##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417197
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18919599134222	31.0	31.0	33.0	30.0	34.0
2	31.076251746776702	31.0	31.0	34.0	28.0	34.0
3	31.059933316874282	31.0	30.0	34.0	28.0	34.0
4	35.006574831554396	35.0	35.0	37.0	32.0	37.0
5	35.14507295114778	35.0	35.0	37.0	33.0	37.0
6	35.03402709031944	35.0	35.0	37.0	32.0	37.0
7	35.297252856564164	35.0	35.0	37.0	33.0	37.0
8	34.59805799178805	35.0	35.0	37.0	30.0	37.0
9	36.44120403550361	39.0	35.0	39.0	32.0	39.0
10	36.212983314836876	37.0	35.0	39.0	32.0	39.0
11	36.482891775348335	38.0	35.0	39.0	32.0	39.0
12	36.6490411004873	39.0	35.0	39.0	32.0	39.0
13	36.52642276909949	39.0	35.0	39.0	32.0	39.0
14	37.64331478893664	39.0	36.0	41.0	32.0	41.0
15	37.47118267868657	39.0	36.0	41.0	32.0	41.0
16	37.87823498251426	39.0	37.0	41.0	33.0	41.0
17	37.29335541722496	39.0	36.0	41.0	31.0	41.0
18	37.29968336301555	39.0	36.0	40.0	32.0	41.0
19	37.29645706944201	39.0	36.0	40.0	32.0	41.0
20	36.72731826930683	39.0	35.0	40.0	30.0	41.0
21	37.12238103342066	39.0	35.0	40.0	31.0	41.0
22	37.107014192335996	39.0	35.0	41.0	31.0	41.0
23	37.19535375374224	39.0	35.0	41.0	31.0	41.0
24	37.106110542501504	39.0	35.0	41.0	31.0	41.0
25	37.298921133181686	39.0	35.0	40.0	32.0	41.0
26	37.00082455051211	39.0	35.0	41.0	31.0	41.0
27	36.81845746733558	39.0	35.0	41.0	31.0	41.0
28	36.631145478035556	39.0	35.0	41.0	30.0	41.0
29	36.757088377912595	39.0	35.0	41.0	30.0	41.0
30	36.54806242614401	39.0	35.0	41.0	30.0	41.0
31	36.76846669558985	39.0	35.0	41.0	30.0	41.0
32	36.22527007624695	39.0	35.0	41.0	29.0	41.0
33	36.070928122685444	39.0	35.0	41.0	27.0	41.0
34	35.53774835389516	39.0	35.0	41.0	25.0	41.0
35	35.41440134996177	39.0	35.0	41.0	24.0	41.0
36	35.40700196789526	39.0	35.0	41.0	24.0	41.0
37	35.286061500921626	39.0	35.0	41.0	23.0	41.0
38	35.46584946679866	39.0	35.0	41.0	24.0	41.0
39	35.04838481580644	39.0	35.0	40.0	22.0	41.0
40	34.904234690086454	38.0	35.0	40.0	22.0	41.0
41	34.7290872177892	38.0	34.0	40.0	22.0	41.0
42	34.77160669899352	38.0	35.0	40.0	22.0	41.0
43	34.823145899898606	38.0	35.0	40.0	21.0	41.0
44	35.07716258745868	39.0	35.0	40.0	22.0	41.0
45	35.216808845701195	39.0	35.0	40.0	23.0	41.0
46	35.03632576456686	39.0	35.0	40.0	22.0	41.0
47	34.932298170888096	38.0	34.0	40.0	23.0	41.0
48	34.88070863405058	38.0	34.0	40.0	23.0	41.0
49	34.992428037593754	38.0	34.0	40.0	23.0	41.0
50	34.674218654496556	38.0	34.0	40.0	21.0	41.0
51	33.45795151930623	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	15.0
11	3.0
12	5.0
13	3.0
14	8.0
15	13.0
16	28.0
17	66.0
18	217.0
19	674.0
20	1674.0
21	2742.0
22	3632.0
23	4320.0
24	5043.0
25	5986.0
26	6789.0
27	7019.0
28	6586.0
29	6717.0
30	7855.0
31	10079.0
32	13430.0
33	17757.0
34	25619.0
35	30121.0
36	33264.0
37	46720.0
38	82794.0
39	97998.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.754228817561014	15.113723253043526	18.22280601250728	11.909241916888185
2	24.569208311660915	44.57007121335963	18.372375640285046	12.4883448346944
3	22.666030676155387	43.72418785369981	22.323506640747656	11.286274829397144
4	21.5612768068802	17.968250011385507	46.88983861341285	13.58063456832144
5	39.498606174061656	21.224745144380233	21.630548637693945	17.64610004386417
6	19.173915440427425	45.35890718293756	23.537082002027816	11.9300953746072
