FastQCFastQC Report
Sat 18 Jun 2016
SRR3550955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128873
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15781.2244612913488473No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC5970.46324676231638895No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC5470.4244488760252341No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT4150.3220224562165853No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC4100.31814266758746984No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC2450.19010964282665882No Hit
ACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC1840.14277622155144987No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC1670.12958494021245723No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC1480.11484174342181838No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC1420.11018599706687979No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTC1310.10165046208282572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATT207.0099643E-445.00000441
GAGCTAG207.0099643E-445.0000041
CCAGCGG207.0099643E-445.0000042
GTGATAG207.0099643E-445.0000041
CGTCTCT207.0099643E-445.00000435
AAGGTAT207.0099643E-445.00000440
TTCAACA207.0099643E-445.00000424
CGGGGTC207.0099643E-445.0000046
CTACGAA207.0099643E-445.00000411
TACGAAT207.0099643E-445.00000412
CGGGCTC207.0099643E-445.0000046
GGAACGT207.0099643E-445.0000048
TAGGGCG406.7338988E-945.0000045
TCCAATC207.0099643E-445.00000412
GCTACGA207.0099643E-445.00000410
GGATTAC207.0099643E-445.0000048
TAAGCGG207.0099643E-445.0000042
AGGGAGC406.7338988E-945.0000046
GTAACGG207.0099643E-445.0000042
TCAACAA207.0099643E-445.00000425