SRR3550957_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221304
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	7326	3.310378483895456	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	7316	3.3058598127462675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	6669	3.013501789393775	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	2202	0.995011387051296	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	1443	0.6520442468278929	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGGTAT	1376	0.6217691501283302	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	1335	0.6032425984166576	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTC	1172	0.5295882586848859	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTA	1166	0.5268770559953729	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.4279181578281459	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	930	0.42023641687452556	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	537	0.24265264071141956	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATG	369	0.16673896540505367	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT	355	0.16041282579618987	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGT	321	0.14504934388894913	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCTGGTA	306	0.13827133716516649	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTAT	298	0.1346564002458157	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	285	0.12878212775187073	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	274	0.12381158948776344	TruSeq Adapter, Index 21 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGC	35	1.20599E-7	45.000004	4
CGAATGC	70	0.0	45.000004	45
CGCCGAC	30	2.1575343E-6	45.000004	42
TTGTGCG	20	7.020931E-4	45.0	1
ATAGGGC	40	6.7720975E-9	45.0	4
TACGAAT	25	3.880568E-5	45.0	12
CGAATAT	25	3.880568E-5	45.0	14
GCGATAC	25	3.880568E-5	45.0	33
CGTAAGG	20	7.020931E-4	45.0	2
CGTAAGC	20	7.020931E-4	45.0	43
CATCGAA	75	0.0	45.0	42
GCTACGA	25	3.880568E-5	45.0	10
AGCTACG	25	3.880568E-5	45.0	9
CCGCCGA	25	3.880568E-5	45.0	41
GTAGTTC	20	7.020931E-4	45.0	36
GATGAAT	640	0.0	43.945312	20
ATGAATG	630	0.0	43.571426	21
CGATGAA	590	0.0	43.474575	19
TACGGCT	760	0.0	43.223682	7
CCGATGA	590	0.0	43.09322	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content