##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	288630
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265512940442783	31.0	31.0	33.0	28.0	34.0
2	31.186623012160897	31.0	31.0	34.0	28.0	34.0
3	31.15249974015175	31.0	31.0	34.0	28.0	34.0
4	35.032955687212	35.0	35.0	37.0	32.0	37.0
5	35.22286664587881	35.0	35.0	37.0	33.0	37.0
6	35.12086408204275	36.0	35.0	37.0	33.0	37.0
7	35.45408654679001	37.0	35.0	37.0	33.0	37.0
8	34.89436302532654	37.0	35.0	37.0	32.0	37.0
9	36.731375809860374	39.0	37.0	39.0	32.0	39.0
10	36.39091916987146	37.0	35.0	39.0	32.0	39.0
11	36.603572047257735	38.0	35.0	39.0	32.0	39.0
12	36.66011502615806	39.0	35.0	39.0	32.0	39.0
13	36.49852406194782	39.0	35.0	39.0	32.0	39.0
14	37.5260125420088	39.0	36.0	41.0	32.0	41.0
15	37.48855974777397	39.0	36.0	41.0	32.0	41.0
16	37.81283650348197	39.0	36.0	41.0	33.0	41.0
17	37.4765790111908	39.0	36.0	41.0	32.0	41.0
18	37.497429234660295	39.0	36.0	41.0	32.0	41.0
19	37.372958458926654	39.0	36.0	40.0	32.0	41.0
20	37.090222776565156	39.0	35.0	41.0	31.0	41.0
21	37.34689394726813	39.0	35.0	41.0	32.0	41.0
22	37.44238644631535	39.0	35.0	41.0	32.0	41.0
23	37.438762429407895	39.0	35.0	41.0	33.0	41.0
24	37.33996466063819	39.0	35.0	41.0	32.0	41.0
25	37.27036344108374	39.0	35.0	41.0	32.0	41.0
26	37.11356407857811	39.0	35.0	41.0	32.0	41.0
27	37.113965977202646	39.0	35.0	41.0	32.0	41.0
28	36.977330838790145	39.0	35.0	41.0	31.0	41.0
29	36.96440425458199	39.0	35.0	41.0	31.0	41.0
30	36.80080726189239	39.0	35.0	41.0	31.0	41.0
31	36.796015660187784	39.0	35.0	41.0	31.0	41.0
32	36.58587464920486	39.0	35.0	41.0	31.0	41.0
33	36.492835117624644	39.0	35.0	41.0	30.0	41.0
34	36.25616186813568	39.0	35.0	41.0	30.0	41.0
35	36.33965284273984	39.0	35.0	41.0	30.0	41.0
36	36.312188615182066	39.0	35.0	41.0	30.0	41.0
37	36.216138308561135	39.0	35.0	41.0	30.0	41.0
38	36.016176419637596	39.0	35.0	41.0	29.0	41.0
39	35.99333749090531	39.0	35.0	41.0	29.0	41.0
40	35.72317499913384	39.0	35.0	40.0	28.0	41.0
41	35.47763572740186	38.0	35.0	40.0	26.0	41.0
42	35.69754010324637	39.0	35.0	40.0	28.0	41.0
43	35.635301250736234	38.0	35.0	40.0	28.0	41.0
44	35.75087828708035	39.0	35.0	40.0	28.0	41.0
45	35.756511797110484	38.0	35.0	40.0	28.0	41.0
46	35.6486228042823	38.0	35.0	40.0	28.0	41.0
47	35.32487613900149	38.0	35.0	40.0	27.0	41.0
48	35.40131656445969	38.0	35.0	40.0	27.0	41.0
49	35.455150192287704	38.0	35.0	40.0	28.0	41.0
50	35.274320063749435	38.0	35.0	40.0	27.0	41.0
51	34.02450542216679	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	11.0
10	9.0
11	5.0
12	10.0
13	2.0
14	15.0
15	15.0
16	30.0
17	61.0
18	144.0
19	382.0
20	846.0
21	1401.0
22	1733.0
23	2089.0
24	2250.0
25	2727.0
26	3169.0
27	3452.0
28	3585.0
29	3956.0
30	4987.0
31	6787.0
32	8997.0
33	12486.0
34	21299.0
35	24593.0
36	24021.0
37	34463.0
38	57781.0
39	67308.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.049197935072584	18.038318955063577	20.20961092055573	11.702872189308112
2	27.694279873886984	36.578318262134914	20.907390084190833	14.82001177978727
3	27.322870110522125	35.55590201988706	23.409901950594186	13.71132591899664
4	24.8993521117001	21.164466618161658	38.24966219727679	15.686519072861449
5	34.14302047604199	24.40598690364827	22.045525413158714	19.405467207151023
