##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550948_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583527
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.382640391961296	31.0	31.0	33.0	28.0	34.0
2	31.335297252740663	31.0	31.0	34.0	28.0	34.0
3	31.1900871767716	31.0	31.0	34.0	28.0	34.0
4	34.98800398267775	35.0	35.0	37.0	32.0	37.0
5	35.32610658975506	35.0	35.0	37.0	33.0	37.0
6	35.28964041081218	37.0	35.0	37.0	33.0	37.0
7	35.58369192856543	37.0	35.0	37.0	33.0	37.0
8	35.27688350324835	37.0	35.0	37.0	32.0	37.0
9	37.28311457738888	39.0	37.0	39.0	34.0	39.0
10	36.756045564301225	39.0	35.0	39.0	32.0	39.0
11	36.91406053190341	39.0	37.0	39.0	33.0	39.0
12	37.034790163951286	39.0	37.0	39.0	33.0	39.0
13	37.12239193730539	39.0	37.0	39.0	33.0	39.0
14	38.170980948610776	40.0	37.0	41.0	33.0	41.0
15	38.292877621772426	40.0	38.0	41.0	34.0	41.0
16	38.283104295088314	40.0	38.0	41.0	34.0	41.0
17	38.167082242981046	40.0	37.0	41.0	34.0	41.0
18	38.03761436917229	39.0	37.0	41.0	34.0	41.0
19	37.79253573527875	39.0	37.0	41.0	33.0	41.0
20	37.78590536513306	39.0	35.0	41.0	34.0	41.0
21	37.662613726528505	39.0	35.0	41.0	33.0	41.0
22	37.82119936181188	39.0	35.0	41.0	33.0	41.0
23	37.90491442555357	40.0	36.0	41.0	34.0	41.0
24	37.78581796557828	40.0	35.0	41.0	33.0	41.0
25	37.47655378414366	39.0	35.0	41.0	33.0	41.0
26	37.5739237430316	39.0	35.0	41.0	33.0	41.0
27	37.67612295575012	39.0	35.0	41.0	33.0	41.0
28	37.58803620055284	39.0	35.0	41.0	33.0	41.0
29	37.57857134288559	40.0	35.0	41.0	33.0	41.0
30	37.28989232717595	39.0	35.0	41.0	33.0	41.0
31	37.187833639231776	39.0	35.0	41.0	33.0	41.0
32	36.99878154738341	39.0	35.0	41.0	32.0	41.0
33	36.77024199394373	39.0	35.0	41.0	31.0	41.0
34	36.495541765848024	39.0	35.0	41.0	30.0	41.0
35	36.34164657333765	39.0	35.0	41.0	30.0	41.0
36	36.15490114424868	39.0	35.0	41.0	29.0	41.0
37	36.11156981596396	39.0	35.0	41.0	29.0	41.0
38	35.91901831449102	39.0	35.0	41.0	28.0	41.0
39	35.879049298490045	39.0	35.0	41.0	28.0	41.0
40	35.75668306693606	39.0	35.0	41.0	27.0	41.0
41	35.6064586557263	39.0	35.0	41.0	26.0	41.0
42	35.56879801620148	39.0	35.0	41.0	26.0	41.0
43	35.51546715062028	39.0	35.0	40.0	26.0	41.0
44	35.424991474259116	39.0	35.0	40.0	26.0	41.0
45	35.41118405832121	38.0	35.0	40.0	26.0	41.0
46	35.325186323854766	38.0	35.0	40.0	25.0	41.0
47	35.314619546310624	38.0	35.0	40.0	25.0	41.0
48	35.20356041794124	38.0	35.0	40.0	25.0	41.0
49	35.194544554065196	38.0	35.0	40.0	26.0	41.0
50	35.06678182843296	38.0	35.0	40.0	24.0	41.0
51	33.94978295777231	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	16.0
10	11.0
11	16.0
12	15.0
13	17.0
14	18.0
15	26.0
16	65.0
17	106.0
18	204.0
19	407.0
20	739.0
21	1159.0
22	1753.0
23	2457.0
24	3676.0
25	6134.0
26	8965.0
27	10284.0
28	9956.0
29	9254.0
30	9729.0
31	11598.0
32	15048.0
33	20821.0
34	36318.0
35	43041.0
36	46787.0
37	71553.0
38	126295.0
39	147028.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.788140051788524	21.178797210754603	22.917020120748482	18.116042616708395
2	37.117734055150834	24.374364853725705	22.995851091723264	15.5120499994002
