Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550958_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 375053 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC | 4092 | 1.0910457988604276 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC | 3830 | 1.0211890052872528 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG | 3550 | 0.9465328900182108 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC | 1795 | 0.4785990246711798 | TruSeq Adapter, Index 16 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC | 833 | 0.2221019429253999 | TruSeq Adapter, Index 16 (95% over 21bp) |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT | 814 | 0.21703599224642917 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT | 764 | 0.2037045430912431 | No Hit |
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT | 704 | 0.1877068041050198 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 699 | 0.18637365918950122 | No Hit |
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT | 647 | 0.1725089520681077 | TruSeq Adapter, Index 13 (95% over 22bp) |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA | 602 | 0.16051064782844027 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC | 565 | 0.15064537545360254 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGTACG | 20 | 7.0272037E-4 | 45.0 | 1 |
CGTTCTG | 20 | 7.0272037E-4 | 45.0 | 1 |
ACGGGTG | 35 | 1.2090095E-7 | 45.0 | 5 |
TCTACGG | 20 | 7.0272037E-4 | 45.0 | 2 |
CATAGCG | 20 | 7.0272037E-4 | 45.0 | 1 |
GGGCGTA | 20 | 7.0272037E-4 | 45.0 | 7 |
GTATACG | 20 | 7.0272037E-4 | 45.0 | 1 |
TATAGCG | 40 | 6.7939254E-9 | 45.0 | 1 |
TAGGACG | 20 | 7.0272037E-4 | 45.0 | 1 |
CGGTAGT | 25 | 3.8857637E-5 | 45.0 | 12 |
CGCCGGT | 20 | 7.0272037E-4 | 45.0 | 28 |
TCGAACG | 25 | 3.8857637E-5 | 45.0 | 1 |
TGCGTAG | 20 | 7.0272037E-4 | 45.0 | 1 |
CGATGAA | 145 | 0.0 | 43.448273 | 19 |
GTGCGAG | 55 | 6.002665E-11 | 40.909092 | 1 |
TACGGCT | 475 | 0.0 | 40.73684 | 7 |
CTCGTAA | 100 | 0.0 | 40.5 | 33 |
CTACGGG | 100 | 0.0 | 40.5 | 3 |
CGTAACC | 100 | 0.0 | 40.5 | 35 |
ATAGCGG | 80 | 0.0 | 39.375 | 2 |