SRR3550945_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444061
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	7396	1.6655369419967079	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	6496	1.462862084263198	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	6137	1.3820173354561647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3049	0.6866173791438563	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	2871	0.6465327961698956	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	2047	0.46097270420054903	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	1568	0.3531046410290478	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT	1424	0.3206766637916863	No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	1393	0.31369564091419877	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	1367	0.307840589468564	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	1126	0.25356876645325754	No Hit
CGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	1120	0.25221760073503413	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA	1103	0.24838929786673453	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1003	0.2258698692296779	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT	987	0.22226676064774883	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	959	0.21596132062937298	No Hit
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	549	0.12363166321744085	TruSeq Adapter, Index 16 (95% over 21bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	546	0.12295608035832915	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	532	0.11980336034914123	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	509	0.1146238917626182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	472	0.10629170316690725	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	25	3.8869264E-5	45.0	6
CGGCGAA	20	7.0286053E-4	45.0	31
CCGATTT	20	7.0286053E-4	45.0	43
ACCGAGT	20	7.0286053E-4	45.0	40
ATTCGGA	20	7.0286053E-4	45.0	16
GCGTTTA	150	0.0	45.0	32
TCGATTA	20	7.0286053E-4	45.0	41
GCCGATA	20	7.0286053E-4	45.0	9
ACGATAG	20	7.0286053E-4	45.0	1
TAACCGA	20	7.0286053E-4	45.0	38
CGTAGCC	20	7.0286053E-4	45.0	29
TACGAGC	25	3.8869264E-5	45.0	39
AACGAGC	20	7.0286053E-4	45.0	15
TATAGCG	25	3.8869264E-5	45.0	1
ATAACCG	20	7.0286053E-4	45.0	37
CGCACGG	40	6.7993824E-9	45.0	2
CGCGTTT	20	7.0286053E-4	45.0	20
CGTTTAC	150	0.0	45.0	33
AGTCGTT	20	7.0286053E-4	45.0	44
GATAGAC	20	7.0286053E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content