3	30.410246655253314	23.087020823372352	30.979029248003947	15.523703273370385
4	28.110267391226113	23.131577459140708	31.802470151338326	16.95568499829485
5	24.908701739593884	27.475335331527074	28.921712277238242	18.694250651640797
6	24.557903918756114	33.797750575380405	27.798028197495572	13.846317308367908
7	73.86787586521275	5.784479552788474	15.496969291909371	4.850675290089405
8	73.8329160433022	6.704059966376877	14.429837865257305	5.033186125063622
9	67.45257717295001	7.798953604546148	16.726561067439896	8.021908155063947
10	37.80921876794047	23.30466285193316	23.378524044303006	15.507594335823363
11	29.039787362024377	22.77580985969801	29.33231881301122	18.852083965266388
12	25.459147563009083	20.833654655225896	33.180812541664736	20.526385240100286
13	23.35950178826344	21.21170057255277	35.68695193195859	19.7418457072252
14	19.861634508771658	25.48416782770977	33.176870993116	21.47732667040257
15	19.36568487833468	24.26777167123372	36.42642071403723	19.940122736394375
16	21.399695301160016	23.46335302393891	34.35128108896418	20.785670585936895
17	21.052153542166856	24.182085833217656	32.567644684821786	22.198115939793702
18	21.09174039933028	24.78942705307552	32.77774635963717	21.341086187957025
19	22.986254277865463	25.83445153351944	30.583674791397826	20.595619397217266
20	23.5737163833036	25.137311555420744	31.485775294030955	19.803196767244703
21	23.52693191574683	26.581460669343492	30.69489500914268	19.196712405767
22	21.464816538052222	23.61724478901576	31.290754326706388	23.627184346225626
23	21.404322336412882	26.102648206509727	30.9665191156536	21.52651034142379
24	21.457104812630778	24.327237642816872	31.92911381992607	22.28654372462628
25	21.93043338183152	26.255854484882445	29.82706884171598	21.986643291570058
26	20.350557900491324	26.199987318495978	31.47343653335664	21.976018247656064
27	21.855201216053413	25.664279459219543	30.90002690535314	21.58049241937391
28	20.04294574201365	26.441278638349207	32.490870173959394	21.02490544567775
29	21.989556610062603	25.803947375185725	29.895960255480897	22.31053575927078
30	21.431227689549925	25.61115423964958	31.6215016614484	21.336116409352098
31	22.873663086712355	26.929345171688713	28.38446207287752	21.812529668721414
32	23.50362536780646	27.97796845732931	28.109239161169917	20.40916701369431
33	22.68481149972495	26.242658865827973	28.17281805297784	22.899711581469237
34	21.711934494890553	28.641690958601746	27.855094965614274	21.79127958089343
35	23.65254735427838	26.280189262879013	28.016869827788604	22.050393555054008
36	23.696247131666574	29.106622315676912	26.126811612830252	21.07031893982626
37	24.009343183777272	28.35738534806444	27.624085946322964	20.00918552183532
38	23.523333110550155	27.75672762357183	26.79293331756714	21.927005948310878
39	25.15958987330492	25.752364500700054	26.533990715082595	22.554054910912434
40	23.873959559711892	25.1482793426868	29.40772235046536	21.57003874713595
41	22.699035348835615	27.17577764182291	28.124833983688845	22.00035302565263
42	23.29986444500426	26.902953933579766	28.291407252792073	21.505774368623904