7	61.549340000047934	4.106693001148138	30.30414887930642	4.039818119497504
8	58.25473337535985	23.16411671224865	12.99002629453232	5.591123617859188
9	54.016927254989845	6.292950332816391	11.805933407958351	27.88418900423541
10	39.692998751189485	27.10949503471981	19.809106968650305	13.388399245440402
11	32.77923858512885	18.892273913762565	29.868143826537587	18.460343674571007
12	20.80000575267799	15.88458210389816	33.58844862259316	29.726963520830687
13	25.767203503380898	18.611591166762945	41.001972689161235	14.619232640694923
14	18.332106894344875	34.65149557643032	26.73221523644705	20.284182292777754
15	12.916919345057613	21.87359928283281	41.93486530344178	23.2746160686678
16	15.000347557628652	24.479802107877095	27.449861815880748	33.069988518613506
17	15.485250373324833	28.987265009096426	34.00024448881464	21.52724012876411
18	15.16981186346019	24.74969858364274	30.515559795492297	29.564929757404773
19	15.705050611581578	25.019115669575765	28.828347279582545	30.447486439260107
20	24.09533146211502	24.747061939563324	36.026625311303775	15.130981287017883
21	18.555502556346283	35.76871358135365	29.164639247166207	16.51114461513386
22	15.96823562969053	20.38293659829769	36.87898043370399	26.7698473383078
23	21.54665541698526	29.65960924934743	31.031862645225157	17.76187268844215
24	20.469226768169474	27.419899951341932	26.79765194859982	25.313221331888773
25	14.846703116273607	40.45115377147966	25.060822585013792	19.64132052723294
26	15.731177357459424	22.092680436340626	36.619630534256	25.556511671943948
27	25.880579198795772	27.530878697593703	25.442656586696454	21.145885516914074
28	14.1515878589731	28.703705923101076	34.809694221195265	22.33501199673056
29	21.293777280277663	20.072291986759254	36.527587686392756	22.106343046570327
30	17.26834085575879	35.62465693665103	29.81636972461451	17.290632482975667
31	21.40787206044147	20.733610260859976	25.377219874543684	32.48129780415488
32	30.85209145799227	28.96425429713061	23.94240610550891	16.24124813936821
33	28.284000124641356	21.009499109533387	23.22859464473618	27.47790612108908
34	22.517899217875488	21.41674077234496	33.961893302204956	22.103466707574597
35	22.714449049250117	29.376289858268397	25.43738329853762	22.471877793943868
36	23.280129051742943	25.73628285917684	28.76530751659288	22.21828057248734
37	20.910984498929764	28.5265713799476	31.97314458157657	18.58929953954607
38	23.36210471312114	23.285162644985462	21.64708758691937	31.70564505497403
39	28.357107074116065	21.771489248484528	30.165125827846317	19.70627784955309
40	23.389429933580537	25.654067502882334	29.556780130250214	21.399722433286914
41	21.16817714413095	25.25161973839697	29.51219687581646	24.06800624165562
42	19.350570593748277	28.122206056131756	32.54265970273036	19.984563647389603
43	29.246614908544405	22.38055403082956	25.009527872923343	23.36330318770269
44	21.803848062186447	22.120005656800025	31.386850816281036	24.68929546473249
45	19.325402627535674	20.318938055642775	28.647377617768104	31.708281699053444
46	31.496151698118634	24.4776448536303	24.718538244522374	19.307665203728693
47	17.727116925577125	24.597732006701868	38.47127376275476	19.203877304966237
48	21.313911653247747	25.571852146587826	26.464236320011892	26.649999880152542