6	21.65887121920798	41.909364930880365	23.091847694279874	13.339916155631778
7	67.81381006825347	5.568374735820948	22.074281952672973	4.543533243252607
8	65.60371409763364	16.8052523992655	11.819977133354122	5.771056369746734
9	60.48435713543291	7.303814572289783	11.837646814260472	20.37418147801684
10	43.5152271073693	21.226830197831134	19.55513979835776	15.702802896441812
11	35.51155458545543	20.809687142708658	24.152375012992415	19.526383258843502
12	28.07677649585975	18.13117139590479	28.28500155908949	25.507050549145966
13	26.01358140179468	21.626996500710252	34.4738939126217	17.885528184873365
14	19.987527284066108	32.38298167203686	25.432907182205593	22.196583861691437
15	15.982053147628452	23.119218376468144	38.929425215674044	21.96930326022936
16	18.244465232304336	22.952222568686555	29.278661261823096	29.524650937186014
17	18.37196410629526	26.28139833004192	31.31587153102588	24.03076603263694
18	18.82132834424696	25.517790943422376	28.66888403838825	26.99199667394242
19	20.93025673006964	26.604996015660188	25.547240411599624	26.917506842670548
20	26.21660950005197	24.929494508540344	31.367148252087446	17.486747739320236
21	22.213214149603296	31.706336832623084	26.638949520146898	19.44149949762672
22	19.58701451685549	22.55517444479091	30.165956414787097	27.691854623566503
23	22.17960710944808	29.98094446176766	27.499913383917125	20.33953504486713
24	23.33125454734435	24.208502234694937	26.17364792294633	26.286595295014376
25	20.15660187783668	33.838131864324566	23.40713023594221	22.598136021896543
26	18.934622180646503	25.982053147628452	28.865329314347086	26.21799535737796
27	25.700377646121332	27.295152964002355	24.994629802861795	22.00983958701452
28	17.601773897377264	28.699719363891486	31.0771576066244	22.62134913210685
29	22.527110833939645	26.345840695700378	26.712053494092785	24.41499497626719
30	24.243148667844647	29.148737137511695	26.992689602605413	19.61542459203825
31	24.599660464955132	28.739562762013648	20.928177944080655	25.732598828950557
32	28.837958632158816	29.06974326993036	20.252918961992865	21.83937913591796
33	27.041541073346497	25.979974361639467	20.669715552783842	26.308769012230194
34	24.15861137095936	31.684856044070266	22.13491321068496	22.021619374285418
35	29.234660291722967	23.933063091154764	21.424661331115963	25.40761528600631
36	22.7453833627828	37.175276305304365	20.456639988913143	19.62270034299969
37	25.41177285798427	26.77511000242525	26.15286006305651	21.66025707653397
38	24.579219069396803	29.94906974326993	20.478467241797457	24.993243945535806
39	23.15525066694384	28.52752659113744	23.10882444652323	25.20839829539549
40	28.44853272355611	23.379413089422442	26.49828500155909	21.67376918546236
41	19.78103454249385	33.05546893947268	23.47261199459516	23.690884523438314
42	24.99844091050826	24.895541004053634	28.59889824342584	21.507119842012266
43	24.45241312406888	28.681703218653638	23.103627481550774	23.76225617572671
44	23.971174167619445	24.303779925856634	26.876624051553893	24.84842185497003
45	21.85912760281329	23.568582614419846	25.68790493018744	28.884384852579426
46	27.458684128468974	27.21061566711707	23.200637494369953	22.130062710044
47	21.403180542563145	24.507500952776912	33.193361743408516	20.89595676125143
