##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221304
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23407168419911	31.0	31.0	33.0	28.0	34.0
2	31.14254148140115	31.0	31.0	34.0	28.0	34.0
3	31.085502295484943	31.0	31.0	34.0	28.0	34.0
4	35.027292773741095	35.0	35.0	37.0	32.0	37.0
5	35.20568087336876	35.0	35.0	37.0	33.0	37.0
6	35.08553392618299	36.0	35.0	37.0	32.0	37.0
7	35.38541101832773	37.0	35.0	37.0	33.0	37.0
8	34.831155333839426	37.0	35.0	37.0	32.0	37.0
9	36.53690398727542	39.0	35.0	39.0	32.0	39.0
10	36.254744604706644	37.0	35.0	39.0	32.0	39.0
11	36.51646603766764	38.0	35.0	39.0	32.0	39.0
12	36.60412373929075	39.0	35.0	39.0	32.0	39.0
13	36.52656526768608	39.0	35.0	39.0	32.0	39.0
14	37.53467628239887	39.0	36.0	41.0	32.0	41.0
15	37.499584282254276	39.0	36.0	41.0	32.0	41.0
16	37.734451252575646	39.0	36.0	41.0	33.0	41.0
17	37.37856975020786	39.0	36.0	41.0	32.0	41.0
18	37.35804594584825	39.0	36.0	40.0	32.0	41.0
19	37.27368687416405	39.0	36.0	40.0	32.0	41.0
20	36.9404348769114	39.0	35.0	40.0	31.0	41.0
21	37.205427827784405	39.0	35.0	40.0	32.0	41.0
22	37.31358674041138	39.0	35.0	41.0	32.0	41.0
23	37.30996728482088	39.0	35.0	41.0	32.0	41.0
24	37.23557640169179	39.0	35.0	41.0	32.0	41.0
25	37.186670823844125	39.0	35.0	40.0	32.0	41.0
26	37.07229873838701	39.0	35.0	41.0	32.0	41.0
27	36.99900137367603	39.0	35.0	41.0	31.0	41.0
28	36.79138199038427	39.0	35.0	41.0	30.0	41.0
29	36.84318855510971	39.0	35.0	41.0	31.0	41.0
30	36.5181063152948	39.0	35.0	40.0	30.0	41.0
31	36.70468676571594	39.0	35.0	40.0	30.0	41.0
32	36.311770234609405	39.0	35.0	40.0	30.0	41.0
33	36.29439594404078	39.0	35.0	41.0	30.0	41.0
34	36.029158984925715	39.0	35.0	41.0	29.0	41.0
35	35.67402306329755	39.0	35.0	41.0	27.0	41.0
36	35.85054947041174	39.0	35.0	40.0	29.0	41.0
37	35.65995644001012	39.0	35.0	40.0	28.0	41.0
38	35.621466399161335	38.0	35.0	40.0	27.0	41.0
39	35.594404077648846	39.0	35.0	40.0	27.0	41.0
40	35.309899504753645	38.0	35.0	40.0	26.0	41.0
41	35.245074648447385	38.0	34.0	40.0	25.0	41.0
42	35.11382080757691	38.0	34.0	40.0	24.0	41.0
43	35.20428912265481	38.0	35.0	40.0	26.0	41.0
44	35.370119835158874	38.0	35.0	40.0	26.0	41.0
45	35.37452102085819	38.0	35.0	40.0	26.0	41.0
46	35.23421176300474	38.0	35.0	40.0	26.0	41.0
47	34.97170859993493	38.0	34.0	40.0	26.0	41.0
48	34.96510230271482	38.0	34.0	40.0	26.0	41.0
49	34.925726602320786	38.0	34.0	40.0	25.0	41.0
50	34.7806456277338	37.0	34.0	40.0	24.0	41.0
51	33.303749593319594	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	10.0
10	4.0
11	6.0
12	7.0
13	3.0
14	12.0
15	16.0
16	25.0
17	61.0
18	113.0
19	312.0
20	677.0
21	1290.0
22	1696.0
23	1877.0
24	2061.0
25	2233.0
26	2707.0
27	2778.0
28	2869.0
29	3419.0
30	4389.0
31	5614.0
32	7477.0
33	10032.0
34	16230.0
35	18016.0
36	18663.0
37	27375.0
38	45752.0
39	45572.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.35695694610129	16.50083143549145	20.132939305209124	12.009272313198133
2	28.45000542240538	37.30253407078046	19.487673065105014	14.759787441709143
3	26.29098434732314	37.141669377869356	23.38999746954416	13.177348805263348
4	24.920019520659366	19.963941004229476	38.3702960633337	16.745743411777465
