SRR3550956_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489989
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	32951	6.72484484345567	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	21167	4.319892895554798	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	18838	3.844576102728837	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15062	3.073946557983955	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10293	2.1006594025580165	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	10197	2.081067125996706	No Hit
CGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTG	7727	1.5769741769713197	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	7238	1.477176018237144	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	5248	1.0710444520183107	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	4561	0.9308372228764319	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT	4414	0.9008365493919251	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	4319	0.8814483590447949	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	4187	0.8545089787729929	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTT	3195	0.6520554543061171	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	3001	0.6124627287551353	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	2432	0.4963376728865342	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	2191	0.4471528952690775	No Hit
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1819	0.37123282359399906	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	1578	0.3220480459765423	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCC	1351	0.2757204753576101	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	1170	0.23878087059097247	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1072	0.21878042160130123	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATG	997	0.2034739555377774	No Hit
AAAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	966	0.1971472828981875	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGT	921	0.1879634032600732	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACATGTTCGT	881	0.17979995469286048	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	842	0.17184059233982804	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTAT	792	0.16163628163081212	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCACATGT	708	0.1444930396396654	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	589	0.12020678015220751	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCGCACAT	521	0.10632891758794585	Illumina DpnII expression PCR Primer 2 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGG	20	7.029319E-4	45.000004	2
TAGACGG	20	7.029319E-4	45.000004	2
CGCATGG	20	7.029319E-4	45.000004	2
CGCATCG	20	7.029319E-4	45.000004	21
CCTACAT	20	7.029319E-4	45.000004	27
CACGATC	20	7.029319E-4	45.000004	39
ACGATCG	20	7.029319E-4	45.000004	40
CCCCGTA	20	7.029319E-4	45.000004	40
GCCGACT	25	3.8875172E-5	45.000004	43
CTTGCGG	20	7.029319E-4	45.000004	2
CGAATAT	20	7.029319E-4	45.000004	14
CTCGAAT	55	1.8189894E-12	45.000004	43
GCGTGAG	25	3.8875172E-5	45.000004	1
GCTACGA	20	7.029319E-4	45.000004	10
CGACTGG	20	7.029319E-4	45.000004	2
GGCGACG	20	7.029319E-4	45.000004	32
CACCGAG	110	0.0	45.000004	16
CCAGTCG	20	7.029319E-4	45.000004	28
ACCCATG	20	7.029319E-4	45.000004	17
ACCCACG	25	3.8875172E-5	45.000004	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content