43	23.964615176332888	24.658670464263007	29.179626649666595	22.19708770973751
44	23.057202151742764	25.829481754914514	28.058855888416474	23.05446020492625
45	22.401191375891774	24.720021524282508	28.7374877255037	24.14129937432201
46	23.915602876987695	25.83976405547644	27.5767873637381	22.667845703797767
47	22.71565840141073	25.137482927096777	30.052593967374257	22.09426470411823
48	22.186805409175584	25.316223585198284	29.289647265679225	23.207323739946908
49	22.23993062874554	24.229384415802524	30.328330994109955	23.202353961341977
50	21.460360874475388	23.68150916752781	31.122124597490775	23.736005360506027
51	21.77345692658609	22.97168768540273	28.95770032920499	26.297155058806194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	170.0
1	353.5
2	537.0
3	6558.5
4	12580.0
5	8600.5
6	4621.0
7	4472.0
8	4323.0
9	4299.5
10	4276.0
11	4102.0
12	3928.0
13	3833.5
14	3739.0
15	3544.5
16	3350.0
17	3166.5
18	2983.0
19	2830.0
20	2677.0
21	2719.5
22	2762.0
23	2758.5
24	2755.0
25	3089.5
26	4014.5
27	4605.0
28	5454.5
29	6304.0
30	7496.0
31	8688.0
32	9759.0
33	10830.0
34	11857.5
35	12885.0
36	13451.5
37	14018.0
38	15478.5
39	16939.0
40	19256.5
41	21574.0
42	23810.0
43	26046.0
44	29773.5
45	33501.0
46	45096.5
47	56692.0
48	56654.0
49	56616.0
50	55325.5
51	54035.0
52	47296.5
53	40558.0
54	35898.5
55	31239.0
56	28693.0
57	26147.0
58	25480.5
59	24814.0
60	24211.5
61	23609.0
62	21451.5
63	19294.0
64	16753.0
65	14212.0
66	12088.5
67	9965.0
68	8441.5
69	6918.0
70	5610.0
71	4302.0
72	3763.5
73	3225.0
74	2577.0
75	1528.0
76	1127.0
77	948.5
78	770.0
79	538.5
80	307.0
81	236.0
82	165.0
83	105.5
84	46.0
85	29.5
86	13.0
87	11.0
88	9.0
89	8.5
90	8.0
91	6.5
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	583527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.45133065162767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1230889006072	27.383365530876542
2	8.379747263795405	6.109058765793571
3	3.219332859984361	3.5204689967082037
4	1.8776964159172607	2.7377813167990506
5	1.3167781615619576	2.399915808096866
6	1.057214990436146	2.3122135911747206
7	0.9143131994918485	2.332955292767749
8	0.8074348727561562	2.354566042119165
9	0.728145731323092	2.3887692733526342
>10	6.481331111851901	40.178135363341134
>50	0.055249898488507636	1.3496789151744486
>100	0.03069438804879056	2.2426943738854455
>500	0.005666656255161335	1.437293577553417
>1k	0.0028333281275806674	1.9863913634690338
>5k	4.722213545967779E-4	1.2667117888880954
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7359	1.2611241639204356	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	2836	0.48601007322711715	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	2612	0.44762281779592034	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	2277	0.39021330632515716	No Hit
CGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTG	1506	0.2580857441043859	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1274	0.21832751526493205	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT	1035	0.17736968469325326	No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	966	0.16554503904703638	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	954	0.16348857893465082	No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	917	0.15714782692146206	Illumina Single End Adapter 2 (95% over 22bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	789	0.13521225238934959	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	698	0.11961742987042587	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC	676	0.11584725299771904	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	596	0.10213751891514873	No Hit
CGTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	589	0.10093791718292384	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17017207429990386	0.0
2	0.0	0.0	0.0	0.6764040052988122	0.0
3	0.0	0.0	0.0	1.11254492080058	0.0
4	0.0	0.0	0.0	1.5778190212278096	0.0
5	0.0	0.0	0.0	2.994548666985418	0.0
6	0.0	0.0	0.0	4.26098535286285	0.0
7	0.0	0.0	0.0	5.394437618139349	0.0
8	0.0	0.0	0.0	7.028809292457761	0.0
9	0.0	0.0	0.0	7.849508248975626	0.0
10	0.0	0.0	0.0	9.447549127975227	0.0
11	0.0	0.0	0.0	10.775850988900256	0.0
12	0.0	0.0	0.0	12.207147227120595	0.0
13	0.0	0.0	0.0	12.719205795104596	0.0
14	0.0	0.0	0.0	12.974720964068501	0.0
15	0.0	0.0	0.0	13.323805068145948	0.0
16	0.0	0.0	0.0	13.856256865577771	0.0
17	0.0	0.0	0.0	14.476279589462013	0.0
18	0.0	0.0	0.0	15.15782474504179	0.0
19	0.0	0.0	0.0	15.63852229631191	0.0
20	0.0	0.0	0.0	16.065066397955878	0.0
21	0.0	0.0	0.0	16.53719536542439	0.0
22	0.0	0.0	0.0	17.06176406575874	0.0
23	1.7137167603212876E-4	0.0	0.0	17.590445686317857	0.0
24	1.7137167603212876E-4	0.0	0.0	18.018532133046115	0.0
25	1.7137167603212876E-4	0.0	0.0	18.418684996581135	0.0
26	1.7137167603212876E-4	0.0	0.0	18.787305471726246	0.0
27	1.7137167603212876E-4	0.0	0.0	19.16775059251757	0.0
28	1.7137167603212876E-4	0.0	0.0	19.54528239481635	0.0
29	1.7137167603212876E-4	0.0	0.0	19.920757737002745	0.0
30	1.7137167603212876E-4	0.0	0.0	20.367009581390406	0.0
31	1.7137167603212876E-4	0.0	0.0	20.742313551900768	0.0
32	1.7137167603212876E-4	0.0	0.0	21.138010751858953	0.0
33	1.7137167603212876E-4	0.0	0.0	21.5249679963395	0.0
34	1.7137167603212876E-4	0.0	0.0	21.881592454162362	0.0
35	1.7137167603212876E-4	0.0	0.0	22.289114299766762	0.0
36	1.7137167603212876E-4	0.0	0.0	22.66904530552999	0.0
37	1.7137167603212876E-4	0.0	0.0	23.050690028053545	0.0
38	1.7137167603212876E-4	0.0	0.0	23.427536343648196	0.0
39	1.7137167603212876E-4	0.0	0.0	23.800269739018074	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	45	3.8380676E-10	45.000004	1
GCTACGA	50	2.1827873E-11	45.000004	10
GACCGAA	20	7.030428E-4	45.0	11
GACCCGT	20	7.030428E-4	45.0	12
ACGATAG	20	7.030428E-4	45.0	1
CGTTTTT	4765	0.0	43.394543	1
ATAGCGG	80	0.0	42.1875	2
CTACGAA	55	6.002665E-11	40.909092	11
CGTAAGG	50	1.0804797E-9	40.500004	2
ACGGGAC	155	0.0	39.193546	5
TCGTGTA	180	0.0	38.750004	17
GTTTTTT	5470	0.0	38.007317	2
CCTCGTG	185	0.0	37.7027	15
GTAGGGA	335	0.0	37.611942	4
TCTACGG	30	1.1392332E-4	37.499996	2
CGAATAT	60	1.546141E-10	37.499996	14
GCGATTC	30	1.1392332E-4	37.499996	9
GCTAACG	30	1.1392332E-4	37.499996	1
CGATCCC	30	1.1392332E-4	37.499996	43
TGCGTTG	30	1.1392332E-4	37.499996	1
>>END_MODULE