5	34.307106965983444	23.484889563677115	22.46231428261577	19.745689187723674
6	22.056085746303726	40.92198966128041	23.742905686295774	13.279018906120088
7	66.39328706214077	5.169811661786502	23.495282507320248	4.941618768752485
8	63.30341973032571	16.86458446300112	12.99976502910024	6.832230777572931
9	58.66771499837328	6.764902577450023	12.75440118569931	21.812981238477388
10	41.57810071214257	20.526515562303437	21.784513610237504	16.110870115316487
11	34.46209738640061	20.94810758052272	24.76141416332285	19.828380869753822
12	25.30546216968514	18.028594151032067	30.368633192350792	26.297310486932002
13	25.468134331055925	20.85321548638976	36.252394895709074	17.426255286845244
14	19.860011567798143	31.41696489896251	25.96925496150092	22.753768571738423
15	16.420850956150815	22.14148863102339	39.82847124317681	21.60918916964899
16	17.85417344467339	23.864909807323862	27.78847196616419	30.492444781838557
17	18.248201568882624	26.583342370675634	31.094331778910455	24.074124281531287
18	18.696905613997036	24.13557820916025	30.31621660702021	26.85129956982251
19	20.04075841376568	24.864439865524346	26.122437913458413	28.97236380725156
20	24.822416223836893	24.554910891804937	32.62661316560026	17.996059718757905
21	22.150977840436685	31.607652821458267	27.526389039511262	18.71498029859379
22	18.65262263673499	22.051115208039622	31.360933376712573	27.93532877851281
23	22.12205834508188	28.32167516176843	28.301793008712	21.254473484437696
24	23.554477099374616	23.957542565882225	25.904186096952607	26.58379423779055
25	18.129360517658967	34.62838448469074	24.328525467230598	22.913729530419694
26	20.230542602031594	21.89070238224343	31.133192350793475	26.745562664931498
27	25.609568738025523	25.576130571521528	25.661533456241187	23.152767234211762
28	17.62372121606478	26.786230705274193	30.780284134041864	24.809763944619167
29	26.763637349528253	20.07690778295919	29.418356649676465	23.741098217836097
30	21.355691718179518	26.522340310161585	31.504627119256767	20.617340852402126
31	24.92137512200412	21.64940534287677	23.20518381954235	30.224035715576765
32	27.761811806383978	25.236326501102557	28.362795069226042	18.639066623287423
33	27.51599609586813	22.31319813469255	23.52917254093916	26.64163322850016
34	30.37767053464917	21.234591331381267	26.367801756859343	22.01993637711022
35	22.549072768680187	21.35478798394968	32.23122944004627	23.864909807323862
36	26.25528684524455	27.480750460904456	22.96614611575028	23.297816578100715
37	21.08547518345805	21.689169648989626	37.26005856197809	19.96529660557423
38	22.941293424429745	23.22868090951813	24.44149224596031	29.388533420091818
39	27.668727180710697	22.303708925279253	29.507826338430394	20.519737555579656
40	25.7193724469508	20.14242851462242	32.4070057477497	21.73119329067708
41	23.472689151574304	27.14727253009435	24.771807106965984	24.60823121136536
42	20.89659472942197	21.271644434804614	35.04636156599067	22.78539926978274
43	25.382279579221347	24.989607056356867	25.50473556736435	24.12337779705744
44	23.91235585439034	20.684669052525035	29.641127137331452	25.76184795575317
45	21.407656436395182	18.559538011061708	28.870241839279903	31.162563713263204
