FastQCFastQC Report
Sat 18 Jun 2016
SRR3550958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375053
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC40921.0910457988604276No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC38301.0211890052872528No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG35500.9465328900182108No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC17950.4785990246711798TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC8330.2221019429253999TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT8140.21703599224642917No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT7640.2037045430912431No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT7040.1877068041050198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.18637365918950122No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT6470.1725089520681077TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA6020.16051064782844027No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC5650.15064537545360254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG207.0272037E-445.01
CGTTCTG207.0272037E-445.01
ACGGGTG351.2090095E-745.05
TCTACGG207.0272037E-445.02
CATAGCG207.0272037E-445.01
GGGCGTA207.0272037E-445.07
GTATACG207.0272037E-445.01
TATAGCG406.7939254E-945.01
TAGGACG207.0272037E-445.01
CGGTAGT253.8857637E-545.012
CGCCGGT207.0272037E-445.028
TCGAACG253.8857637E-545.01
TGCGTAG207.0272037E-445.01
CGATGAA1450.043.44827319
GTGCGAG556.002665E-1140.9090921
TACGGCT4750.040.736847
CTCGTAA1000.040.533
CTACGGG1000.040.53
CGTAACC1000.040.535
ATAGCGG800.039.3752