FastQCFastQC Report
Sat 18 Jun 2016
SRR3550948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583527
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73591.2611241639204356No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC28360.48601007322711715No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC26120.44762281779592034No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG22770.39021330632515716No Hit
CGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTG15060.2580857441043859No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC12740.21832751526493205Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT10350.17736968469325326No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC9660.16554503904703638Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT9540.16348857893465082No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT9170.15714782692146206Illumina Single End Adapter 2 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7890.13521225238934959No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT6980.11961742987042587No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC6760.11584725299771904No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5960.10213751891514873No Hit
CGTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT5890.10093791718292384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG453.8380676E-1045.0000041
GCTACGA502.1827873E-1145.00000410
GACCGAA207.030428E-445.011
GACCCGT207.030428E-445.012
ACGATAG207.030428E-445.01
CGTTTTT47650.043.3945431
ATAGCGG800.042.18752
CTACGAA556.002665E-1140.90909211
CGTAAGG501.0804797E-940.5000042
ACGGGAC1550.039.1935465
TCGTGTA1800.038.75000417
GTTTTTT54700.038.0073172
CCTCGTG1850.037.702715
GTAGGGA3350.037.6119424
TCTACGG301.1392332E-437.4999962
CGAATAT601.546141E-1037.49999614
GCGATTC301.1392332E-437.4999969
GCTAACG301.1392332E-437.4999961
CGATCCC301.1392332E-437.49999643
TGCGTTG301.1392332E-437.4999961