##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375053
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431616331558473	31.0	31.0	33.0	28.0	34.0
2	31.340007945543697	31.0	31.0	34.0	28.0	34.0
3	31.275657040471614	31.0	31.0	34.0	28.0	34.0
4	35.09068851602307	37.0	35.0	37.0	32.0	37.0
5	35.3634179702602	37.0	35.0	37.0	33.0	37.0
6	35.31570204744396	37.0	35.0	37.0	33.0	37.0
7	35.72379637011302	37.0	35.0	37.0	35.0	37.0
8	35.404678805395505	37.0	35.0	37.0	33.0	37.0
9	37.3550244898721	39.0	37.0	39.0	34.0	39.0
10	36.72310046846712	38.0	35.0	39.0	32.0	39.0
11	36.82837358986597	39.0	37.0	39.0	33.0	39.0
12	36.99355824376821	39.0	37.0	39.0	33.0	39.0
13	37.065894686884256	39.0	37.0	39.0	33.0	39.0
14	38.124867685367136	40.0	37.0	41.0	33.0	41.0
15	38.23132197316113	40.0	38.0	41.0	33.0	41.0
16	38.26181899624853	40.0	37.0	41.0	34.0	41.0
17	38.12046030827643	40.0	37.0	41.0	33.0	41.0
18	38.11026441596255	40.0	37.0	41.0	34.0	41.0
19	37.99180649134922	40.0	37.0	41.0	34.0	41.0
20	38.05529085222622	40.0	37.0	41.0	34.0	41.0
21	38.00944666487136	40.0	37.0	41.0	34.0	41.0
22	38.1807291236172	40.0	37.0	41.0	34.0	41.0
23	38.24410683290095	40.0	37.0	41.0	34.0	41.0
24	38.178724073664256	40.0	37.0	41.0	34.0	41.0
25	37.86868789211125	40.0	37.0	41.0	33.0	41.0
26	37.95200678304133	40.0	37.0	41.0	34.0	41.0
27	38.03889050347551	40.0	37.0	41.0	34.0	41.0
28	37.945458375216305	40.0	37.0	41.0	33.0	41.0
29	37.96198937216873	40.0	37.0	41.0	34.0	41.0
30	37.719839596003766	40.0	36.0	41.0	33.0	41.0
31	37.74129523027412	40.0	37.0	41.0	33.0	41.0
32	37.702127432656184	40.0	37.0	41.0	33.0	41.0
33	37.698005348577404	40.0	37.0	41.0	33.0	41.0
34	37.68283949201846	40.0	37.0	41.0	33.0	41.0
35	37.66751099177983	40.0	37.0	41.0	33.0	41.0
36	37.574686777602096	40.0	36.0	41.0	33.0	41.0
37	37.58575721298057	40.0	37.0	41.0	33.0	41.0
38	37.43967919200753	40.0	36.0	41.0	33.0	41.0
39	37.413698863893906	40.0	36.0	41.0	33.0	41.0
40	37.28078698210653	40.0	36.0	41.0	33.0	41.0
41	37.18589905959958	40.0	36.0	41.0	32.0	41.0
42	37.214404364183196	40.0	36.0	41.0	32.0	41.0
43	37.15820430712459	40.0	35.0	41.0	32.0	41.0
44	37.11860457055403	39.0	35.0	41.0	32.0	41.0
45	37.07624522400834	39.0	35.0	41.0	32.0	41.0
46	36.96479164278115	39.0	35.0	41.0	32.0	41.0
47	36.899944807800495	39.0	35.0	41.0	32.0	41.0
48	36.81894558902342	39.0	35.0	41.0	31.0	41.0
49	36.82900550055592	39.0	35.0	41.0	32.0	41.0
50	36.700863611276276	39.0	35.0	41.0	31.0	41.0
51	35.45908178310799	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	8.0
10	15.0
11	11.0
12	8.0
13	6.0
14	15.0
15	19.0
16	17.0
17	46.0
18	81.0
19	171.0
20	341.0
21	580.0
22	879.0
23	1140.0
24	1408.0
25	1922.0
26	2325.0
27	2763.0
28	3132.0
29	3730.0
30	4879.0
31	6490.0
32	8784.0
33	12565.0
34	22059.0
35	26278.0
36	29274.0
37	45317.0
38	85972.0
39	114800.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.658629580352645	20.11235745347991	25.019130629537695	11.209882336629756
2	30.213863107347493	27.954715733509666	25.351083713501826	16.480337445641016
3	31.329972030619675	26.951390870090357	26.69276075647975	15.025876342810216