48	22.748501541766274	27.298964071648825	24.67553615355299	25.27699823303191
49	21.46277240758064	22.316114056057927	31.89412050029449	24.326993036066938
50	23.052350760489208	22.99657000311818	28.3553338183834	25.595745418009212
51	20.30869971936389	23.30319093649309	25.615840349236045	30.772268994906977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	519.0
1	483.0
2	447.0
3	1443.0
4	2439.0
5	1657.0
6	875.0
7	856.5
8	838.0
9	808.5
10	779.0
11	792.5
12	806.0
13	803.0
14	800.0
15	752.0
16	704.0
17	679.5
18	655.0
19	610.5
20	566.0
21	548.0
22	530.0
23	704.0
24	878.0
25	1041.5
26	1351.5
27	1498.0
28	1883.5
29	2269.0
30	2929.5
31	3590.0
32	3683.0
33	3776.0
34	4382.5
35	4989.0
36	5551.5
37	6114.0
38	6793.5
39	7473.0
40	8944.5
41	10416.0
42	11649.5
43	12883.0
44	15583.5
45	18284.0
46	35444.0
47	52604.0
48	39962.5
49	27321.0
50	26468.0
51	25615.0
52	22084.0
53	18553.0
54	16815.0
55	15077.0
56	13820.5
57	12564.0
58	12278.0
59	11992.0
60	11821.5
61	11651.0
62	10611.5
63	9572.0
64	8359.0
65	7146.0
66	5629.5
67	4113.0
68	3640.5
69	3168.0
70	2679.0
71	2190.0
72	1913.0
73	1636.0
74	1250.0
75	741.5
76	619.0
77	446.0
78	273.0
79	229.5
80	186.0
81	125.0
82	64.0
83	51.0
84	38.0
85	35.0
86	32.0
87	21.5
88	11.0
89	6.5
90	2.0
91	4.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	288630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.516474378962684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67222918453048	23.849218722932473
2	9.063826044895896	5.713196826386723
3	3.0439944594683728	2.8780792017461803
4	1.553327616911813	1.9582164016214532
5	1.0608359167161356	1.671690399473374
6	0.8299804322494119	1.5694834216817377
7	0.7244464964931953	1.598239961195995
8	0.6452960446760329	1.6269965007102518
9	0.6035221951058638	1.7118802619270346
>10	6.683815931227052	40.05023732806707
>50	0.06156146252445969	1.2988947787825242
>100	0.040674537739375155	2.5250320479506634
>500	0.0054965591539696155	1.2531614870249108
>1k	0.007695182815557461	3.875203547794755
>5k	0.0032979354923817687	8.420469112704847
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGC	9865	3.417870630218619	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTC	7562	2.6199632747808614	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCG	6877	2.3826352077053667	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	2089	0.7237639884973842	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACGAGAT	2060	0.7137165228839691	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	1503	0.5207358902400998	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCT	1473	0.5103419602951876	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACGAGATCGTA	1437	0.497869244361293	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1349	0.4673803831895506	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTC	1274	0.44139555832727023	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCT	982	0.34022797353012507	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTC	954	0.33052697224820704	No Hit
CCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	611	0.2116897065447112	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	553	0.19159477531788102	Illumina Single End Adapter 2 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	517	0.17912205938398643	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATG	431	0.1493261268752382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	426	0.14759380521775284	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGACGAGATCGT	396	0.13719987527284067	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTCTTA	366	0.1268059453279285	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGACGAGA	361	0.12507362367044314	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTAT	345	0.11953019436648997	No Hit
CGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTG	299	0.10359283511762465	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23698160274399752	0.0
2	0.0	0.0	0.0	1.5441915254824516	0.0
3	0.0	0.0	0.0	1.8625922461282611	0.0
4	0.0	0.0	0.0	2.790423725877421	0.0
5	0.0	0.0	0.0	6.781692824723694	0.0
6	0.0	0.0	0.0	7.641617295499429	0.0
7	0.0	0.0	0.0	8.890274746214876	0.0
8	0.0	0.0	0.0	10.345771402834078	0.0
9	0.0	0.0	0.0	10.762567993625057	0.0
10	0.0	0.0	0.0	14.356788968575685	0.0
11	0.0	0.0	0.0	15.488341475245123	0.0
12	0.0	0.0	0.0	19.922391989744657	0.0
13	0.0	0.0	0.0	20.47812077746596	0.0
14	0.0	0.0	0.0	20.7930568547968	0.0
15	3.4646433149707235E-4	0.0	0.0	21.565325849703772	0.0
16	3.4646433149707235E-4	0.0	0.0	22.18930811073	0.0
17	3.4646433149707235E-4	0.0	0.0	22.801510584485328	0.0
18	3.4646433149707235E-4	0.0	0.0	23.358971693864117	0.0
19	3.4646433149707235E-4	0.0	0.0	24.562935245816444	0.0
20	3.4646433149707235E-4	0.0	0.0	25.059072168520252	0.0
21	3.4646433149707235E-4	0.0	0.0	25.510515192460936	0.0
22	3.4646433149707235E-4	0.0	0.0	26.097425770016976	0.0
23	3.4646433149707235E-4	0.0	0.0	26.553026365935626	0.0
24	3.4646433149707235E-4	0.0	0.0	26.95942902678169	0.0
25	3.4646433149707235E-4	0.0	0.0	27.278522676090496	0.0
26	3.4646433149707235E-4	0.0	0.0	27.580293108824446	0.0
27	3.4646433149707235E-4	0.0	0.0	27.971797803416138	0.0
28	3.4646433149707235E-4	0.0	0.0	28.276339950802065	0.0
29	6.929286629941447E-4	0.0	0.0	28.594394207116377	0.0
30	6.929286629941447E-4	0.0	0.0	28.93843328829297	0.0
31	6.929286629941447E-4	0.0	0.0	29.282472369469563	0.0
32	6.929286629941447E-4	0.0	0.0	29.625472057651663	0.0
33	6.929286629941447E-4	0.0	0.0	29.946298028617953	0.0
34	6.929286629941447E-4	0.0	0.0	30.2837542874961	0.0
35	6.929286629941447E-4	0.0	0.0	30.626753975678206	0.0
36	6.929286629941447E-4	0.0	0.0	30.967674877871325	0.0
37	0.0010393929944912171	0.0	0.0	31.347399785192113	0.0
38	0.0010393929944912171	0.0	0.0	31.631500537019715	0.0
39	0.0010393929944912171	0.0	0.0	31.947129543013546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	50	2.1827873E-11	45.0	33
AGCAATC	20	7.024498E-4	45.0	11
CGAACAC	25	3.8835227E-5	45.0	34
CCGATCG	75	0.0	45.0	40
CGTGCGT	20	7.024498E-4	45.0	11
GCGCGAC	45	3.8198777E-10	45.0	9
ATTAAGG	25	3.8835227E-5	45.0	2
ATAGACG	25	3.8835227E-5	45.0	1
CACGAGC	20	7.024498E-4	45.0	41
CCGTCAG	25	3.8835227E-5	45.0	30
TTGAGTA	20	7.024498E-4	45.0	37
ATCCGGC	35	1.2076998E-7	45.0	6
GTAGACT	20	7.024498E-4	45.0	32
GCGATAC	45	3.8198777E-10	45.0	33
GCAATCA	20	7.024498E-4	45.0	12
ATACGGT	20	7.024498E-4	45.0	28
AGGGCGC	35	1.2076998E-7	45.0	6
CGATCGA	50	2.1827873E-11	45.0	41
TATCGCG	25	3.8835227E-5	45.0	1
CTCGATC	30	2.1598335E-6	44.999996	16
>>END_MODULE