SRR3550946_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288630
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	9865	3.417870630218619	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	7562	2.6199632747808614	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	6877	2.3826352077053667	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2089	0.7237639884973842	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACGAGAT	2060	0.7137165228839691	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	1503	0.5207358902400998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	1473	0.5103419602951876	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTA	1437	0.497869244361293	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1349	0.4673803831895506	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTC	1274	0.44139555832727023	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCT	982	0.34022797353012507	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTC	954	0.33052697224820704	No Hit
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	611	0.2116897065447112	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	553	0.19159477531788102	Illumina Single End Adapter 2 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	517	0.17912205938398643	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATG	431	0.1493261268752382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	426	0.14759380521775284	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGACGAGATCGT	396	0.13719987527284067	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTCTTA	366	0.1268059453279285	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGACGAGA	361	0.12507362367044314	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTAT	345	0.11953019436648997	No Hit
CGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	299	0.10359283511762465	Illumina Single End Adapter 2 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	50	2.1827873E-11	45.0	33
AGCAATC	20	7.024498E-4	45.0	11
CGAACAC	25	3.8835227E-5	45.0	34
CCGATCG	75	0.0	45.0	40
CGTGCGT	20	7.024498E-4	45.0	11
GCGCGAC	45	3.8198777E-10	45.0	9
ATTAAGG	25	3.8835227E-5	45.0	2
ATAGACG	25	3.8835227E-5	45.0	1
CACGAGC	20	7.024498E-4	45.0	41
CCGTCAG	25	3.8835227E-5	45.0	30
TTGAGTA	20	7.024498E-4	45.0	37
ATCCGGC	35	1.2076998E-7	45.0	6
GTAGACT	20	7.024498E-4	45.0	32
GCGATAC	45	3.8198777E-10	45.0	33
GCAATCA	20	7.024498E-4	45.0	12
ATACGGT	20	7.024498E-4	45.0	28
AGGGCGC	35	1.2076998E-7	45.0	6
CGATCGA	50	2.1827873E-11	45.0	41
TATCGCG	25	3.8835227E-5	45.0	1
CTCGATC	30	2.1598335E-6	44.999996	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content