4	27.83473269111299	24.85995312662477	29.341986332598324	17.963327849663916
5	28.879118417930265	29.025231100671107	22.7698485280747	19.32580195332393
6	25.680903765601126	37.125152978379056	22.958088590145927	14.23585466587389
7	79.58901808544393	5.461894718879732	10.810205490957278	4.138881704719067
8	79.80605407769036	7.769835196625544	7.796764723919019	4.627346001765084
9	74.05486691214308	7.278704609748489	9.155772650798687	9.51065582730974
10	38.50309156305909	33.150248098268776	15.728443713288524	12.618216625383614
11	27.96778055368175	22.97541947404767	29.79312257200982	19.26367740026076
12	24.935409128843123	21.238331649126923	32.24157652385129	21.584682698178657
13	23.322570410048712	23.821433237435777	34.2983524995134	18.55764385300211
14	19.735877329337452	28.754869311803933	29.921104483899608	21.588148874959007
15	17.864408496932434	26.16403548298507	35.9173236849192	20.054232335163295
16	21.291924074730773	25.262829520094492	30.90416554460303	22.541080860571704
17	21.20526965522206	25.794754341386415	29.86884520321128	23.13113080018024
18	21.46469965578198	25.186840259909932	30.81191191644914	22.536548167858943
19	22.18593105507755	26.26135506181793	28.861520905045417	22.691192978059103
20	24.136588695464376	26.38773719980909	30.645802059975523	18.82987204475101
21	22.918094242680368	28.772733453671883	28.750603248074274	19.55856905557348
22	21.26312814455557	24.371755458561857	29.53662549026404	24.82849090661853
23	20.7709310417461	27.78194015245845	29.64860966316761	21.798519142627843
24	22.225658773560003	25.205770917710296	29.506229786190218	23.062340522539483
25	20.58962333323557	29.986428584760024	27.365199051867332	22.058749030137072
26	20.332859622506685	28.27200422340309	28.899115591663044	22.496020562427177
27	22.978619021844914	27.693685959051123	27.893391067395807	21.43430395170816
28	18.831471818649632	27.4558529061226	30.927628895116154	22.785046380111613
29	22.93409198166659	25.388145142153242	28.862587420977835	22.81517545520233
30	22.730920696541556	26.000058658376286	29.634744956046212	21.63427568903595
31	23.054075024063266	26.077381063476363	25.608913940163124	25.259629972297247
32	25.06552407259774	27.797937891444676	26.05018490719978	21.0863531287578
33	22.261653686279008	25.8654110219089	27.542773954614415	24.330161337197676
34	21.37484568847603	25.964863632606594	28.260005919163422	24.400284759753955
35	22.19179689270583	25.175641842619576	28.946842179638608	23.68571908503598
36	21.363647271185673	29.261731008684105	26.68689491885147	22.68772680127875
37	23.97421164475421	26.242424404017566	28.2080132674582	21.57535068377003
38	20.7567997056416	26.25122316045999	28.037637347254922	24.954339786643487
39	23.490813298387163	25.2633627780607	27.586501107843425	23.659322815708713
40	21.012763529421175	24.025671038493225	32.365292372011425	22.596273060074175
41	18.971185405795982	27.799537665343298	27.868861200950267	25.360415727910457
42	21.59348145462108	25.94140028209346	30.13040823563603	22.334710027649425
