SRR3550944_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718598
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12667	1.7627379981575233	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	8107	1.1281690180045032	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGC	4801	0.6681065073935636	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCG	4568	0.6356822590655693	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	3694	0.5140565378695737	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	2904	0.4041202452553444	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2480	0.345116462890239	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	2271	0.31603205129989226	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2129	0.29627135060214477	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCT	2113	0.2940447927770464	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	2046	0.2847210818844472	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1911	0.26593450023518017	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	1455	0.20247760221987818	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1204	0.1675484763386483	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	1159	0.1612862824555593	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCT	1088	0.15140593210668551	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTT	1075	0.14959685387379312	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTCTCAT	1047	0.14570037767987107	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTC	988	0.13748994569982104	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	841	0.11703344568173026	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.11174537084712174	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGT	801	0.11146705111898447	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	794	0.11049293207050395	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGTA	30	2.164361E-6	45.000004	41
AACGTCG	20	7.03152E-4	45.0	1
GCGATAT	50	2.1827873E-11	45.0	9
CATCGTT	25	3.8893406E-5	45.0	36
GCGTGAT	20	7.03152E-4	45.0	15
AATAGGC	20	7.03152E-4	45.0	3
GTACGAG	50	2.1827873E-11	45.0	1
CGTTTTT	8935	0.0	43.614998	1
CGTTTCT	335	0.0	40.298508	1
AAGTACG	40	3.455807E-7	39.375	1
TACCCGC	40	3.455807E-7	39.375	28
TTACCCG	40	3.455807E-7	39.375	27
CGATATG	40	3.455807E-7	39.375	10
GCCCGAA	40	3.455807E-7	39.375	25
AACGTAG	35	6.2450217E-6	38.571426	1
TCGTTGA	35	6.2450217E-6	38.571426	24
GAAGCGT	35	6.2450217E-6	38.571426	29
CGACGGT	35	6.2450217E-6	38.571426	28
TACGGTT	35	6.2450217E-6	38.571426	33
GCGTTTA	35	6.2450217E-6	38.571426	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content