49	20.042090427304128	21.239845924107794	36.43458605886428	22.283477589723798
50	23.94791908858405	20.312705987818706	30.520593388734817	25.218781534862426
51	20.170327207530256	20.023873613664527	29.177343077730665	30.62845610107455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	210.5
2	272.0
3	2316.0
4	4360.0
5	3017.5
6	1675.0
7	1608.5
8	1542.0
9	1470.0
10	1398.0
11	1360.0
12	1322.0
13	1322.5
14	1323.0
15	1247.0
16	1171.0
17	1149.5
18	1128.0
19	1240.5
20	1353.0
21	1459.0
22	1565.0
23	1757.0
24	1949.0
25	2149.0
26	3192.5
27	4036.0
28	4673.0
29	5310.0
30	6117.0
31	6924.0
32	7939.5
33	8955.0
34	9898.5
35	10842.0
36	11137.0
37	11432.0
38	12808.5
39	14185.0
40	15247.0
41	16309.0
42	17799.0
43	19289.0
44	21968.5
45	24648.0
46	61039.5
47	97431.0
48	69278.0
49	41125.0
50	39098.5
51	37072.0
52	30227.0
53	23382.0
54	21070.5
55	18759.0
56	16985.0
57	15211.0
58	13492.0
59	11773.0
60	10960.5
61	10148.0
62	8828.0
63	7508.0
64	6021.0
65	4534.0
66	3534.5
67	2535.0
68	2031.5
69	1528.0
70	1314.0
71	1100.0
72	877.5
73	655.0
74	551.0
75	328.5
76	210.0
77	185.5
78	161.0
79	112.5
80	64.0
81	46.5
82	29.0
83	22.5
84	16.0
85	10.0
86	4.0
87	4.5
88	5.0
89	8.5
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.64165916532079
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03916849683344	25.244421306195473
2	8.520478237424793	5.732860495779562
3	3.219901481625229	3.2496848457224212
4	2.035320612362618	2.7388624933302066
5	1.482038050435931	2.4929109481401044
6	1.2576518839317188	2.538569761671268
7	1.123519805656779	2.6457949257174964
8	0.9556682565540493	2.5720212609686133
9	0.8318514426816127	2.518637643978767
>10	5.440792889386394	27.68461855779723
>50	0.05930941967876956	1.4260151225990638
>100	0.02215171096435971	1.421928430575432
>500	0.004287427928585751	1.0238365487440009
>1k	0.005716570571447668	5.8641626602632435
>5k	0.0	0.0
>10k+	0.0021437139642928754	12.845674998517124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	19078	4.5728996133721	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	18151	4.350702425952248	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	16207	3.8847355086445967	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	4673	1.1200943439190598	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	3788	0.9079643429842497	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCT	3579	0.857868105475351	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCT	3436	0.8235917324429467	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGGTAGTCGTA	2800	0.6711457656694559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTC	2526	0.6054693586003734	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2413	0.5783838330572847	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTT	1179	0.282600306330103	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATG	905	0.21692389926102057	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGT	714	0.17114217024571124	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	684	0.16395132275639565	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	679	0.16275284817484306	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTAT	673	0.16131467867697993	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACGGTAG	604	0.14477572945155406	No Hit
CGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTG	493	0.11816959374108635	No Hit
TAATGATACGGCGACCACCGACTGTCTCTTATACACATCTGACGCTACGGT	469	0.11241691574963386	Illumina DpnII expression PCR Primer 2 (95% over 23bp)
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	460	0.1102596615028392	Illumina Single End PCR Primer 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3969491631051996E-4	0.0	0.0	0.16898491599891657	0.0
2	2.3969491631051996E-4	0.0	0.0	1.6294460410789147	0.0
3	2.3969491631051996E-4	0.0	0.0	1.9348173644585172	0.0
4	2.3969491631051996E-4	0.0	0.0	3.0525147592144717	0.0
5	2.3969491631051996E-4	0.0	0.0	8.404183155679451	0.0
6	2.3969491631051996E-4	0.0	0.0	9.218187091469977	0.0
7	2.3969491631051996E-4	0.0	0.0	10.689674182700259	0.0
8	2.3969491631051996E-4	0.0	0.0	12.096683341443011	0.0
9	2.3969491631051996E-4	0.0	0.0	12.448555478586854	0.0
10	2.3969491631051996E-4	0.0	0.0	17.11182007540802	0.0
11	2.3969491631051996E-4	0.0	0.0	18.018825638727026	0.0
12	2.3969491631051996E-4	0.0	0.0	23.71373715534867	0.0
13	2.3969491631051996E-4	0.0	0.0	24.30242786980731	0.0
14	2.3969491631051996E-4	0.0	0.0	24.633446549232136	0.0
15	2.3969491631051996E-4	0.0	0.0	25.588151400896937	0.0
16	2.3969491631051996E-4	0.0	0.0	26.171089437364124	0.0
17	2.3969491631051996E-4	0.0	0.0	26.79189927060837	0.0
18	2.3969491631051996E-4	0.0	0.0	27.37196096807983	0.0
19	2.3969491631051996E-4	0.0	0.0	28.827867889749925	0.0
20	2.3969491631051996E-4	0.0	0.0	29.361189078540832	0.0
21	2.3969491631051996E-4	0.0	0.0	29.794317792313944	0.0
22	2.3969491631051996E-4	0.0	0.0	30.53018118538724	0.0
23	2.3969491631051996E-4	0.0	0.0	31.016042780748663	0.0
24	2.3969491631051996E-4	0.0	0.0	31.450849358935947	0.0
25	2.3969491631051996E-4	0.0	0.0	31.74879013990992	0.0
26	2.3969491631051996E-4	0.0	0.0	32.11168824320405	0.0
27	2.3969491631051996E-4	0.0	0.0	32.69174994067551	0.0
28	2.3969491631051996E-4	0.0	0.0	33.01006478953588	0.0
29	2.3969491631051996E-4	0.0	0.0	33.3432407232075	0.0
30	2.3969491631051996E-4	0.0	0.0	33.72387625030861	0.0
31	2.3969491631051996E-4	0.0	0.0	34.08893160784953	0.0
32	2.3969491631051996E-4	0.0	0.0	34.500727474071006	0.0
33	2.3969491631051996E-4	0.0	0.0	34.80705757711585	0.0
34	2.3969491631051996E-4	0.0	0.0	35.226044290826636	0.0
35	2.3969491631051996E-4	0.0	0.0	35.56161717366136	0.0
36	2.3969491631051996E-4	0.0	0.0	35.888800734425224	0.0
37	2.3969491631051996E-4	0.0	0.0	36.23803622748965	0.0
38	2.3969491631051996E-4	0.0	0.0	36.55611138143371	0.0
39	2.3969491631051996E-4	0.0	0.0	36.880418603201846	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGGT	35	1.209446E-7	45.000004	28
ATAACGG	35	1.209446E-7	45.000004	2
CAGTCGA	70	0.0	45.000004	41
TACCGCG	35	1.209446E-7	45.000004	29
CCCTCGA	20	7.0281135E-4	45.0	41
GTCGAAT	95	0.0	45.0	43
GACACGA	40	6.7975634E-9	45.0	25
GATCCTC	25	3.8865182E-5	45.0	9
ACATGCG	20	7.0281135E-4	45.0	1
CGTCGAA	45	3.8380676E-10	45.0	42
CGAGACA	40	6.7975634E-9	45.0	22
TTGCCGG	20	7.0281135E-4	45.0	2
TCGAATA	20	7.0281135E-4	45.0	44
GCGTAAG	45	3.8380676E-10	45.0	1
CCGATGA	2110	0.0	43.82701	18
ACCGATG	2045	0.0	43.12958	17
CGTTTTT	1550	0.0	43.112904	1
CGATGAA	2205	0.0	43.061222	19
CGGCTAT	115	0.0	43.04348	31
TGAATGA	2185	0.0	42.83753	22
>>END_MODULE