43	23.303639752248348	25.943000055992087	27.292942597446228	23.460417594313338
44	22.14220390184854	24.956739447491422	28.856454954366452	24.04460169629359
45	21.368713221864645	23.10873396559953	29.053227143896997	26.46932566863883
46	24.464808973665058	25.52999176116442	27.424657315099466	22.580541950071055
47	19.902253814794175	24.673846096418373	32.35729350251831	23.06660658626914
48	21.051957989937424	24.721039426427733	28.961240144726215	25.265762438908634
49	20.921576417199702	23.769973843696757	31.85096506360434	23.457484675499195
50	21.189005287252733	24.097927492914334	29.94110165763239	24.771965562200542
51	20.80372640666786	23.224184315283438	27.564637531228918	28.407451746819785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	232.0
2	366.0
3	888.5
4	1411.0
5	1030.5
6	650.0
7	631.5
8	613.0
9	574.5
10	536.0
11	555.0
12	574.0
13	584.5
14	595.0
15	575.5
16	556.0
17	568.0
18	580.0
19	666.0
20	752.0
21	914.0
22	1076.0
23	1370.0
24	1664.0
25	2138.5
26	3302.0
27	3991.0
28	4468.5
29	4946.0
30	5964.0
31	6982.0
32	7810.0
33	8638.0
34	10184.0
35	11730.0
36	12239.0
37	12748.0
38	13812.5
39	14877.0
40	16515.5
41	18154.0
42	19447.0
43	20740.0
44	23693.0
45	26646.0
46	35800.0
47	44954.0
48	41169.0
49	37384.0
50	36573.5
51	35763.0
52	30568.5
53	25374.0
54	22677.5
55	19981.0
56	18063.5
57	16146.0
58	15275.5
59	14405.0
60	13127.5
61	11850.0
62	10586.0
63	9322.0
64	8020.5
65	6719.0
66	5398.0
67	4077.0
68	3338.0
69	2599.0
70	2191.0
71	1783.0
72	1471.0
73	1159.0
74	998.5
75	722.0
76	606.0
77	438.0
78	270.0
79	209.5
80	149.0
81	127.0
82	105.0
83	60.5
84	16.0
85	12.0
86	8.0
87	7.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	375053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.057875241182906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00673160564878	23.803895499448792
2	9.414430419122626	6.2244213252430605
3	3.46812911494485	3.439469388064828
4	1.8916442947839933	2.5013496439065865
5	1.3170709835456402	2.176978412891692
6	1.080882559114243	2.1439008473741485
7	0.9780755480407433	2.263316961050774
8	0.8924138356408962	2.36010441936978
9	0.8148711456417329	2.4244117803237772
>10	8.057413420198072	45.44261461837953
>50	0.048455921640259685	1.0852532478198622
>100	0.020189967350108202	1.0897918222879897
>500	0.006460789552034624	1.5025351239188647
>1k	0.003230394776017312	3.5419569099203225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	4092	1.0910457988604276	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	3830	1.0211890052872528	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	3550	0.9465328900182108	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1795	0.4785990246711798	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	833	0.2221019429253999	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT	814	0.21703599224642917	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	764	0.2037045430912431	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	704	0.1877068041050198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	699	0.18637365918950122	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	647	0.1725089520681077	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	602	0.16051064782844027	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	565	0.15064537545360254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6662898310372135E-4	0.0	0.0	0.18024119257811563	0.0