46	29.126450493438888	23.878917687886346	26.109785634240684	20.884846184434082
47	19.40724071864946	22.21966164190435	37.68390991577197	20.68918772367422
48	23.382767595705456	23.459133138126738	26.07228066370242	27.085818602465388
49	21.162292592994252	19.431641542855075	36.45980190145682	22.94626396269385
50	23.67060694790876	20.456928026605937	30.11603947511116	25.756425550374146
51	22.233217655351915	19.592506235766187	26.20287025991396	31.971405848967937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	175.5
2	268.0
3	980.5
4	1693.0
5	1172.5
6	652.0
7	649.0
8	646.0
9	610.5
10	575.0
11	555.5
12	536.0
13	506.5
14	477.0
15	496.5
16	516.0
17	505.5
18	495.0
19	485.5
20	476.0
21	551.0
22	626.0
23	602.0
24	578.0
25	700.5
26	1051.0
27	1279.0
28	1484.0
29	1689.0
30	2037.5
31	2386.0
32	2711.0
33	3036.0
34	3322.0
35	3608.0
36	4117.0
37	4626.0
38	5060.5
39	5495.0
40	6241.5
41	6988.0
42	8017.5
43	9047.0
44	10312.0
45	11577.0
46	27043.0
47	42509.0
48	32314.0
49	22119.0
50	22172.0
51	22225.0
52	18663.0
53	15101.0
54	13699.5
55	12298.0
56	11327.5
57	10357.0
58	9811.5
59	9266.0
60	8798.5
61	8331.0
62	7436.5
63	6542.0
64	5542.0
65	4542.0
66	3828.0
67	3114.0
68	2686.5
69	2259.0
70	1941.0
71	1623.0
72	1424.0
73	1225.0
74	944.5
75	518.0
76	372.0
77	337.5
78	303.0
79	225.5
80	148.0
81	119.0
82	90.0
83	56.0
84	22.0
85	15.0
86	8.0
87	7.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	221304.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.058525828724285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54640784275472	27.625799804793406
2	8.725552358191484	6.467122148718504
3	3.5543578988440716	3.9515779199652967
4	2.1387114080866216	3.170299678270614
5	1.6765839145490906	3.1065864150670572
6	1.3449251329073795	2.990456566532914
7	1.1778764083304882	3.0555254310812274
8	1.0876457103838464	3.224523732060876
9	0.9218163195629908	3.074503849907819
>10	4.741988977222846	25.89514875465423
>50	0.04755401648539238	1.0989408234826301
>100	0.021948007608642635	1.690434876911398
>500	0.003658001268107106	1.0908072154140909
>1k	0.007316002536214212	3.9285326971044356
>5k	0.003658001268107106	9.629740086035499
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	7326	3.310378483895456	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	7316	3.3058598127462675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	6669	3.013501789393775	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	2202	0.995011387051296	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	1443	0.6520442468278929	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGGTAT	1376	0.6217691501283302	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	1335	0.6032425984166576	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTC	1172	0.5295882586848859	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTA	1166	0.5268770559953729	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.4279181578281459	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	930	0.42023641687452556	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	537	0.24265264071141956	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATG	369	0.16673896540505367	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTT	