SRR3550954_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417197
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	19078	4.5728996133721	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	18151	4.350702425952248	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	16207	3.8847355086445967	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	4673	1.1200943439190598	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	3788	0.9079643429842497	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	3579	0.857868105475351	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	3436	0.8235917324429467	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	2800	0.6711457656694559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	2526	0.6054693586003734	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	0.5783838330572847	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTT	1179	0.282600306330103	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	905	0.21692389926102057	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGT	714	0.17114217024571124	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	684	0.16395132275639565	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	679	0.16275284817484306	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTAT	673	0.16131467867697993	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACGGTAG	604	0.14477572945155406	No Hit
CGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTG	493	0.11816959374108635	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTACGGT	469	0.11241691574963386	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	460	0.1102596615028392	Illumina Single End PCR Primer 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGT	35	1.209446E-7	45.000004	28
ATAACGG	35	1.209446E-7	45.000004	2
CAGTCGA	70	0.0	45.000004	41
TACCGCG	35	1.209446E-7	45.000004	29
CCCTCGA	20	7.0281135E-4	45.0	41
GTCGAAT	95	0.0	45.0	43
GACACGA	40	6.7975634E-9	45.0	25
GATCCTC	25	3.8865182E-5	45.0	9
ACATGCG	20	7.0281135E-4	45.0	1
CGTCGAA	45	3.8380676E-10	45.0	42
CGAGACA	40	6.7975634E-9	45.0	22
TTGCCGG	20	7.0281135E-4	45.0	2
TCGAATA	20	7.0281135E-4	45.0	44
GCGTAAG	45	3.8380676E-10	45.0	1
CCGATGA	2110	0.0	43.82701	18
ACCGATG	2045	0.0	43.12958	17
CGTTTTT	1550	0.0	43.112904	1
CGATGAA	2205	0.0	43.061222	19
CGGCTAT	115	0.0	43.04348	31
TGAATGA	2185	0.0	42.83753	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content