2	2.6662898310372135E-4	0.0	0.0	1.0875796220800793	0.0
3	2.6662898310372135E-4	0.0	0.0	1.407534401804545	0.0
4	2.6662898310372135E-4	0.0	0.0	1.9733211039506418	0.0
5	2.6662898310372135E-4	0.0	0.0	4.033029998426889	0.0
6	2.6662898310372135E-4	0.0	0.0	4.711334131442756	0.0
7	2.6662898310372135E-4	0.0	0.0	5.585343938056755	0.0
8	2.6662898310372135E-4	0.0	0.0	6.844099367289423	0.0
9	2.6662898310372135E-4	0.0	0.0	7.274438546018829	0.0
10	2.6662898310372135E-4	0.0	0.0	9.104046628076565	0.0
11	2.6662898310372135E-4	0.0	0.0	10.35107038205267	0.0
12	2.6662898310372135E-4	0.0	0.0	12.499566727902456	0.0
13	2.6662898310372135E-4	0.0	0.0	12.959501723756375	0.0
14	2.6662898310372135E-4	0.0	0.0	13.15174122057416	0.0
15	2.6662898310372135E-4	0.0	0.0	13.632739906093272	0.0
16	2.6662898310372135E-4	0.0	0.0	14.300645508768094	0.0
17	2.6662898310372135E-4	0.0	0.0	15.134927596899638	0.0
18	2.6662898310372135E-4	0.0	0.0	16.008137516564325	0.0
19	2.6662898310372135E-4	0.0	0.0	16.762697538747858	0.0
20	2.6662898310372135E-4	0.0	0.0	17.335949852420857	0.0
21	2.6662898310372135E-4	0.0	0.0	17.92573316304629	0.0
22	5.332579662074427E-4	0.0	0.0	18.63443300013598	0.0
23	5.332579662074427E-4	0.0	0.0	19.275942333483535	0.0
24	5.332579662074427E-4	0.0	0.0	19.82306500681237	0.0
25	5.332579662074427E-4	0.0	0.0	20.253937443507983	0.0
26	5.332579662074427E-4	0.0	0.0	20.69867458732499	0.0
27	5.332579662074427E-4	0.0	0.0	21.131680055885436	0.0
28	5.332579662074427E-4	0.0	0.0	21.571084620040367	0.0
29	5.332579662074427E-4	0.0	0.0	22.035552308607052	0.0
30	5.332579662074427E-4	0.0	0.0	22.545346924301366	0.0
31	7.99886949311164E-4	0.0	0.0	23.048475815418087	0.0
32	7.99886949311164E-4	0.0	0.0	23.50227834466062	0.0
33	7.99886949311164E-4	0.0	0.0	23.964879630345578	0.0
34	7.99886949311164E-4	0.0	0.0	24.41281632195983	0.0
35	7.99886949311164E-4	0.0	0.0	24.89568141036067	0.0
36	7.99886949311164E-4	0.0	0.0	25.368414597403568	0.0
37	7.99886949311164E-4	0.0	0.0	25.791554793589174	0.0
38	7.99886949311164E-4	0.0	0.0	26.21549487672409	0.0
39	7.99886949311164E-4	0.0	0.0	26.666631116135587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	20	7.0272037E-4	45.0	1
CGTTCTG	20	7.0272037E-4	45.0	1
ACGGGTG	35	1.2090095E-7	45.0	5
TCTACGG	20	7.0272037E-4	45.0	2
CATAGCG	20	7.0272037E-4	45.0	1
GGGCGTA	20	7.0272037E-4	45.0	7
GTATACG	20	7.0272037E-4	45.0	1
TATAGCG	40	6.7939254E-9	45.0	1
TAGGACG	20	7.0272037E-4	45.0	1
CGGTAGT	25	3.8857637E-5	45.0	12
CGCCGGT	20	7.0272037E-4	45.0	28
TCGAACG	25	3.8857637E-5	45.0	1
TGCGTAG	20	7.0272037E-4	45.0	1
CGATGAA	145	0.0	43.448273	19
GTGCGAG	55	6.002665E-11	40.909092	1
TACGGCT	475	0.0	40.73684	7
CTCGTAA	100	0.0	40.5	33
CTACGGG	100	0.0	40.5	3
CGTAACC	100	0.0	40.5	35
ATAGCGG	80	0.0	39.375	2
>>END_MODULE