355	0.16041282579618987	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGT	321	0.14504934388894913	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCTGGTA	306	0.13827133716516649	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTAT	298	0.1346564002458157	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	285	0.12878212775187073	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	274	0.12381158948776344	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2598235910783357	0.0
2	0.0	0.0	0.0	2.0153273325380474	0.0
3	0.0	0.0	0.0	2.4766836568701875	0.0
4	0.0	0.0	0.0	3.5769800816975743	0.0
5	0.0	0.0	0.0	8.315710515851498	0.0
6	0.0	0.0	0.0	9.199562592632759	0.0
7	4.518671149188447E-4	0.0	0.0	10.632433214040415	0.0
8	4.518671149188447E-4	0.0	0.0	12.290333658677657	0.0
9	4.518671149188447E-4	0.0	0.0	12.73903770379207	0.0
10	4.518671149188447E-4	0.0	0.0	16.713208979503307	0.0
11	4.518671149188447E-4	0.0	0.0	18.067454722915084	0.0
12	4.518671149188447E-4	0.0	0.0	22.61504536745834	0.0
13	4.518671149188447E-4	0.0	0.0	23.29555724252612	0.0
14	4.518671149188447E-4	0.0	0.0	23.64259118678379	0.0
15	4.518671149188447E-4	0.0	0.0	24.56485196833315	0.0
16	4.518671149188447E-4	0.0	0.0	25.402161732277772	0.0
17	4.518671149188447E-4	0.0	0.0	26.231337888153853	0.0
18	4.518671149188447E-4	0.0	0.0	27.082655532660954	0.0
19	4.518671149188447E-4	0.0	0.0	28.25751003144995	0.0
20	4.518671149188447E-4	0.0	0.0	28.878375447348443	0.0
21	4.518671149188447E-4	0.0	0.0	29.42829772620468	0.0
22	4.518671149188447E-4	0.0	0.0	30.14767017315548	0.0
23	4.518671149188447E-4	0.0	0.0	30.707533528539926	0.0
24	4.518671149188447E-4	0.0	0.0	31.149007699815638	0.0
25	4.518671149188447E-4	0.0	0.0	31.545747026714384	0.0
26	4.518671149188447E-4	0.0	0.0	31.9298340743954	0.0
27	4.518671149188447E-4	0.0	0.0	32.421917362542025	0.0
28	4.518671149188447E-4	0.0	0.0	32.789737194085966	0.0
29	4.518671149188447E-4	0.0	0.0	33.16523876658352	0.0
30	4.518671149188447E-4	0.0	0.0	33.61394281169794	0.0
31	4.518671149188447E-4	0.0	0.0	34.0039041318729	0.0
32	4.518671149188447E-4	0.0	0.0	34.38708744532408	0.0
33	4.518671149188447E-4	0.0	0.0	34.79331598163612	0.0
34	4.518671149188447E-4	0.0	0.0	35.15209847088168	0.0
35	9.037342298376894E-4	0.0	0.0	35.51765896685103	0.0
36	9.037342298376894E-4	0.0	0.0	35.88547879839497	0.0
37	9.037342298376894E-4	0.0	0.0	36.2700177131909	0.0
38	9.037342298376894E-4	0.0	0.0	36.6464230199183	0.0
39	9.037342298376894E-4	0.0	0.0	37.03909554278278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGC	35	1.20599E-7	45.000004	4
CGAATGC	70	0.0	45.000004	45
CGCCGAC	30	2.1575343E-6	45.000004	42
TTGTGCG	20	7.020931E-4	45.0	1
ATAGGGC	40	6.7720975E-9	45.0	4
TACGAAT	25	3.880568E-5	45.0	12
CGAATAT	25	3.880568E-5	45.0	14
GCGATAC	25	3.880568E-5	45.0	33
CGTAAGG	20	7.020931E-4	45.0	2
CGTAAGC	20	7.020931E-4	45.0	43
CATCGAA	75	0.0	45.0	42
GCTACGA	25	3.880568E-5	45.0	10
AGCTACG	25	3.880568E-5	45.0	9
CCGCCGA	25	3.880568E-5	45.0	41
GTAGTTC	20	7.020931E-4	45.0	36
GATGAAT	640	0.0	43.945312	20
ATGAATG	630	0.0	43.571426	21
CGATGAA	590	0.0	43.474575	19
TACGGCT	760	0.0	43.223682	7
CCGATGA	590	0.0	43.09322	18
>>END_MODULE