20	7.023706E-4	45.0	31
CACAACG	20	7.023706E-4	45.0	12
GCCAATC	20	7.023706E-4	45.0	15
TCGAACG	20	7.023706E-4	45.0	1
GCGTAAT	25	3.8828643E-5	45.0	45
GCGTAAC	20	7.023706E-4	45.0	32
>>END_MODULE